The rDNA ITS diversity of mycorrhizal fungi with <em>Cypripedium flavum</em>

MIAO Fujun; JIANG Hong; WANG Hongqiu; YUAN Xiaolong; CHEN Jian; YANG Yuming; WANG Juan

doi:10.11833/j.issn.2095-0756.2015.05.024

Volume 32 Issue 5

Sep. 2015

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MIAO Fujun, JIANG Hong, WANG Hongqiu, YUAN Xiaolong, CHEN Jian, YANG Yuming, WANG Juan. The rDNA ITS diversity of mycorrhizal fungi with Cypripedium flavum[J]. Journal of Zhejiang A&F University, 2015, 32(5): 815-820. doi: 10.11833/j.issn.2095-0756.2015.05.024

Citation:

MIAO Fujun, JIANG Hong, WANG Hongqiu, YUAN Xiaolong, CHEN Jian, YANG Yuming, WANG Juan. The rDNA ITS diversity of mycorrhizal fungi with Cypripedium flavum[J]. Journal of Zhejiang A&F University, 2015, 32(5): 815-820. doi: 10.11833/j.issn.2095-0756.2015.05.024

The rDNA ITS diversity of mycorrhizal fungi with Cypripedium flavum

doi: 10.11833/j.issn.2095-0756.2015.05.024

1.
Key Laboratory for Conservation of Rare, Endangered & Endemic Forest Plants, State Forestry Administration, Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650201, Yunnan, China;
2.
School of Forestry, Southwest Forestry University, Kunming 650224, Yunnan, China

Received Date: 2014-12-08
Rev Recd Date: 2015-01-22
Publish Date: 2015-10-20

Abstract

Cypripedium plants, which are endangered by over-harvesting due to their high ornamental and medicinal value, use mycorrhizal fungi to guarantee cultivation and conservation. In order to explore the diversity of mycorrhizal fungi, the rDNA internal transcribed spacer （ITS） sequences from mycorrhizal fungi of four C. flavum populations （Baishui River, Shika Snow Mountain, Tiansheng Bridge, and Napa Village） were amplified by nested polymerase chain reaction （PCR） methods. Results showed a total of 336 distinct ITS-taxa of C. flavum obtained from clones of mycorrhizal fungi with populations from Baishui River （93）, Shika Snow Mountain （90）, Tiansheng Bridge （103）, and Napa Village （80）. Blast results of rDNA ITS sequences showed a rich diversity in mycorrhizal fungi systems with mycorrhizae belonging to Tulasnella, Corticium, Epulorhiza, and Rhizoctoia. Tulasnella fungi probably had host-specificity and growth-promoting effects on C. flavum. These results may provide a scientific basis for further conservation work of C. flavum and fungal manure development.［Ch, 1 fig. 3 tab. 24 ref.］
- microbiology,
- Cypripedium flavum,
- mycorrhizal fungi,
- rDNA ITS,
- Tulasnella,
- diversity

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The rDNA ITS diversity of mycorrhizal fungi with Cypripedium flavum

1. Key Laboratory for Conservation of Rare, Endangered & Endemic Forest Plants, State Forestry Administration, Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650201, Yunnan, China;

2. School of Forestry, Southwest Forestry University, Kunming 650224, Yunnan, China

Received Date: 2014-12-08

Rev Recd Date: 2015-01-22

Publish Date: 2015-10-20

Key words:

Abstract: Cypripedium plants, which are endangered by over-harvesting due to their high ornamental and medicinal value, use mycorrhizal fungi to guarantee cultivation and conservation. In order to explore the diversity of mycorrhizal fungi, the rDNA internal transcribed spacer （ITS） sequences from mycorrhizal fungi of four C. flavum populations （Baishui River, Shika Snow Mountain, Tiansheng Bridge, and Napa Village） were amplified by nested polymerase chain reaction （PCR） methods. Results showed a total of 336 distinct ITS-taxa of C. flavum obtained from clones of mycorrhizal fungi with populations from Baishui River （93）, Shika Snow Mountain （90）, Tiansheng Bridge （103）, and Napa Village （80）. Blast results of rDNA ITS sequences showed a rich diversity in mycorrhizal fungi systems with mycorrhizae belonging to Tulasnella, Corticium, Epulorhiza, and Rhizoctoia. Tulasnella fungi probably had host-specificity and growth-promoting effects on C. flavum. These results may provide a scientific basis for further conservation work of C. flavum and fungal manure development.［Ch, 1 fig. 3 tab. 24 ref.］

The rDNA ITS diversity of mycorrhizal fungi with Cypripedium flavum

doi: 10.11833/j.issn.2095-0756.2015.05.024

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