Germination of <i>Pinus tabulaeformis</i> seeds with PEG 6000 osmotic treatments

SHI Feng-hou; ZHU Can-can; SHEN Yong-bao; SHI Ji-sen

Volume 25 Issue 3

Jun. 2018

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Article Navigation > Journal of Zhejiang A&F University > 2008 > 25(3): 289-292

SHI Feng-hou, ZHU Can-can, SHEN Yong-bao, et al. Germination of Pinus tabulaeformis seeds with PEG 6000 osmotic treatments[J]. Journal of Zhejiang A&F University, 2008, 25(3): 289-292.

Citation:

SHI Feng-hou, ZHU Can-can, SHEN Yong-bao, et al. Germination of Pinus tabulaeformis seeds with PEG 6000 osmotic treatments[J]. Journal of Zhejiang A&F University, 2008, 25(3): 289-292.

Germination of Pinus tabulaeformis seeds with PEG 6000 osmotic treatments

1.
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received Date: 2007-05-25
Rev Recd Date: 2007-09-02
Publish Date: 2008-06-10

Abstract

The effects of polyethylene glycol(PEG) 6000 osmotic treatments on germination of Pinus tabulaeformis seeds were studied through an orthogonal design(L934, L1645). Seeds harvested in 2002 had differing water content, and seeds harvested in 2004 were artificially aged(40 ℃, 100%RH) to different time periods. These seeds were then subjected to different pretreatment combinations of PEG solution level, treatment duration, and temperature. Next, seeds were germinated on a cotton bed, and the germination rate, germination index(GI=∑Gt(germination rate in certain days) /Dt(number of germination days) ), and vigor index(VI=GI/fresh weight of seedling) were statistically analyzed. Seeds harvested in 2002 had a water content of either 9.84% or 6.03%, were treated with a 200 g·kg-1 PEG solution, and had a treatment time of 1 d; seeds harvested in 2004 were artificially aged 0 d, were treated with a 200 g·kg-1 PEG solution, had a treatment temperature of 25 ℃, and a treatment duration of 2 d. The seed germination rate was higher(P<0.05) after treatment and seeds sprouted synchronously. The range of water content for seed germination was wide with seed germination being inhibited at lower seed water content. Seed vigor decreases due to natural aging that were simulated with artificial aging were restored with PEG treatment of the artificially aged seeds. The different content, treatment duration, and temperature of the PEG solution had a definite influence on the seeds with the PEG treatments, overall, helpful for Chinese pine seed germination.
- silviculture,
- Pinus tabulaeformis,
- PEG 6000,
- germination rate,
- germination index,
- vigor index

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Germination of Pinus tabulaeformis seeds with PEG 6000 osmotic treatments

1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received Date: 2007-05-25

Rev Recd Date: 2007-09-02

Publish Date: 2008-06-10

Key words:

Abstract: The effects of polyethylene glycol(PEG) 6000 osmotic treatments on germination of Pinus tabulaeformis seeds were studied through an orthogonal design(L934, L1645). Seeds harvested in 2002 had differing water content, and seeds harvested in 2004 were artificially aged(40 ℃, 100%RH) to different time periods. These seeds were then subjected to different pretreatment combinations of PEG solution level, treatment duration, and temperature. Next, seeds were germinated on a cotton bed, and the germination rate, germination index(GI=∑Gt(germination rate in certain days) /Dt(number of germination days) ), and vigor index(VI=GI/fresh weight of seedling) were statistically analyzed. Seeds harvested in 2002 had a water content of either 9.84% or 6.03%, were treated with a 200 g·kg-1 PEG solution, and had a treatment time of 1 d; seeds harvested in 2004 were artificially aged 0 d, were treated with a 200 g·kg-1 PEG solution, had a treatment temperature of 25 ℃, and a treatment duration of 2 d. The seed germination rate was higher(P<0.05) after treatment and seeds sprouted synchronously. The range of water content for seed germination was wide with seed germination being inhibited at lower seed water content. Seed vigor decreases due to natural aging that were simulated with artificial aging were restored with PEG treatment of the artificially aged seeds. The different content, treatment duration, and temperature of the PEG solution had a definite influence on the seeds with the PEG treatments, overall, helpful for Chinese pine seed germination.

Germination of Pinus tabulaeformis seeds with PEG 6000 osmotic treatments

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