Analysis of ESTs associated with oil metabolism in seeds of <em>Carya cathayensis</em>

HUANG Yinzhi; ZENG Yanru; ZHOU Qin; XIA Guohua; HUANG Youjun

doi:10.11833/j.issn.2095-0756.2015.02.009

Volume 32 Issue 2

Mar. 2015

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2015 > 32(2): 229-236

HUANG Yinzhi, ZENG Yanru, ZHOU Qin, XIA Guohua, HUANG Youjun. Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009

Citation:

HUANG Yinzhi, ZENG Yanru, ZHOU Qin, XIA Guohua, HUANG Youjun. Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.02.009

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
2.
Agricultural Office of Toutuo People’s Government, Huangyan District, Huangyan 318026, Zhejiang, China;
3.
Jinhua Institute of Agricultural Sciences, Jinhua 321017, Zhejiang, China

Received Date: 2014-06-12
Rev Recd Date: 2014-08-24
Publish Date: 2015-04-20

Abstract

Carya cathayensis is an important oil species with dry nuts that are high oil content, for which there must be some special oil-synthesizing mechanism. In order to explore the mechanism, random cloning and sequencing of cDNAs from a fatty acid metabolism-associated cDNA library, sequence assembly and annotation, Gene Ontology （GO）- and Arabidopsis thaliana-associated MIPS Functional Catalogue （FunCat）-based classification of assembled sequences, and protein analysis of fatty acid metabolism-associated cDNA sequences were performed. A total of 1 010 expressed sequence tags （ESTs） was obtained, based on which 188 unigenes were assembled. There were 92 contigs and 96 singlets among the unigenes, which were classified into categories of cellular component, molecular function, and biological process based on classification of GO and found to overlap one another in functions. But against MIPS Functional Catalogue in Arabidopsis thaliana, these unigenes were classified into nine categories and 143 full-length cDNAs were obtained, from which 14 cDNA sequences were associated with metabolism of fatty acids. It had been found through proteomic analysis that 12 full-length fatty acid metabolism-related cDNAs had a phosphorylation site; they were all non-secretory proteins with no transmembrane domain and signal peptide except oleosins, belonging to certain protein family; and oleosins had different transmembrane domains and a signal peptide, belonging to a super oleosin family. ［Ch, 4 tab. 35 ref.］
- cash forestry,
- Carya cathayensis,
- expressed sequence tag,
- functional classification,
- fatty acid metabolism,
- protein analysis

