Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

CHEN Guo-liang; JIANG Jing; QIAO Gui-rong; JIN Liang; SUN Yue-hua; YANG Ye; ZHOU Jing; ZHUO Ren-ying

Volume 26 Issue 5

Oct. 2009

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2009 > 26(5): 644-651

CHEN Guo-liang, JIANG Jing, QIAO Gui-rong, JIN Liang, SUN Yue-hua, YANG Ye, ZHOU Jing, ZHUO Ren-ying. Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis[J]. Journal of Zhejiang A&F University, 2009, 26(5): 644-651.

Citation:

CHEN Guo-liang, JIANG Jing, QIAO Gui-rong, JIN Liang, SUN Yue-hua, YANG Ye, ZHOU Jing, ZHUO Ren-ying. Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis[J]. Journal of Zhejiang A&F University, 2009, 26(5): 644-651.

Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

1.
Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;
2.
College of Pastoral Agriculture Science and Technology，Lanzhou University，Lanzhou 730020，Gansu，China

More Information

Corresponding author: ZHUO Ren-ying
Publish Date: 2009-10-20

Abstract

Salt stress is one of the major abiotic stressed over the world. Most of the researchs were foucsed on improve the ability of plant species living in salt soil. Two-dimesional gel electrophoresis（2-DE）is one of the most useful methods in proteins research. To determine total protein extraction efficiency from Populus cathayana with two-dimensional electrophoresis （2-DE） analysis，three protein extraction methods （phenol extraction，improved acetone precipitation method，and TCA-acetone precipitation method） were compared. Electrophoresis conditions and leafy proteomics of P. cathayana with salt stress were also analyzed. Results showed that the three protein extraction methods generated 437，603，and 721 protein spots with the phenol extraction method more effectively removing salt from the protein sample，generating more protein spots，and giving clearer background for the 2-DE map. The results showed that condition of 300 μg per IPG gel，20 min equilibrium time，90 000 V·h IEF time was the suitable treatment. Also，the leafy proteomics analysis revealed that 10 protein spots was down-regulators，32 protein spots was up?-regulators，and 4 new protein spots was induced by salt stress. These proteins may be important response to salt stress. ［Ch，9 fig. 24 ref.］
- botany,
- NaCl stress,
- proteomics,
- two-dimensional electrophoresis （2-DE）,
- Populus cathayana

