Biological and virulence study of highly pathogenic<em> Beauveria bassiana</em> on pine sawyer <em>Monochamus alternatus</em>

ZHAN Weijun; LUO Youbo; ZHANG Libin

doi:10.11833/j.issn.2095-0756.2013.01.012

Volume 30 Issue 1

Apr. 2013

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ZHAN Weijun, LUO Youbo, ZHANG Libin. Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus[J]. Journal of Zhejiang A&F University, 2013, 30(1): 83-89. doi: 10.11833/j.issn.2095-0756.2013.01.012

Citation:

ZHAN Weijun, LUO Youbo, ZHANG Libin. Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus[J]. Journal of Zhejiang A&F University, 2013, 30(1): 83-89. doi: 10.11833/j.issn.2095-0756.2013.01.012

Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus

doi: 10.11833/j.issn.2095-0756.2013.01.012

1.
1． Zhejiang Development Landscape Co. Ltd.，Hangzhou 310020，Zhejiang，China;
2.
2． Seedling Management Center of Hangzhou City，Hangzhou 310016，Zhejiang，China;
3.
Forest Enterprise of Pingyang County，Pingyang 325400，Zhejiang，China

More Information

Corresponding author: ZHAN Weijun
Received Date: 2011-12-20
Rev Recd Date: 2012-07-20
Publish Date: 2013-02-20

Abstract

The colony growth and sporogenous conditions of the highly pathogenic strain Bxs of Beauveria bassiana on pine sawyer Monochamus alternatus，and the virulence of Bxs to pine sawyer Monochamus alternatus were studied. The results showed that the growth temperature for Bxs ranged from 20 to 30 ℃，and 25 ℃ was optimal for both colony growth and sporulation，but the colony growth stopped at 35 ℃. For growth and sporulation，DPSA was the desirable culture medium. Virulence tests indicated that great variations existed in disease susceptibility among different developmental stages of Monochamus alternatus，and higher disease sus-ceptibility occurred in the juvenile stages. The equation of dose-mortality and LC50 of the third instar were y=-1.388 4+0.996 7x and 3 936 sporesmL-1 respectively. Based on the Three-dimensional Relation of time-dosage-mortality，a complementary log-log model （CLL model） of Bxs virulence to third instar M. alternatus was constructed.［Ch，3 fig. 7 tab. 11 ref.］
- forest protection,
- Monochamus alternatus,
- Beauveria bassiana,
- biology,
- virulence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus

1. 1． Zhejiang Development Landscape Co. Ltd.，Hangzhou 310020，Zhejiang，China;

2. 2． Seedling Management Center of Hangzhou City，Hangzhou 310016，Zhejiang，China;

3. Forest Enterprise of Pingyang County，Pingyang 325400，Zhejiang，China

Corresponding author: ZHAN Weijun

Received Date: 2011-12-20

Rev Recd Date: 2012-07-20

Publish Date: 2013-02-20

Key words:

Abstract: The colony growth and sporogenous conditions of the highly pathogenic strain Bxs of Beauveria bassiana on pine sawyer Monochamus alternatus，and the virulence of Bxs to pine sawyer Monochamus alternatus were studied. The results showed that the growth temperature for Bxs ranged from 20 to 30 ℃，and 25 ℃ was optimal for both colony growth and sporulation，but the colony growth stopped at 35 ℃. For growth and sporulation，DPSA was the desirable culture medium. Virulence tests indicated that great variations existed in disease susceptibility among different developmental stages of Monochamus alternatus，and higher disease sus-ceptibility occurred in the juvenile stages. The equation of dose-mortality and LC50 of the third instar were y=-1.388 4+0.996 7x and 3 936 sporesmL-1 respectively. Based on the Three-dimensional Relation of time-dosage-mortality，a complementary log-log model （CLL model） of Bxs virulence to third instar M. alternatus was constructed.［Ch，3 fig. 7 tab. 11 ref.］

Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus

doi: 10.11833/j.issn.2095-0756.2013.01.012

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus

doi: 10.11833/j.issn.2095-0756.2013.01.012

1. 1． Zhejiang Development Landscape Co. Ltd.，Hangzhou 310020，Zhejiang，China;

2. 2． Seedling Management Center of Hangzhou City，Hangzhou 310016，Zhejiang，China;

3. Forest Enterprise of Pingyang County，Pingyang 325400，Zhejiang，China

Corresponding author: ZHAN Weijun

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Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus

doi: 10.11833/j.issn.2095-0756.2013.01.012

Abstract

Relative Article

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Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Biological and virulence study of highly pathogenic Beauveria bassiana on pine sawyer Monochamus alternatus

doi: 10.11833/j.issn.2095-0756.2013.01.012

1. 1． Zhejiang Development Landscape Co. Ltd.，Hangzhou 310020，Zhejiang，China; 2. 2． Seedling Management Center of Hangzhou City，Hangzhou 310016，Zhejiang，China; 3. Forest Enterprise of Pingyang County，Pingyang 325400，Zhejiang，China

Corresponding author: ZHAN Weijun

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1. 1． Zhejiang Development Landscape Co. Ltd.，Hangzhou 310020，Zhejiang，China;

2. 2． Seedling Management Center of Hangzhou City，Hangzhou 310016，Zhejiang，China;

3. Forest Enterprise of Pingyang County，Pingyang 325400，Zhejiang，China