珍珠黄杨春季扦插生根性状差异及内源激素变化

黄焱; 季孔庶; 方彦; 翟锦如

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珍珠黄杨春季扦插生根性状差异及内源激素变化

黄焱 , 季孔庶 , 方彦 , 翟锦如

文章导航 > 浙江农林大学学报 > 2007 > 24(3): 284-289

黄焱, 季孔庶, 方彦, 翟锦如. 珍珠黄杨春季扦插生根性状差异及内源激素变化[J]. 浙江农林大学学报, 2007, 24(3): 284-289.

引用本文:

黄焱, 季孔庶, 方彦, 翟锦如. 珍珠黄杨春季扦插生根性状差异及内源激素变化[J]. 浙江农林大学学报, 2007, 24(3): 284-289.

HUANG Yan, JI Kong-shu, FANG Yan, ZHAI Jin-ru. Rooting characteristics and endogenous hormone levels in Buxus sinica var. parvifolia during spring cutting[J]. Journal of Zhejiang A&F University, 2007, 24(3): 284-289.

Citation:

HUANG Yan, JI Kong-shu, FANG Yan, ZHAI Jin-ru. Rooting characteristics and endogenous hormone levels in Buxus sinica var. parvifolia during spring cutting[J]. Journal of Zhejiang A&F University, 2007, 24(3): 284-289.

珍珠黄杨春季扦插生根性状差异及内源激素变化

黄焱¹,
季孔庶^1, ,,
方彦²,
翟锦如³

1.
南京林业大学国家林业局林木遗传和基因工程重点实验室,江苏南京210037;
2.
南京森林公安高等专科学校科技处,江苏南京210046;
3.
好雨园景有限公司,江苏如皋226553

基金项目:

国家自然科学基金资助项目(30070633)

详细信息

作者简介: 黄焱,博士研究生,从事园林植物遗传育种研究。

通信作者: 季孔庶,教授,博士,从事林木及园林植物遗传育种研究。

中图分类号: S718.43

Rooting characteristics and endogenous hormone levels in Buxus sinica var. parvifolia during spring cutting

1.
Key Laboratory of Forest Genetics and Gene Engineering of State Administration of Forestry, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;
2.
Science and Technology Department, Nanjing Forest Police College, Nanjing 210046, Jiangsu, China;
3.
Haoyu Landscape Co., Ltd, Rugao 226553, Jiangsu, China

Funds:

国家自然科学基金资助项目(30070633)

摘要: 为了研究珍珠黄杨Buxus sinica var. parvifolia扦插生根的相关机制,通过酶联免疫吸附分析(ELISA)技术对春季扦插生根过程中各品种的吲哚乙酸(IAA),脱落酸(ABA),异戊烯基核苷(iPA)和赤霉素4(GA4)质量分数作进程研究,并对其生根性状结果作统计分析。研究发现:在愈合组织产生前后及根突出表皮时, IAA、ABA和GA4质量分数的变化曲线在不同品种处理之间存在差异, 3号和5号品种在ABT6处理后扦插生根能力有了提高。结果表明,易生根品种的激素质量分数在生根关键时期更利于插穗愈合组织的产生以及根原基的形成与分化。图4表2参18
- 植物学 /
- 珍珠黄杨 /
- 品种 /
- 内源激素 /
- 扦插 /
- 愈合组织
Abstract: Buxus sinica var. parvifolia(littleaf box) is one of the woody evergreen plant species that has high commercial value in the floriculture market. Efforts to improve propagation successwill be essential in development of the species for commercial production and in satisfy the markets needs. The purpose of this research is to study the rooting mechanism of Buxus sinica var. parvifolia cutting. Seven cultivars were chosen, including five cultivars of littleleaf box and two of Buxus sinica(Chinese box) to compare the rooting potential. Enzyme-linked immunosorbent assay(ELISA) technology was used to measure the levels of indoleacetic acid(IAA), abscisic acid(ABA), isopentenyladenosine(iPA), and gibberellin4(GA4) in the rooting zones, and the rooting characterswere also observed in spring stem cutting. The results showed that around the time of callus forming and the root projecting epidermis, the levels of IAA, ABA and GA4were different in seven cultivars and in different treatments. And the rooting ability of cultivarNo.3 and No.5 treated byABT6 were improved. The high levels of endogenous hormone in the easy-to-rootcultivars in crucial rooting periodwasbeneficial to callusgeneration and the root anlage formation and differentiation.[Ch, 4 fig. 2 tab. 18 ref.]
- botany /
- Buxus sinica var. parvifolia /
- cultivar /
- endogenous hormone /
- cutting /
- callus