Relative Article

[1]	HE Guoqing, YU Chunlian, RAO Ying, ZHANG Fuyang, SHEN Xiaofei, HUANG Jianqin, LIU Li, XIA Guohua. Dynamic changes in composition of mineral elements and fatty acids for hickory nuts (Carya cathayensis) during maturity . Journal of Zhejiang A&F University, 2019, 36(6): 1208-1216. doi: 10.11833/j.issn.2095-0756.2019.06.019
[2]	CHEN Junhao, LU Yang, NI Yanjie, ZHANG Zhijun, ZHONG Yaping, BAO Jingjing, LUO Shuping. Development of novel EST-SSR markers in pineapple (Ananas comosus) by data mining from public EST sequences . Journal of Zhejiang A&F University, 2017, 34(2): 332-339. doi: 10.11833/j.issn.2095-0756.2017.02.018
[3]	MA Shanshan, ZHAO Keli, DING Lizhong, HUANG Sha, CAI Ling, ZHAO Weiming, YE Zhengqian. Soil fertility in Carya cathayensis orchards for major towns of Lin'an City, China . Journal of Zhejiang A&F University, 2016, 33(6): 953-960. doi: 10.11833/j.issn.2095-0756.2016.06.005
[4]	HOU Chuanming, ZHENG Yawen, WANG Zhengjia, XU Yingwu. Cloning and analysis of a MADS-like gene in Carya cathayensis . Journal of Zhejiang A&F University, 2015, 32(1): 33-39. doi: 10.11833/j.issn.2095-0756.2015.01.005
[5]	LI Shunfu, HU Hengkang, ZHANG Qiulu, XU Yanling, ZHANG Qixiang. ISSR analysis of DNA methylation variety on immature embryos of Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(4): 521-527. doi: 10.11833/j.issn.2095-0756.2014.04.005
[6]	WANG Xuan, MA Liangjin, LÜ Quan, MENG Xianjing, ZHANG Xingyao. Identification of the pathogens causing stem canker on Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(2): 238-245. doi: 10.11833/j.issn.2095-0756.2014.02.012
[7]	SHEN Chen, QIU Jiani, HUANG Jianqin. Cloning and expression analysis of COP1 E3 ligase in Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(6): 831-837. doi: 10.11833/j.issn.2095-0756.2014.06.002
[8]	ZHU Hangrui, ZHUANG Xiaowei, PAN Xin, CHEN Shunwei, ZHANG Jiangli. Combustion performance of charcoal briquettes made from three kinds of epicarps . Journal of Zhejiang A&F University, 2013, 30(1): 90-94. doi: 10.11833/j.issn.2095-0756.2013.01.013
[9]	YANG Xihong, HUANG Youjun, CHEN Fangfang, HUANG Jianqin. Identification and expression of FLOWERING LOCUS C homologous genes in Carya cathayensis . Journal of Zhejiang A&F University, 2013, 30(1): 1-8. doi: 10.11833/j.issn.2095-0756.2013.01.001
[10]	SONG Shuang, HUANG Yin-zhi, DONG Lei-ming, HUANG You-jun, ZENG Yan-ru, WU Zhi-min. Simple sequence repeat （SSR） markers in Carya cathayensis . Journal of Zhejiang A&F University, 2012, 29(4): 626-633. doi: 10.11833/j.issn.2095-0756.2012.04.023
[11]	YANG Jian-hua, LI Shu-fang, FAN Zhi-yuan, XI Xue-liang, ZOU Wei-lie, LIU Jiao, PAN Li. Principal component analysis for major economic characteristics of Carya illinoensis and selection of superior cultivars . Journal of Zhejiang A&F University, 2011, 28(6): 907-910. doi: 10.11833/j.issn.2095-0756.2011.06.011
[12]	JIN Zhi-feng, ZHAO Hong-bo, LI Bo, LI Ren-zhong, HUANG Jing-feng. Optimum areas for Carya cathayensis planting in Zhejiang Province based on GIS . Journal of Zhejiang A&F University, 2011, 28(2): 256-261. doi: 10.11833/j.issn.2095-0756.2011.02.014
[13]	HU Heng-kang, JIANG Xiang-mei, ZHANG Qi-xiang, CHEN Bei, HUANG Jian-qin. Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources . Journal of Zhejiang A&F University, 2011, 28(6): 911-917. doi: 10.11833/j.issn.2095-0756.2011.06.012
[14]	LI Yuan-chun, SHEN Lin, ZENG Yan-ru. Establishment of a SRAP analysis protocol in Carya cathayensis and a comparison among SRAP，RAPD，ISSR analysis protocols . Journal of Zhejiang A&F University, 2011, 28(3): 505-512. doi: 10.11833/j.issn.2095-0756.2011.03.025
[15]	HUANG Yin-zhi, ZHOU Qin, HUANG You-jun, ZENG Yan-ru. Construction of a fat metabolism-related cDNA library in Carya cathayensis . Journal of Zhejiang A&F University, 2011, 28(1): 80-85. doi: 10.11833/j.issn.2095-0756.2011.01.013
[16]	XU Yan, SHEN Yue-qin, HUANG Jian-qin, LIN Jian-hua. Farmers’ willingness to adopt ecological management model for Carya cathayensis . Journal of Zhejiang A&F University, 2010, 27(5): 750-756. doi: 10.11833/j.issn.2095-0756.2010.05.018
[17]	ZHENG Bing-song, CHEN Miao, CHU Huai-liang, AI Xue, HUANG You-jun, LI Xue-qin, HUANG Jian-qin. Auxin response factor fragments cloned by cDNA-AFLP during Carya cathayensis grafting process . Journal of Zhejiang A&F University, 2009, 26(4): 467-472.
[18]	HUANG You-jun, ZHOU Li, CHEN Fang-fang, ZHOU Qin, HUANG Jian-qin, HUANG Min-ren, WANG ming-xiu. Gene expression with cDNA-AFLP （amplified fragment length polymorphism） during flowering of Carya cathayensis . Journal of Zhejiang A&F University, 2009, 26(3): 297-301.
[19]	WANG Zheng-jia, HUANG You-jun, XIA Guo-hua, ZHENG Bing-song, JIN Song-heng, HUANG Jian-qin. Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis . Journal of Zhejiang A&F University, 2008, 25(4): 427-430.
[20]	HUANG Jian-qin, FANG Wei, DING Yu-long, HE Fu-ji. Analysis on factors effecting survival rate in Carya cathayensis grafting . Journal of Zhejiang A&F University, 2002, 19(3): 227-230.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(2167) PDF downloads(531) Cited by()

Proportional views

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. Agricultural Office of Toutuo People’s Government, Huangyan District, Huangyan 318026, Zhejiang, China;

3. Jinhua Institute of Agricultural Sciences, Jinhua 321017, Zhejiang, China

Received Date: 2014-06-12

Rev Recd Date: 2014-08-24

Publish Date: 2015-04-20

Key words:

Abstract: Carya cathayensis is an important oil species with dry nuts that are high oil content, for which there must be some special oil-synthesizing mechanism. In order to explore the mechanism, random cloning and sequencing of cDNAs from a fatty acid metabolism-associated cDNA library, sequence assembly and annotation, Gene Ontology （GO）- and Arabidopsis thaliana-associated MIPS Functional Catalogue （FunCat）-based classification of assembled sequences, and protein analysis of fatty acid metabolism-associated cDNA sequences were performed. A total of 1 010 expressed sequence tags （ESTs） was obtained, based on which 188 unigenes were assembled. There were 92 contigs and 96 singlets among the unigenes, which were classified into categories of cellular component, molecular function, and biological process based on classification of GO and found to overlap one another in functions. But against MIPS Functional Catalogue in Arabidopsis thaliana, these unigenes were classified into nine categories and 143 full-length cDNAs were obtained, from which 14 cDNA sequences were associated with metabolism of fatty acids. It had been found through proteomic analysis that 12 full-length fatty acid metabolism-related cDNAs had a phosphorylation site; they were all non-secretory proteins with no transmembrane domain and signal peptide except oleosins, belonging to certain protein family; and oleosins had different transmembrane domains and a signal peptide, belonging to a super oleosin family. ［Ch, 4 tab. 35 ref.］

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.02.009

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.02.009

HTML

Catalog

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.02.009

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.02.009

HTML

Catalog

Export File

Citation

Format

Content