Leave source from callus induction and flavonoid content in callus from different tissues of five Ginkgo biloba cultivars

Key words: 
• botany /
• Ginkgo biloba /
• leaves area /
• callus /
• flavonoids

Abstract: The objectives of this study with Ginkgo biloba were to establish a callus culture system for leaf sources and to determine the flavonoid contentin callus fromdifferentgingko tissues.Five elite cultivars(Number44, 45,28, 79 and 53)with mediums of N6, MS(MurashigeSkoog medium), improved MS and DCR(Douglas-fir cotyledon revised medium), and growth supplements of NAA(naphthalene acetic acid), 6-BA(6-benzylaminopurine), and KT(kinetin)were tested with roots, leaves, cotyledons, and stems as cultured explants. The induction rate, growth, and flavonoid contentin the callusfromdifferentcultivars and explantswere canpared. Results indicated thata supplementof 0.1- 0.5 mgL- 1 ofNAAand 0.5- 1.0 mgL- 1 of 6-BAin the mediumfavored leaf size(P 0.01). The induction rates of leaf callus in cultivars` 28' and` 79' were significantly higher(P 0.01)than the other cultivars.MediumN6 was better for inducing callus than MS, but N6 was not better for a subculture. The 6-BA supplement in a medium more readily(P 0.01) induced callus thanKT.Also, the flavonoid content in the callus of cultivars`28' and`79' was higher(P 0.01) than the other cultivars.Forall cultivars,flavonoid contentin the callusfromdifferentexplantswas root leaf cotyledon stem.[Ch, 2 fig. 4 tab. 18 ref.]