Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

CHEN Guo-liang; JIANG Jing; QIAO Gui-rong; JIN Liang; SUN Yue-hua; YANG Ye; ZHOU Jing; ZHUO Ren-ying

Volume 26 Issue 5

Oct. 2009

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Article Navigation > Journal of Zhejiang A&F University > 2009 > 26(5): 644-651

CHEN Guo-liang, JIANG Jing, QIAO Gui-rong, JIN Liang, SUN Yue-hua, YANG Ye, ZHOU Jing, ZHUO Ren-ying. Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis[J]. Journal of Zhejiang A&F University, 2009, 26(5): 644-651.

Citation:

CHEN Guo-liang, JIANG Jing, QIAO Gui-rong, JIN Liang, SUN Yue-hua, YANG Ye, ZHOU Jing, ZHUO Ren-ying. Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis[J]. Journal of Zhejiang A&F University, 2009, 26(5): 644-651.

Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

1.
Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;
2.
College of Pastoral Agriculture Science and Technology，Lanzhou University，Lanzhou 730020，Gansu，China

More Information

Corresponding author: ZHUO Ren-ying
Publish Date: 2009-10-20

Abstract

Salt stress is one of the major abiotic stressed over the world. Most of the researchs were foucsed on improve the ability of plant species living in salt soil. Two-dimesional gel electrophoresis（2-DE）is one of the most useful methods in proteins research. To determine total protein extraction efficiency from Populus cathayana with two-dimensional electrophoresis （2-DE） analysis，three protein extraction methods （phenol extraction，improved acetone precipitation method，and TCA-acetone precipitation method） were compared. Electrophoresis conditions and leafy proteomics of P. cathayana with salt stress were also analyzed. Results showed that the three protein extraction methods generated 437，603，and 721 protein spots with the phenol extraction method more effectively removing salt from the protein sample，generating more protein spots，and giving clearer background for the 2-DE map. The results showed that condition of 300 μg per IPG gel，20 min equilibrium time，90 000 V·h IEF time was the suitable treatment. Also，the leafy proteomics analysis revealed that 10 protein spots was down-regulators，32 protein spots was up?-regulators，and 4 new protein spots was induced by salt stress. These proteins may be important response to salt stress. ［Ch，9 fig. 24 ref.］
- botany,
- NaCl stress,
- proteomics,
- two-dimensional electrophoresis （2-DE）,
- Populus cathayana

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

1. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. College of Pastoral Agriculture Science and Technology，Lanzhou University，Lanzhou 730020，Gansu，China

Corresponding author: ZHUO Ren-ying

Publish Date: 2009-10-20

Key words:

botany /
NaCl stress /
proteomics /
two-dimensional electrophoresis （2-DE） /
Populus cathayana

Abstract: Salt stress is one of the major abiotic stressed over the world. Most of the researchs were foucsed on improve the ability of plant species living in salt soil. Two-dimesional gel electrophoresis（2-DE）is one of the most useful methods in proteins research. To determine total protein extraction efficiency from Populus cathayana with two-dimensional electrophoresis （2-DE） analysis，three protein extraction methods （phenol extraction，improved acetone precipitation method，and TCA-acetone precipitation method） were compared. Electrophoresis conditions and leafy proteomics of P. cathayana with salt stress were also analyzed. Results showed that the three protein extraction methods generated 437，603，and 721 protein spots with the phenol extraction method more effectively removing salt from the protein sample，generating more protein spots，and giving clearer background for the 2-DE map. The results showed that condition of 300 μg per IPG gel，20 min equilibrium time，90 000 V·h IEF time was the suitable treatment. Also，the leafy proteomics analysis revealed that 10 protein spots was down-regulators，32 protein spots was up?-regulators，and 4 new protein spots was induced by salt stress. These proteins may be important response to salt stress. ［Ch，9 fig. 24 ref.］

Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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