Volume 26 Issue 4
Aug.  2009
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Article Navigation >  Journal of Zhejiang A&F University  > 2009  >  26(4): 467-472

JIN Wanzhou, BU Jing, LUO Huiwen, et al. Floristic characteristics of seed plants in Qinghai area of Qilian Mountain National Park[J]. Journal of Zhejiang A&F University, 2022, 39(2): 289-296. DOI: 10.11833/j.issn.2095-0756.20210343
Citation: ZHENG Bing-song, CHEN Miao, CHU Huai-liang, et al. Auxin response factor fragments cloned by cDNA-AFLP during Carya cathayensis grafting process[J]. Journal of Zhejiang A&F University, 2009, 26(4): 467-472.
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Auxin response factor fragments cloned by cDNA-AFLP during Carya cathayensis grafting process

  • 1.

    The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

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  • Corresponding author: ZHENG Bing-song
  • Publish Date: 2009-08-20
  • In order to improve survival of Carya cathayensis（Chinese hickory） grafted seedling，a cDNA fragment related to Chinese hickory graft was obtained by cDNA-amplified fragment length polymorphism （cDNA-AFLP）techniques during the grafting process. Analyses were conducted using the National Center for Biotechnology Information（NCBI）homology comparison and Fluorescence Real Time Polymerase Chain Reaction（RT-PCR）. The NCBI homology comparison showed that a cDNA fragment，named CcARF，was 81% homologous with a wheat section of the auxin response factor（ARF）. Meanwhile，RT-PCR revealed that before grafting，gene expressions were strongly induced both in the rootstock and scion of Chinese hickory and declined sharply at 3 d after grafting. After 3 d，expression in rootstock was weakly induced. However，in the scion，7 d after grafting，expression increased reaching a maximum 14 d after grafting；this was 4.7 times stronger than 3 d after grafting. Thus，when grafting Chinese hickory，the CcARF fragment may be involved in the regulation of gene expression.[Ch，3 fig. 1 tab. 21 ref.]
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Auxin response factor fragments cloned by cDNA-AFLP during Carya cathayensis grafting process

  • 1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China
    Corresponding author: ZHENG Bing-song
  • Publish Date: 2009-08-20

Abstract: In order to improve survival of Carya cathayensis（Chinese hickory） grafted seedling，a cDNA fragment related to Chinese hickory graft was obtained by cDNA-amplified fragment length polymorphism （cDNA-AFLP）techniques during the grafting process. Analyses were conducted using the National Center for Biotechnology Information（NCBI）homology comparison and Fluorescence Real Time Polymerase Chain Reaction（RT-PCR）. The NCBI homology comparison showed that a cDNA fragment，named CcARF，was 81% homologous with a wheat section of the auxin response factor（ARF）. Meanwhile，RT-PCR revealed that before grafting，gene expressions were strongly induced both in the rootstock and scion of Chinese hickory and declined sharply at 3 d after grafting. After 3 d，expression in rootstock was weakly induced. However，in the scion，7 d after grafting，expression increased reaching a maximum 14 d after grafting；this was 4.7 times stronger than 3 d after grafting. Thus，when grafting Chinese hickory，the CcARF fragment may be involved in the regulation of gene expression.[Ch，3 fig. 1 tab. 21 ref.]

JIN Wanzhou, BU Jing, LUO Huiwen, et al. Floristic characteristics of seed plants in Qinghai area of Qilian Mountain National Park[J]. Journal of Zhejiang A&F University, 2022, 39(2): 289-296. DOI: 10.11833/j.issn.2095-0756.20210343
Citation: ZHENG Bing-song, CHEN Miao, CHU Huai-liang, et al. Auxin response factor fragments cloned by cDNA-AFLP during Carya cathayensis grafting process[J]. Journal of Zhejiang A&F University, 2009, 26(4): 467-472.
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