Pollen-wall protein extraction and characteristics of Catalpa bungei
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摘要: 为了进一步研究楸树Catalpa bungei自交不亲和特性,以楸树不同产地植株云台山楸树(CB-1),老山楸树(CB-2)和滇楸Catalpa fargesi f. duclouxii (CF)的花粉为对象,利用普通研磨法及超声波破碎法,研究了适合于花粉壁蛋白质提取的方法。同时采用考马斯亮蓝染色法及聚丙烯酰胺凝胶电泳(SDS?鄄PAGE)比较了不同树种花粉蛋白质质量分数及其主要蛋白质组分。结果表明:超声波破碎法适于花粉壁蛋白质的提取,最佳超声参数为功率400 W,超声时间 3 s次-1,间歇时间 6 s次-1,3个回合,工作120次,相隔5 min回合-1。蛋白质提取液三羟甲基氨基甲烷(Tris-HCl)的pH 7.8最佳。不同树种的花粉壁蛋白质质量分数表现为滇楸最高,云台山楸树和老山楸树较低。蛋白质组分研究表明:各树种花粉共有蛋白质有26个,特异蛋白质为连楸53.8 kD,35.0 kD,23.4 kD,26.5 kD;老楸53.8 kD,38.5 kD,23.4 kD;滇楸38.5 kD,26.5 kD。图6表1参18
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关键词:
- 植物学 /
- 楸树 /
- 超声波破碎法 /
- 蛋白质 /
- 聚丙烯酰胺凝胶电泳(SDS-PAGE)
Abstract: The research was conducted to further reveal the self-incompatibility that exists extensively Catalpa bungei and to evaluate the best way to extract pollen-wall protein of Catalpa, including the Catalpa bungei plants CB-1 from Yuntaishan National Forest Park of Lianyungang and CB-2 from Laoshan Forest of Nanjing and Catalpa fargesii f. duclouxii (CF) using incorporation and ultrasonication. Also, the content and component of pollen-wall protein from different varieties was compared by the Coomassie Blue Staining Method and SDS (sodium dodecyl sulfate)-polyacrylamide gel electrophoresis (PAGE). Results showed that the relative optimization method to extract pollen-wall protein of C. bungei was ultrasonication with the optimal parameters being: ultrasonication at 400 W power for 3 s every 6 s for 3 rounds taking 120 duplications at an interval of 5 min. The optimal pH value to extract pollen-wall protein was pH 7.8(F>F0.05). For pollen-wall protein content, CF was higher(F>F0.01) than both CB-1 and CB-2. From the pollen of these three catalpas, 26 proteins were found. Molecular weight of the specific proteins for CB-1 were 53.8 kDa, 35.0 kDa, 23.4 kDa, and 26.5 kDa; for CB-2 they were 53.8 kDa, 38.5 kDa, and 23.4 kDa; and for CF they were 8.5 kDa and 26.5 kDa.[Ch, 6 fig. 1 tab. 18 ref.]-
Key words:
- botany /
- Catalpa bungei /
- ultrasonication /
- protein /
- SDS-PAGE
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2016.01.009

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