Relative Article

[1]	SHEN Xuyue, ZHANG Lang, CHEN Rongrong, SHEN Yamei, JIN Hexian. Effect of NaCl stress on the morphology and related physiological indexes of Magnolia biondii seedlings . Journal of Zhejiang A&F University, 2021, 38(2): 289-295. doi: 10.11833/j.issn.2095-0756.20200449
[2]	LI Yehua, CHEN Shuang, ZHAO Bing. Effect of aluminum stress on growth and physiological characteristics of Hydrangea tissue culture seedlings . Journal of Zhejiang A&F University, 2020, 37(6): 1064-1070. doi: 10.11833/j.issn.2095-0756.20200121
[3]	GAO Fan, XIE Yue, SHEN Yanqiu, LEI Zhi, WANG Xiu, XIA Hui, LIANG Dong. Exogenous melatonin for NaCl stress with antioxidant enzymes and osmotic substances of Aclinidia deliciosa seedlings . Journal of Zhejiang A&F University, 2018, 35(2): 291-297. doi: 10.11833/j.issn.2095-0756.2018.02.013
[4]	WU Jun, LI Yingang, LUO Xiubao, LIU Xinhong, SHI Congguang, HE Yunhe. Seed biological characteristics of Styrax tonkinensis . Journal of Zhejiang A&F University, 2014, 31(1): 9-13. doi: 10.11833/j.issn.2095-0756.2014.01.002
[5]	HE Qijiang, LI Nan, FU Maoyi, ZHOU Wenwei, WANG Bo. Root activity and cell membrane permeability in Phyllostachys violascens with NaCl stress . Journal of Zhejiang A&F University, 2013, 30(6): 944-949. doi: 10.11833/j.issn.2095-0756.2013.06.021
[6]	SHEN Ya-mei, QIAN Chao, FAN Yi-rong, TONG Zai-kang. Pollen morphology of 9 Magnolia species and 3 cultivars . Journal of Zhejiang A&F University, 2012, 29(3): 394-400. doi: 10.11833/j.issn.2095-0756.2012.03.011
[7]	HE Yong-qing, FANG Jia, YU Min-fen, FANG Zhong-xiang, JIANG Bo, PAN Yin-hui, ZHENG Bing-song. Advances in molecular biology for plasma membrane intrinsic proteins （PIPs）：a review . Journal of Zhejiang A&F University, 2012, 29(3): 446-452. doi: 10.11833/j.issn.2095-0756.2012.03.020
[8]	MA Jin, LIU Zhi-gao, ZHENG Gang. Differential proteomics and its application in plant salt stress . Journal of Zhejiang A&F University, 2011, 28(1): 139-143. doi: 10.11833/j.issn.2095-0756.2011.01.022
[9]	ZHANG Xiao-guo, QIAO Gui-rong, LI Cui-yun, LIU Ming-ying, JIANG Jing, LI Hai-ying, QIU Wen-min, ZHUO Ren-ying. A two-dimensional electrophoresis system of Salix matsudana root proteomics . Journal of Zhejiang A&F University, 2011, 28(4): 653-661. doi: 10.11833/j.issn.2095-0756.2011.04.021
[10]	KONG Hong, CHENG Fang-yun. Cytological and molecular biological evidence for taxonomic revision of Paeonia delavayi . Journal of Zhejiang A&F University, 2010, 27(4): 601-605. doi: 10.11833/j.issn.2095-0756.2010.04.021
[11]	SUI De-zong, WANG Bao-song, SHI Shi-zheng, JIAO Zhong-yi. Growth and photosynthesis of shrub willow clones with salt stress . Journal of Zhejiang A&F University, 2010, 27(1): 63-68. doi: 10.11833/j.issn.2095-0756.2010.01.010
[12]	ZHU Xiao-lou, LOU Lu-huan, WANG Hui. Physiological and biochemical characteristics of four species of Carex with drought stress . Journal of Zhejiang A&F University, 2009, 26(5): 656-662.
[13]	YANG Ye, JIANG Jing, QIAO Gui-rong, ZHOU Jing, CHEN Yin, HE Zheng-quan, LI Hai-ying, ZHUO Ren-ying. Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1 . Journal of Zhejiang A&F University, 2009, 26(4): 473-478.
[14]	HU Bin, FAN Jun-feng, GAO Jian-she, ZHOU Yong-xue. One-year-old seedling traits of Populus deltoides hybrids with P. cathayana，P. szechuanica，and P. purdomii . Journal of Zhejiang A&F University, 2009, 26(6): 778-783.
[15]	MA Hai-yan, LIN Song-ming, XU Ying-chun, LI Yong-rong, CHEN Yong-hui. Salt(NaCl) stress on growth,ion uptake,and transport of (Taxodium distichum×T. mucronatum)×T.mucronatum ‘Zhongshansha’ breeding clones . Journal of Zhejiang A&F University, 2008, 25(3): 319-323.
[16]	XIANG Zuo-xiang, XUGui-fang, JIANG Wen-jun. Drought stress on physiological and biochemical processes in four spiny plant species . Journal of Zhejiang A&F University, 2007, 24(1): 7-11.
[17]	HUANG You-jun, WANG Zheng-jia, ZHENG Bing-song, HUANG Xiao-min, HUANG Jian-qin. Anatomy of stamen development on Carya cathayensis . Journal of Zhejiang A&F University, 2006, 23(1): 56-60.
[18]	RUAN Xiu-chun, SI Jin-ping, WU Jian, HUANG Wen-hua, GUO Bao-lin, XU Yuan-ke. Preliminary study on biological characteristics and ecological adaptability of Tripterygium . Journal of Zhejiang A&F University, 2006, 23(5): 595-598.
[19]	LIN Xin-chun, YU Zhi-xiong. Characters of leaf epidermis of Magnoliaceae and its taxonomic significance . Journal of Zhejiang A&F University, 2004, 21(1): 33-39.
[20]	GAO Jian-she, LIU Yu-yuan, FU Yu-qin, FU Jun, WANG Jun, YANG Zi-xiang. On growth character of young trees of Populus cathayana of different provenances . Journal of Zhejiang A&F University, 2004, 21(1): 115-118.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3551) PDF downloads(138) Cited by()

Proportional views

Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

1. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

2. College of Pastoral Agriculture Science and Technology，Lanzhou University，Lanzhou 730020，Gansu，China

Corresponding author: ZHUO Ren-ying

Publish Date: 2009-10-20

Key words:

botany /
NaCl stress /
proteomics /
two-dimensional electrophoresis （2-DE） /
Populus cathayana

Abstract: Salt stress is one of the major abiotic stressed over the world. Most of the researchs were foucsed on improve the ability of plant species living in salt soil. Two-dimesional gel electrophoresis（2-DE）is one of the most useful methods in proteins research. To determine total protein extraction efficiency from Populus cathayana with two-dimensional electrophoresis （2-DE） analysis，three protein extraction methods （phenol extraction，improved acetone precipitation method，and TCA-acetone precipitation method） were compared. Electrophoresis conditions and leafy proteomics of P. cathayana with salt stress were also analyzed. Results showed that the three protein extraction methods generated 437，603，and 721 protein spots with the phenol extraction method more effectively removing salt from the protein sample，generating more protein spots，and giving clearer background for the 2-DE map. The results showed that condition of 300 μg per IPG gel，20 min equilibrium time，90 000 V·h IEF time was the suitable treatment. Also，the leafy proteomics analysis revealed that 10 protein spots was down-regulators，32 protein spots was up?-regulators，and 4 new protein spots was induced by salt stress. These proteins may be important response to salt stress. ［Ch，9 fig. 24 ref.］

Populus leafy proteomics with NaCl stress using two-dimensional electrophoresis analysis

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views