Identification and characterization of salt-responsive transcription factors in roots of southern type alfalfa

MA Jin; ZHENG Gang; PEI Cuiming; ZHANG Zhenya

doi:10.11833/j.issn.2095-0756.2016.02.003

Volume 33 Issue 2

Mar. 2016

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MA Jin, ZHENG Gang, PEI Cuiming, ZHANG Zhenya. Identification and characterization of salt-responsive transcription factors in roots of southern type alfalfa[J]. Journal of Zhejiang A&F University, 2016, 33(2): 201-208. doi: 10.11833/j.issn.2095-0756.2016.02.003

Citation:

MA Jin, ZHENG Gang, PEI Cuiming, ZHANG Zhenya. Identification and characterization of salt-responsive transcription factors in roots of southern type alfalfa[J]. Journal of Zhejiang A&F University, 2016, 33(2): 201-208. doi: 10.11833/j.issn.2095-0756.2016.02.003

Identification and characterization of salt-responsive transcription factors in roots of southern type alfalfa

doi: 10.11833/j.issn.2095-0756.2016.02.003

1.
School of Landscape Architecture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

国家自然科学基金资助项目（31272494）;浙江省自然科学基金资助项目（LY16C170003）

Received Date: 2015-04-25
Rev Recd Date: 2015-07-19
Publish Date: 2016-04-20

Abstract

Transcription factors （TFs）, which can regulate downstream gene expression, play an important role in plant stress responses. In order to investigate the molecular mechanism of salt tolerance, the TFs of southern type alfalfa, in this study with 250 mmolL－1 NaCl stress, Illumina RNA-sequencing was performed to evaluate the expression spectrum of transcription factors in roots of the southern alfalfa cultivar Millennium. Then to verify the expression of four randomly selected genes, Quantitative Reverse-Transcriptase Polymerase Chain Reaction （qRT-PCR）（three repeated） was used. Results showed 31 907 differentially expressed genes, 2 758 of which showed a difference of over two fold. Among these genes, 199 transcription factors belonging to 38 TF families were up-regulated and 95 were down-regulated. Genes from the MYB family were observed most, followed by AP2-EREBP, bHLH, WRKY, NAC, and GRAS. The qRT-PCR assay of four randomly selected genes confirmed the results of RNA-Seq analysis. In addition, candidate genes such as MsERF-2b，MsbHLH, MsbZIP, MsC2H2, MsGRAS, MsNAC, MsMGT-3a, and MsWRKY that may be involved in salt stress responses were identified. This study indicated that multiple TF families were involved in salt stress responses in the root of southern alfalfa types, and it provided new information for further study of the mechanism of a plants response to salt stress.［Ch, 3 fig. 3 tab. 36 ref.］
- plant breeding,
- southern type alfalfa,
- transcription factor,
- root transcriptome,
- salt stress

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Identification and characterization of salt-responsive transcription factors in roots of southern type alfalfa

1. School of Landscape Architecture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

国家自然科学基金资助项目（31272494）;浙江省自然科学基金资助项目（LY16C170003）

Received Date: 2015-04-25

Rev Recd Date: 2015-07-19

Publish Date: 2016-04-20

Key words:

Abstract: Transcription factors （TFs）, which can regulate downstream gene expression, play an important role in plant stress responses. In order to investigate the molecular mechanism of salt tolerance, the TFs of southern type alfalfa, in this study with 250 mmolL－1 NaCl stress, Illumina RNA-sequencing was performed to evaluate the expression spectrum of transcription factors in roots of the southern alfalfa cultivar Millennium. Then to verify the expression of four randomly selected genes, Quantitative Reverse-Transcriptase Polymerase Chain Reaction （qRT-PCR）（three repeated） was used. Results showed 31 907 differentially expressed genes, 2 758 of which showed a difference of over two fold. Among these genes, 199 transcription factors belonging to 38 TF families were up-regulated and 95 were down-regulated. Genes from the MYB family were observed most, followed by AP2-EREBP, bHLH, WRKY, NAC, and GRAS. The qRT-PCR assay of four randomly selected genes confirmed the results of RNA-Seq analysis. In addition, candidate genes such as MsERF-2b，MsbHLH, MsbZIP, MsC2H2, MsGRAS, MsNAC, MsMGT-3a, and MsWRKY that may be involved in salt stress responses were identified. This study indicated that multiple TF families were involved in salt stress responses in the root of southern alfalfa types, and it provided new information for further study of the mechanism of a plants response to salt stress.［Ch, 3 fig. 3 tab. 36 ref.］

Identification and characterization of salt-responsive transcription factors in roots of southern type alfalfa

doi: 10.11833/j.issn.2095-0756.2016.02.003

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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