An antioxidant system in cucumber seedling leaves with short term drought stress
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摘要: 以黄瓜津优1号Cucumis sativusJinyou No. 1为试材,采用水培法,通过在营养液中添加不同比例聚乙二醇PEG 6000模拟干旱胁迫,研究短期干旱胁迫对黄瓜幼苗叶片抗氧化系统的影响。结果显示:在不同程度干旱胁迫下处理24 h后,超氧化物歧化酶(SOD),谷胱甘肽还原酶(GR),脱氢抗坏血酸还原酶(DHAR)和单脱氢抗坏血酸还原酶(MDHAR)活性以及抗坏血酸(ASA)和还原型谷胱甘肽(GSH)质量摩尔浓度均下降,其中超氧化物歧化酶和谷胱甘肽还原酶活性、抗坏血酸和还原性谷胱甘肽GSH质量摩尔浓度与对照比较有显著差异(P<0.05);而过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性、可溶性蛋白质量分数和丙二醛(MDA)质量摩尔浓度在胁迫条件下升高,其中可溶性蛋白和丙二醛质量摩尔浓度增加明显(P<0.05);过氧化物酶(POD)活性则呈现出中度胁迫下显著升高(P<0.05)和重度胁迫下略有下降的趋势。以上表明:短期干旱胁迫引起了黄瓜叶片膜脂过氧化加剧,酶促抗氧化系统比非酶促系统在抗氧化过程中起到了更积极的作用。图3参21Abstract: To determine the effect of short term drought stress on an antioxidant system in cucumber seedling leaves, the Cucumis sativus Jinyou No. 1 seedlings were cultured in a shanqi nutrient solution under drought stress simulated by 5% and 10% PEG 6000. A control, 5% and 10% PEG 6000 with three replications was used to compare superoxide dismutase (SOD), glutathione reductase (GR), ascorbate (ASA), glutathione (GSH), soluble protein, malondialdehyde (MDA). Results after 24 h of treatment showed that compared to the control, SOD and GR activities as well as ASA and GSH content significantly decreased (P<0.05). However, soluble protein and MDA content significantly increased (P<0.05). Thus, short term stress resulted in acceleration of the membrane lipid peroxidation, and the enzymatic antioxidant system played a more active role in the process of antioxidant. [Ch, 3 fig. 21 ref.]
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Key words:
- horticulture /
- cucumber /
- drought stress /
- antioxidant system
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2015.02.017

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