AFLP-based sexual identification in <EM>Torreya grandis</EM>

LIANG Dan; ZHOU Qin; SHU Yan; ZENG Yan-ru; YU Wei-wu

Volume 26 Issue 1

Feb. 2009

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LIANG Dan, ZHOU Qin, SHU Yan, ZENG Yan-ru, YU Wei-wu. AFLP-based sexual identification in Torreya grandis[J]. Journal of Zhejiang A&F University, 2009, 26(1): 63-67.

Citation:

LIANG Dan, ZHOU Qin, SHU Yan, ZENG Yan-ru, YU Wei-wu. AFLP-based sexual identification in Torreya grandis[J]. Journal of Zhejiang A&F University, 2009, 26(1): 63-67.

AFLP-based sexual identification in Torreya grandis

1.
Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: ZENG Yan-ru
Received Date: 2008-01-31
Rev Recd Date: 2008-10-06
Publish Date: 2009-02-20

Abstract

Torreya trees can be monoecious or dioecious，and if they are monoecious，the male and female cones may be on different branches. To identify young male and female seedlings of T. grandis，an established AFLP（amplified fragment length polymorphism） analysis protocol along with thymine-adenine （T-A） cloning and sequencing were applied to male and female T. grandis seedlings using 15 pairs of primers. Results showed that the E-AGC/M-CAT marker revealed a band restricted to the female whose sequence was not matched to any sequence from GenBank. This female-specific band was only available in the female，monoecious T. grandis trees. It was inferred that monoecious trees were likely derived from female trees. These results have laid a foundation for subsequent studies. ［Ch，6 fig. 12 ref.］
- cash forestry,
- Torreya grandis,
- AFLP（amplified fragment length polymorphism）,
- leaf,
- T-A cloning and sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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AFLP-based sexual identification in Torreya grandis

1. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

Corresponding author: ZENG Yan-ru

Received Date: 2008-01-31

Rev Recd Date: 2008-10-06

Publish Date: 2009-02-20

Key words:

cash forestry /
Torreya grandis /
AFLP（amplified fragment length polymorphism） /
leaf /
T-A cloning and sequencing

Abstract: Torreya trees can be monoecious or dioecious，and if they are monoecious，the male and female cones may be on different branches. To identify young male and female seedlings of T. grandis，an established AFLP（amplified fragment length polymorphism） analysis protocol along with thymine-adenine （T-A） cloning and sequencing were applied to male and female T. grandis seedlings using 15 pairs of primers. Results showed that the E-AGC/M-CAT marker revealed a band restricted to the female whose sequence was not matched to any sequence from GenBank. This female-specific band was only available in the female，monoecious T. grandis trees. It was inferred that monoecious trees were likely derived from female trees. These results have laid a foundation for subsequent studies. ［Ch，6 fig. 12 ref.］

AFLP-based sexual identification in Torreya grandis

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通讯作者: 陈斌, bchen63@163.com

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AFLP-based sexual identification in Torreya grandis

1. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

Corresponding author: ZENG Yan-ru

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通讯作者: 陈斌, bchen63@163.com

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AFLP-based sexual identification in Torreya grandis

1. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

Corresponding author: ZENG Yan-ru

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