Optimal reaction system for random amplified polymorphic DNA with endangered <em>Emmenopterys henryi</em>

YANG Jing-yuan; XIONG Dan; LIANG Hong-wei; WANG Yu-bing; LIAOMing-ya; CHEN Fa-ju

Volume 24 Issue 3

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(3): 279-283

YANG Jing-yuan, XIONG Dan, LIANG Hong-wei, WANG Yu-bing, LIAOMing-ya, CHEN Fa-ju. Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi[J]. Journal of Zhejiang A&F University, 2007, 24(3): 279-283.

Citation:

YANG Jing-yuan, XIONG Dan, LIANG Hong-wei, WANG Yu-bing, LIAOMing-ya, CHEN Fa-ju. Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi[J]. Journal of Zhejiang A&F University, 2007, 24(3): 279-283.

Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi

1.
Administration of Shennongjia Nature Reserve, Shennongjia 442421, Hubei, China;
2.
Biotechnology ResearchCenter-Hubei Key Laboratory of Natural Products Use and Research, Three Gorges University, Yichang 443002, Hubei, China

Funds:

湖北省宜昌市科学技术重点攻关项目(A06209)

Received Date: 2006-09-18
Rev Recd Date: 2006-12-12
Publish Date: 2007-06-20

Abstract

Genome DNA from leaves of the Emmenopterys henryi tree(Henry emmenopterys) in the Shennongjia area of Hubei Province, China, was extracted to optimize its random amplified polymorphic DNA(RAPD) reaction conditions, which included concentrations of DNA polymerase, primers, Mg2+,dNTP, and DNA.Resultsindicated that genome DNA of E. henryi had good amplification with the following conditions: 25L solutionwith 1.33 10- 3 katL- 1 Taq DNA polymerase, 0.5molL- 1 random primer, 2.6 mmolL- 1 Mg2+,220molL- 1 dNTP, and 4.40 mgL- 1 genomic DNA. The polymerase chain reaction(PCR) amplification programwas as follows: predenaturing at 94℃ for5 min, followed by 40 cycles of denaturing at 94℃ for1 min,annealing at 43℃ for 1 min, extension at 72℃ for 2 min, and final extension at 72℃ for 8 min. Such a system and reaction programs could achieve comparatively stable amplification.[Ch, 6 fig. 1 tab. 8 ref.]
- botany,
- Emmenopterys henryi(Henry emmenoterys),
- genomic DNA,
- randomamplified polymorphic DNA(RAPD)

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi

1. Administration of Shennongjia Nature Reserve, Shennongjia 442421, Hubei, China;

2. Biotechnology ResearchCenter-Hubei Key Laboratory of Natural Products Use and Research, Three Gorges University, Yichang 443002, Hubei, China

Funds:

湖北省宜昌市科学技术重点攻关项目(A06209)

Received Date: 2006-09-18

Rev Recd Date: 2006-12-12

Publish Date: 2007-06-20

Key words:

botany /
Emmenopterys henryi(Henry emmenoterys) /
genomic DNA /
randomamplified polymorphic DNA(RAPD)

Abstract: Genome DNA from leaves of the Emmenopterys henryi tree(Henry emmenopterys) in the Shennongjia area of Hubei Province, China, was extracted to optimize its random amplified polymorphic DNA(RAPD) reaction conditions, which included concentrations of DNA polymerase, primers, Mg2+,dNTP, and DNA.Resultsindicated that genome DNA of E. henryi had good amplification with the following conditions: 25L solutionwith 1.33 10- 3 katL- 1 Taq DNA polymerase, 0.5molL- 1 random primer, 2.6 mmolL- 1 Mg2+,220molL- 1 dNTP, and 4.40 mgL- 1 genomic DNA. The polymerase chain reaction(PCR) amplification programwas as follows: predenaturing at 94℃ for5 min, followed by 40 cycles of denaturing at 94℃ for1 min,annealing at 43℃ for 1 min, extension at 72℃ for 2 min, and final extension at 72℃ for 8 min. Such a system and reaction programs could achieve comparatively stable amplification.[Ch, 6 fig. 1 tab. 8 ref.]

Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi

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通讯作者: 陈斌, bchen63@163.com

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Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi

1. Administration of Shennongjia Nature Reserve, Shennongjia 442421, Hubei, China;

2. Biotechnology ResearchCenter-Hubei Key Laboratory of Natural Products Use and Research, Three Gorges University, Yichang 443002, Hubei, China

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Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi

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Optimal reaction system for random amplified polymorphic DNA with endangered Emmenopterys henryi

1. Administration of Shennongjia Nature Reserve, Shennongjia 442421, Hubei, China; 2. Biotechnology ResearchCenter-Hubei Key Laboratory of Natural Products Use and Research, Three Gorges University, Yichang 443002, Hubei, China

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1. Administration of Shennongjia Nature Reserve, Shennongjia 442421, Hubei, China;

2. Biotechnology ResearchCenter-Hubei Key Laboratory of Natural Products Use and Research, Three Gorges University, Yichang 443002, Hubei, China