Biocontrol agents introduced into a Cucumis melo rhizosphere
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摘要: 通过对土壤中可培养微生物的种群计数和土壤酶质量分数变化的测定，研究了引入黄瓜Cucumis melo根围土壤2株生防菌株Brevibacillus brevis ZJY-1和Bacillus subtilis ZJY-116的环境生态效应。结果表明，生防菌株的施入对土壤中细菌的种群数量有短期影响，但随着黄瓜生育期的延长，施入生防细菌土壤的细菌数最终与对照土壤中细菌数持平；生防细菌的施入对土壤中真菌和放线菌的种群数量影响不大，在取样各时期，经外源菌株处理的土壤中真菌和放线菌数量与对照相比差异均不显著。土壤酶质量分数的测定结果表明，除过氧化氢酶外，在植株生育期，2株生防细菌的引入对土壤中蔗糖酶、脲酶和脱氢酶的质量分数均有不同程度的影响。酶活性普遍高于对照，可见，外源细菌的引入可提高土壤的肥力水平。Abstract: By quantifying cultural microorganisms and analyzing activities of enzymes in the soil, the ecological effect of biocontrol agents, Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116, introduced into the Cucumis melo (cucumber) rhizosphere was studied to improve soil fertility. We used potculture method with three replications, one replication having ten pots of cucumber seedlings with the agent treated soil. 10 g soil was taken to measure every other week after the seedlings come out. Results showed that the introduced strains initially increased the bacterial population, but the influence gradually diminished with further plant growing. Introduction of the two strains caused no significant differences in fungi (P>0.01). Compared to the untreated soil, enzyme activities showed that during the plant growing season, the two biocontrol agents increased fructosidase, and dehydrogenase activity. Thus, the study indicated that introduction of the two strains could increase soil fertility.
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Key words:
- pedology /
- biocontrol agents /
- Cucumis melo (cucumber) /
- rhizosphere /
- ecological effects
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