Reflection spectrum and photochemical characteristics of different colors’ leaves in Pseudosasa japonica
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摘要:
目的 通过解析矢竹Pseudosasa japonica不同叶色叶片反射光谱特性、光系统Ⅱ(PSⅡ)和光系统Ⅰ(PSⅠ)特性之间的差异,探索不同叶色竹种光合能力差异,从生理角度分析矢竹叶色变异特性并为进一步探究叶色变异机制奠定基础。 方法 取生长健壮的矢竹叶片(GL)、花叶矢竹P. japonica f. akebonosuji复绿叶片(AL)、花叶矢竹条纹叶片[(SL)包括白色部分(SA)和绿色部分(SG)]、曙筋矢竹P. japonica f. akebono(VL)4种不同叶色叶片为材料,测定其光合色素质量分数、叶绿素归一化指数(ChlNDI)、光化学植被指数(PRI)、快速荧光动力学参数及820 nm相对吸收值。 结果 叶片叶绿素质量分数从大到小依次为GL、SL、VL、AL;不同叶色矢竹叶片的叶绿素归一化指数、光化学植被指数变化趋势一致,从大到小依次均为GL、SG、VL、SA、AL;4个色叶的光系统Ⅰ最大氧化还原能力从大到小依次为GL、VL、SG、AL;花叶矢竹复绿叶、条纹绿叶和曙筋矢竹都具有叶绿素荧光曲线动力学活性,但光系统Ⅱ反应中心开放降低程度与矢竹差异显著(P<0.05),能量用于电子传递份额变小;缺乏叶绿素使得单位反应中心吸收的光能不断增加,可能是因为它需要更多的反应中心来应对其较低的转化效率,但最大光化学效率(Fv/Fm)和叶片性能指数(PIABS)都逐渐降低,可能是光系统Ⅱ反应中心发生可逆失活,能吸收光能但不能推动电子传递。 结论 叶色变异导致矢竹叶片光合色素质量分数存在差异,进而影响叶绿素归一化指数和光化学反射指数特征参数,叶绿素缺乏会影响光系统Ⅱ活性反应中心发生可逆性失活。花叶矢竹条纹叶片反应中心较少,但仍具有较好的光系统Ⅱ活性和叶绿素水平,维持较好的光合能力,这可能与其独特的花叶性状有关。图8表2参31 Abstract:Objective This study is aimed to explore the differences in photosynthetic capacity of different colors’leaves of Pseudosasa japonica, analyze the leaf color variation from a physiological point of view and lay the foundation for the further exploration of the mechanism of leaf color variation. Method With the strong bamboo leaves of Pseudosasa japonica(GL), regreened leaves of P. japonica f. akebonosuji(AL), striped leaves of P. japonica f. akebonosuj(SL), including the white part(SA) and the green part(SG), and the leaves of P. japonica f. akebono(VL) selected as the subjects, an investigation was conducted of the photosynthetic pigment content, ChlNDI, PRI, fast fluorescence kinetic parameters and 820 nm relative absorption. Result a) The relative content of chlorophyll in leaves was as follows: GL>SL>VL>AL and the change trend of chlorophyll normalized difference index (ChlNDI) and photochemical reflectance index(PRI) in different leaves of Pseudosasa japonica is the same, which is GL>SG>VL>SA>AL; b) The maximum redox capacity of Photosystem Ⅰ(PSⅠ) of three bamboo species was GL>VL>SG>AL; the regreened leaves and the striped green leaves of P. japonica f. akebonosuji and the P. japonica f. akebono demonstrate chlorophyll fluorescence curve kinetic activity, but the Photosystem Ⅱ(PSⅡ) reaction center had a significantly lower degree of openness than that of the Pseudosasa japonica, and the share of energy used for electron transfer becomes smaller; c) The lack of chlorophyll makes the light energy absorbed by the unit reaction centers increase continuously, probably because it requires more reaction centers to cope with its lower conversion efficiency, however, the maximum photochemical efficiency (Fv/Fm) and the leaf performance index on absorption basis (PIABS) are gradually reduced, possibly due to the fact that the PSII reaction center is reversibly deactivated, able to absorb light energy yet unable to promote electron transfer. Conclusion The variation of leaf color will lead to the difference of photosynthetic pigment content in different kinds of Pseudosasa japonica, and then affect the chlorophyll normalization index and photochemical reflectance index characteristic parameters. Chlorophyll deficiency will affect the active reaction center of PSⅡ, causing reversible inactivation. There are fewer reaction centers in the striped leaves of P. japonica f. akebonosuji, but it still demonstrates good PSⅡ activity, chlorophyll level, and maintains good photosynthetic capacity, usually subject to the uniqueness of flowers and features of leaves. [Ch, 8 fig. 2 tab. 31 ref.] -
Key words:
- Pseudosasa japonica /
- leaf color variation /
- reflectance spectrum /
- photoreaction system
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表 1 不同叶色矢竹类光合色素差异
Table 1. Difference of photosynthetic pigment content in different colors’ leaves of P. japonica
材料 Chla/(mg·g−1) Chlb/(mg·g−1) Car/(mg·g−1) Chl a+b/(mg·g−1) Chl a/b Car/Chl AL 0.34±0.015 a 0.26±0.010 a 0.32±0.015 c 0.60±0.035 a 1.3±0.007 a 0.53±0.022 b SG 20.39±0.170 b 18.27±0.120 b 5.52±0.020 a 38.66±0.250 c 1.1±0.020 b 0.14±0.010 a VL 17.09±0.080 b 15.72±0.070 b 4.07±0.184 b 33.81±0.140 b 1.0±0.013 b 0.12±0.007 a GL 22.19±0.120 c 19.66±0.150 b 4.89±0.020 a 41.85±0.220 c 1.14±0.024 b 0.11±0.005 a 说明:同列内不同小写字母表示材料间差异显著(P<0.05) 表 2 叶片ChlNDI和PRI与色素质量分数、Fv/Fm、PIABS、φE0的相关性
Table 2. Correlation between reflectance spectrum parameters and pigment contents
光谱参数 Chla Chlb Chl a+b Car Fv/Fm PIABS φEo ChlNDI 0.966** 0.961** 0.924** 0.898* 0.883* 0.802* 0.937** PRI 0.969** 0.977** 0.931** 0.929** 0.759* 0.648 0.823* 说明:*表示P<0.05,**表示P<0.01 -
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