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珍珠黄杨春季扦插生根性状差异及内源激素变化

1. 南京林业大学国家林业局林木遗传和基因工程重点实验室,江苏南京210037;

2. 南京森林公安高等专科学校科技处,江苏南京210046;

3. 好雨园景有限公司,江苏如皋226553

基金项目:

国家自然科学基金资助项目(30070633)

作者简介:
黄焱,博士研究生,从事园林植物遗传育种研究。

通信作者: 季孔庶,教授,博士,从事林木及园林植物遗传育种研究。

收稿日期: 2006-10-13
修回日期: 2006-12-18
刊出日期: 2007-06-20

中图分类号: S718.43

关键词:

摘要: 为了研究珍珠黄杨Buxus sinica var. parvifolia扦插生根的相关机制,通过酶联免疫吸附分析(ELISA)技术对春季扦插生根过程中各品种的吲哚乙酸(IAA),脱落酸(ABA),异戊烯基核苷(iPA)和赤霉素4(GA4)质量分数作进程研究,并对其生根性状结果作统计分析。研究发现:在愈合组织产生前后及根突出表皮时, IAA、ABA和GA4质量分数的变化曲线在不同品种处理之间存在差异, 3号和5号品种在ABT6处理后扦插生根能力有了提高。结果表明,易生根品种的激素质量分数在生根关键时期更利于插穗愈合组织的产生以及根原基的形成与分化。图4表2参18

English Abstract

Rooting characteristics and endogenous hormone levels in Buxus sinica var. parvifolia during spring cutting

1. Key Laboratory of Forest Genetics and Gene Engineering of State Administration of Forestry, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;

2. Science and Technology Department, Nanjing Forest Police College, Nanjing 210046, Jiangsu, China;

3. Haoyu Landscape Co., Ltd, Rugao 226553, Jiangsu, China

Funds:

国家自然科学基金资助项目(30070633)

Received Date: 2006-10-13
Rev Recd Date: 2006-12-18
Publish Date: 2007-06-20

Keywords:

Abstract: Buxus sinica var. parvifolia(littleaf box) is one of the woody evergreen plant species that has high commercial value in the floriculture market. Efforts to improve propagation successwill be essential in development of the species for commercial production and in satisfy the markets needs. The purpose of this research is to study the rooting mechanism of Buxus sinica var. parvifolia cutting. Seven cultivars were chosen, including five cultivars of littleleaf box and two of Buxus sinica(Chinese box) to compare the rooting potential. Enzyme-linked immunosorbent assay(ELISA) technology was used to measure the levels of indoleacetic acid(IAA), abscisic acid(ABA), isopentenyladenosine(iPA), and gibberellin4(GA4) in the rooting zones, and the rooting characterswere also observed in spring stem cutting. The results showed that around the time of callus forming and the root projecting epidermis, the levels of IAA, ABA and GA4were different in seven cultivars and in different treatments. And the rooting ability of cultivarNo.3 and No.5 treated byABT6 were improved. The high levels of endogenous hormone in the easy-to-rootcultivars in crucial rooting periodwasbeneficial to callusgeneration and the root anlage formation and differentiation.[Ch, 4 fig. 2 tab. 18 ref.]

黄焱, 季孔庶, 方彦, 翟锦如. 珍珠黄杨春季扦插生根性状差异及内源激素变化[J]. 浙江农林大学学报, 2007, 24(3): 284-289.

引用本文:

黄焱, 季孔庶, 方彦, 翟锦如. 珍珠黄杨春季扦插生根性状差异及内源激素变化[J]. 浙江农林大学学报, 2007, 24(3): 284-289.