Analysis of ESTs associated with oil metabolism in seeds of <em>Carya cathayensis</em>

HUANG Yinzhi; ZENG Yanru; ZHOU Qin; XIA Guohua; HUANG Youjun

doi:10.11833/j.issn.2095-0756.2015.02.009

Volume 32 Issue 2

Mar. 2015

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HUANG Yinzhi, ZENG Yanru, ZHOU Qin, XIA Guohua, HUANG Youjun. Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009

Citation:

HUANG Yinzhi, ZENG Yanru, ZHOU Qin, XIA Guohua, HUANG Youjun. Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(2): 229-236. doi: 10.11833/j.issn.2095-0756.2015.02.009

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.02.009

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
2.
Agricultural Office of Toutuo People’s Government, Huangyan District, Huangyan 318026, Zhejiang, China;
3.
Jinhua Institute of Agricultural Sciences, Jinhua 321017, Zhejiang, China

Received Date: 2014-06-12
Rev Recd Date: 2014-08-24
Publish Date: 2015-04-20

Abstract

Carya cathayensis is an important oil species with dry nuts that are high oil content, for which there must be some special oil-synthesizing mechanism. In order to explore the mechanism, random cloning and sequencing of cDNAs from a fatty acid metabolism-associated cDNA library, sequence assembly and annotation, Gene Ontology （GO）- and Arabidopsis thaliana-associated MIPS Functional Catalogue （FunCat）-based classification of assembled sequences, and protein analysis of fatty acid metabolism-associated cDNA sequences were performed. A total of 1 010 expressed sequence tags （ESTs） was obtained, based on which 188 unigenes were assembled. There were 92 contigs and 96 singlets among the unigenes, which were classified into categories of cellular component, molecular function, and biological process based on classification of GO and found to overlap one another in functions. But against MIPS Functional Catalogue in Arabidopsis thaliana, these unigenes were classified into nine categories and 143 full-length cDNAs were obtained, from which 14 cDNA sequences were associated with metabolism of fatty acids. It had been found through proteomic analysis that 12 full-length fatty acid metabolism-related cDNAs had a phosphorylation site; they were all non-secretory proteins with no transmembrane domain and signal peptide except oleosins, belonging to certain protein family; and oleosins had different transmembrane domains and a signal peptide, belonging to a super oleosin family. ［Ch, 4 tab. 35 ref.］
- cash forestry,
- Carya cathayensis,
- expressed sequence tag,
- functional classification,
- fatty acid metabolism,
- protein analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. Agricultural Office of Toutuo People’s Government, Huangyan District, Huangyan 318026, Zhejiang, China;

3. Jinhua Institute of Agricultural Sciences, Jinhua 321017, Zhejiang, China

Received Date: 2014-06-12

Rev Recd Date: 2014-08-24

Publish Date: 2015-04-20

Key words:

Abstract: Carya cathayensis is an important oil species with dry nuts that are high oil content, for which there must be some special oil-synthesizing mechanism. In order to explore the mechanism, random cloning and sequencing of cDNAs from a fatty acid metabolism-associated cDNA library, sequence assembly and annotation, Gene Ontology （GO）- and Arabidopsis thaliana-associated MIPS Functional Catalogue （FunCat）-based classification of assembled sequences, and protein analysis of fatty acid metabolism-associated cDNA sequences were performed. A total of 1 010 expressed sequence tags （ESTs） was obtained, based on which 188 unigenes were assembled. There were 92 contigs and 96 singlets among the unigenes, which were classified into categories of cellular component, molecular function, and biological process based on classification of GO and found to overlap one another in functions. But against MIPS Functional Catalogue in Arabidopsis thaliana, these unigenes were classified into nine categories and 143 full-length cDNAs were obtained, from which 14 cDNA sequences were associated with metabolism of fatty acids. It had been found through proteomic analysis that 12 full-length fatty acid metabolism-related cDNAs had a phosphorylation site; they were all non-secretory proteins with no transmembrane domain and signal peptide except oleosins, belonging to certain protein family; and oleosins had different transmembrane domains and a signal peptide, belonging to a super oleosin family. ［Ch, 4 tab. 35 ref.］

Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.02.009

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Analysis of ESTs associated with oil metabolism in seeds of Carya cathayensis

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