Selection of wheat having resistance to yellow mosaic virus and screen out SSR molecular markers having polymorphism between resistant and sensitive parents

WEI Wei; LI Junmin; SUN Liying; RONG Junkang; ZHOU Wei

doi:10.11833/j.issn.2095-0756.2016.01.010

Volume 33 Issue 1

Jan. 2016

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WEI Wei, LI Junmin, SUN Liying, RONG Junkang, ZHOU Wei. Selection of wheat having resistance to yellow mosaic virus and screen out SSR molecular markers having polymorphism between resistant and sensitive parents[J]. Journal of Zhejiang A&F University, 2016, 33(1): 71-79. doi: 10.11833/j.issn.2095-0756.2016.01.010

Citation:

WEI Wei, LI Junmin, SUN Liying, RONG Junkang, ZHOU Wei. Selection of wheat having resistance to yellow mosaic virus and screen out SSR molecular markers having polymorphism between resistant and sensitive parents[J]. Journal of Zhejiang A&F University, 2016, 33(1): 71-79. doi: 10.11833/j.issn.2095-0756.2016.01.010

Selection of wheat having resistance to yellow mosaic virus and screen out SSR molecular markers having polymorphism between resistant and sensitive parents

doi: 10.11833/j.issn.2095-0756.2016.01.010

1.
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
2.
Key Laboratory of Pest and Disease Control Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China;
3.
College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China

Funds:

浙江省科技创新活动计划（新苗人才计划）项目（2014R412048）;浙江省植物有害生物防控重点实验室开放基金资助项目（2010DS700124-KF1103）;浙江省旱粮农业新品种选育重大科技专项（2012C12902-2-6）;浙江省科技创新团队项目（2011R50026-23）;浙江省自然科学基金资助项目（LQ12C13003）

Received Date: 2015-03-27
Rev Recd Date: 2015-06-01
Publish Date: 2016-02-20

Abstract

Wheat yellow mosaic virus （WYMV）, one of the most devastating diseases of cereals, also called wheat spindle streak mosaic virus （WSSMV）, has different effects on different cultivars of wheat. Breeding and growing resistant cultivars is one of the most effective method to defense WYMV. Selection of suitable parents for the formation of mapping population for QTLs mapping involved in WYMV is a basic work to breed new WYMV resistant cultivars. In this study, 527 different cultivars / lines of wheat from different districts at home and abroad were introduced and identified in disease plots with three different experimental plots and three replications each plot. Then polymorphic simple sequence repeats （SSRs） markers were screened out between resistant and sensitive parents which had a larger planting area than other wheat cultivars. Results showed that only ten of the 527 candidate materials had a relatively more stable WYMV disease resistance. Three WYMV resistant wheat cultivars out of ten resistant materials including Fengkang 1Shijiazhuang 72and Zhengmai 9023and two sensitive wheat cultivars with a wider planting area, Yangmai 158 and Yannong 22, were selected for further comparison. Higher polymorphic rates were obtained with the parental group of Fengkang 1 and Yangmai 158 （64.0%） and the parental group of Shijiazhuang 72 and Yangmai 158（63.3%） than other groups. Therefore, using WYMV resistant cultivars Fengkang 1 and Shijiazhuang 72, along with WYMV sensitive parent Yangmai 158 to form mapping populations would be more opportune for fine mapping of quantitative trait locus （QTLs） involved in WYMV resistance and map-based gene cloning. ［Ch, 2 fig. 3 tab. 17 ref.］
- plant protection,
- Triticum aestivum,
- molecular marker,
- yellow mosaic virus,
- resistance

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Selection of wheat having resistance to yellow mosaic virus and screen out SSR molecular markers having polymorphism between resistant and sensitive parents

1. The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. Key Laboratory of Pest and Disease Control Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China;

3. College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China

Funds:

Received Date: 2015-03-27

Rev Recd Date: 2015-06-01

Publish Date: 2016-02-20

Key words:

Abstract: Wheat yellow mosaic virus （WYMV）, one of the most devastating diseases of cereals, also called wheat spindle streak mosaic virus （WSSMV）, has different effects on different cultivars of wheat. Breeding and growing resistant cultivars is one of the most effective method to defense WYMV. Selection of suitable parents for the formation of mapping population for QTLs mapping involved in WYMV is a basic work to breed new WYMV resistant cultivars. In this study, 527 different cultivars / lines of wheat from different districts at home and abroad were introduced and identified in disease plots with three different experimental plots and three replications each plot. Then polymorphic simple sequence repeats （SSRs） markers were screened out between resistant and sensitive parents which had a larger planting area than other wheat cultivars. Results showed that only ten of the 527 candidate materials had a relatively more stable WYMV disease resistance. Three WYMV resistant wheat cultivars out of ten resistant materials including Fengkang 1Shijiazhuang 72and Zhengmai 9023and two sensitive wheat cultivars with a wider planting area, Yangmai 158 and Yannong 22, were selected for further comparison. Higher polymorphic rates were obtained with the parental group of Fengkang 1 and Yangmai 158 （64.0%） and the parental group of Shijiazhuang 72 and Yangmai 158（63.3%） than other groups. Therefore, using WYMV resistant cultivars Fengkang 1 and Shijiazhuang 72, along with WYMV sensitive parent Yangmai 158 to form mapping populations would be more opportune for fine mapping of quantitative trait locus （QTLs） involved in WYMV resistance and map-based gene cloning. ［Ch, 2 fig. 3 tab. 17 ref.］

Selection of wheat having resistance to yellow mosaic virus and screen out SSR molecular markers having polymorphism between resistant and sensitive parents

doi: 10.11833/j.issn.2095-0756.2016.01.010

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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