Tissue culture and rapid propagation for stems of <em>Cinnamomum camphora</em>

YE Runyan; TONG Zaikang; ZHANG Junhong; ZHU Yuqiu

doi:10.11833/j.issn.2095-0756.2016.01.024

Volume 33 Issue 1

Jan. 2016

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YE Runyan, TONG Zaikang, ZHANG Junhong, et al. Tissue culture and rapid propagation for stems of Cinnamomum camphora[J]. Journal of Zhejiang A&F University, 2016, 33(1): 177-182. DOI: 10.11833/j.issn.2095-0756.2016.01.024

Citation:

YE Runyan, TONG Zaikang, ZHANG Junhong, et al. Tissue culture and rapid propagation for stems of Cinnamomum camphora[J]. Journal of Zhejiang A&F University, 2016, 33(1): 177-182. DOI: 10.11833/j.issn.2095-0756.2016.01.024

Tissue culture and rapid propagation for stems of Cinnamomum camphora

DOI: 10.11833/j.issn.2095-0756.2016.01.024

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

浙江省林木种苗产业创新团队资助项目（2009R50035）;浙江省竹木育种重大专项（2012C12908?鄄7）;浙江农林大学研究生科研创新基金资助项目（3122013240236）

Received Date: 2015-01-29
Rev Recd Date: 2015-06-04
Publish Date: 2016-02-20

Abstract

To study the effect of naphthalene-acetic acid （NAA） and 6-benzylaminopurine 6-BA on the induction and multiplication of multiple shoots for Cinnamomum camphora during the process of primary culture and subculture, stems with axillary buds which sprouted at the beginning of spring were used as explants. To select the optimum medium for subculture, an orthogonal experiment was designed, and an extreme difference analysis and variance analysis （ANOVA） was used. Results showed that the optimal medium for inducing axillary buds was Murashige and Skoog （MS） + 1.00 mgL－1 6-BA + （0.01－0.10 mgL－1） NAA. The orthogonal experiment and ANOVA showed that the best medium for formation of multiple shoots was MS + 1.00 mgL－1 6-BA + 0.10 mgL－1 NAA, and the optimum medium for proliferation of multiple shoots was MS + 0.50－1.00 mg L－1 6-BA. After the aseptic seedling was transferred to the optimum rooting medium （1/2MS + 1.50 mg L－1 NAA）, the rooting rate reached above 90%. After 15－20 d of hardening, seedlings were transplanted to a mixed matrix with equal vermiculite and peat where the survival rate was greater than 95%. Thus, an efficient breeding technology for C. camphora was studied laying a technical foundation for industrialization of improved varieties. ［Ch, 2 fig. 5 tab. 13 ref.］
- silviculture,
- Cinnamomum camphora,
- clustered shoots,
- asexual rapid propagation,
- tissue culture

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Tissue culture and rapid propagation for stems of Cinnamomum camphora

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

浙江省林木种苗产业创新团队资助项目（2009R50035）;浙江省竹木育种重大专项（2012C12908?鄄7）;浙江农林大学研究生科研创新基金资助项目（3122013240236）

Received Date: 2015-01-29

Rev Recd Date: 2015-06-04

Publish Date: 2016-02-20

Key words:

Abstract: To study the effect of naphthalene-acetic acid （NAA） and 6-benzylaminopurine 6-BA on the induction and multiplication of multiple shoots for Cinnamomum camphora during the process of primary culture and subculture, stems with axillary buds which sprouted at the beginning of spring were used as explants. To select the optimum medium for subculture, an orthogonal experiment was designed, and an extreme difference analysis and variance analysis （ANOVA） was used. Results showed that the optimal medium for inducing axillary buds was Murashige and Skoog （MS） + 1.00 mgL－1 6-BA + （0.01－0.10 mgL－1） NAA. The orthogonal experiment and ANOVA showed that the best medium for formation of multiple shoots was MS + 1.00 mgL－1 6-BA + 0.10 mgL－1 NAA, and the optimum medium for proliferation of multiple shoots was MS + 0.50－1.00 mg L－1 6-BA. After the aseptic seedling was transferred to the optimum rooting medium （1/2MS + 1.50 mg L－1 NAA）, the rooting rate reached above 90%. After 15－20 d of hardening, seedlings were transplanted to a mixed matrix with equal vermiculite and peat where the survival rate was greater than 95%. Thus, an efficient breeding technology for C. camphora was studied laying a technical foundation for industrialization of improved varieties. ［Ch, 2 fig. 5 tab. 13 ref.］

Tissue culture and rapid propagation for stems of Cinnamomum camphora

DOI: 10.11833/j.issn.2095-0756.2016.01.024

Abstract

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通讯作者: 陈斌, bchen63@163.com

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Tissue culture and rapid propagation for stems of Cinnamomum camphora

doi: 10.11833/j.issn.2095-0756.2016.01.024

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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Tissue culture and rapid propagation for stems of Cinnamomum camphora

DOI: 10.11833/j.issn.2095-0756.2016.01.024

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Tissue culture and rapid propagation for stems of Cinnamomum camphora

doi: 10.11833/j.issn.2095-0756.2016.01.024

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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