Reflection spectrum and photochemical characteristics of different colors’ leaves in Pseudosasa japonica
-
摘要:
目的 通过解析矢竹Pseudosasa japonica不同叶色叶片反射光谱特性、光系统Ⅱ(PSⅡ)和光系统Ⅰ(PSⅠ)特性之间的差异，探索不同叶色竹种光合能力差异，从生理角度分析矢竹叶色变异特性并为进一步探究叶色变异机制奠定基础。 方法 取生长健壮的矢竹叶片(GL)、花叶矢竹P. japonica f. akebonosuji复绿叶片(AL)、花叶矢竹条纹叶片[(SL)包括白色部分(SA)和绿色部分(SG)]、曙筋矢竹P. japonica f. akebono(VL)4种不同叶色叶片为材料，测定其光合色素质量分数、叶绿素归一化指数(ChlNDI)、光化学植被指数(PRI)、快速荧光动力学参数及820 nm相对吸收值。 结果 叶片叶绿素质量分数从大到小依次为GL、SL、VL、AL；不同叶色矢竹叶片的叶绿素归一化指数、光化学植被指数变化趋势一致，从大到小依次均为GL、SG、VL、SA、AL；4个色叶的光系统Ⅰ最大氧化还原能力从大到小依次为GL、VL、SG、AL；花叶矢竹复绿叶、条纹绿叶和曙筋矢竹都具有叶绿素荧光曲线动力学活性，但光系统Ⅱ反应中心开放降低程度与矢竹差异显著(P＜0.05)，能量用于电子传递份额变小；缺乏叶绿素使得单位反应中心吸收的光能不断增加，可能是因为它需要更多的反应中心来应对其较低的转化效率，但最大光化学效率(Fv/Fm)和叶片性能指数(PIABS)都逐渐降低，可能是光系统Ⅱ反应中心发生可逆失活，能吸收光能但不能推动电子传递。 结论 叶色变异导致矢竹叶片光合色素质量分数存在差异，进而影响叶绿素归一化指数和光化学反射指数特征参数，叶绿素缺乏会影响光系统Ⅱ活性反应中心发生可逆性失活。花叶矢竹条纹叶片反应中心较少，但仍具有较好的光系统Ⅱ活性和叶绿素水平，维持较好的光合能力，这可能与其独特的花叶性状有关。图8表2参31 Abstract:Objective This study is aimed to explore the differences in photosynthetic capacity of different colors’leaves of Pseudosasa japonica, analyze the leaf color variation from a physiological point of view and lay the foundation for the further exploration of the mechanism of leaf color variation. Method With the strong bamboo leaves of Pseudosasa japonica(GL), regreened leaves of P. japonica f. akebonosuji(AL), striped leaves of P. japonica f. akebonosuj(SL), including the white part(SA) and the green part(SG), and the leaves of P. japonica f. akebono(VL) selected as the subjects, an investigation was conducted of the photosynthetic pigment content, ChlNDI, PRI, fast fluorescence kinetic parameters and 820 nm relative absorption. Result a) The relative content of chlorophyll in leaves was as follows: GL＞SL＞VL＞AL and the change trend of chlorophyll normalized difference index (ChlNDI) and photochemical reflectance index(PRI) in different leaves of Pseudosasa japonica is the same, which is GL＞SG＞VL＞SA＞AL; b) The maximum redox capacity of Photosystem Ⅰ(PSⅠ) of three bamboo species was GL＞VL＞SG＞AL; the regreened leaves and the striped green leaves of P. japonica f. akebonosuji and the P. japonica f. akebono demonstrate chlorophyll fluorescence curve kinetic activity, but the Photosystem Ⅱ(PSⅡ) reaction center had a significantly lower degree of openness than that of the Pseudosasa japonica, and the share of energy used for electron transfer becomes smaller; c) The lack of chlorophyll makes the light energy absorbed by the unit reaction centers increase continuously, probably because it requires more reaction centers to cope with its lower conversion efficiency, however, the maximum photochemical efficiency (Fv/Fm) and the leaf performance index on absorption basis (PIABS) are gradually reduced, possibly due to the fact that the PSII reaction center is reversibly deactivated, able to absorb light energy yet unable to promote electron transfer. Conclusion The variation of leaf color will lead to the difference of photosynthetic pigment content in different kinds of Pseudosasa japonica, and then affect the chlorophyll normalization index and photochemical reflectance index characteristic parameters. Chlorophyll deficiency will affect the active reaction center of PSⅡ, causing reversible inactivation. There are fewer reaction centers in the striped leaves of P. japonica f. akebonosuji, but it still demonstrates good PSⅡ activity, chlorophyll level, and maintains good photosynthetic capacity, usually subject to the uniqueness of flowers and features of leaves. [Ch, 8 fig. 2 tab. 31 ref.] -
Key words:
- Pseudosasa japonica /
- leaf color variation /
- reflectance spectrum /
- photoreaction system
-
图 2 叶片反射光谱曲线(A)和一阶导数曲线(B)
Figure 2 Leaf reflectance curve (A) and first derivative curve (B)
图 5 不同叶色叶片的OJIP荧光曲线(A)与相对可变荧光强度随时间的变化(B)
Figure 5 Changes of OJIP fluorescence curves (A) and relative variable fluorescence intensity (B) of different colors’ leaves
图 6 不同叶色叶片820 nm相对吸收值变化
Figure 6 Changes in relative absorption values of PSI 820 nm of different color leaves
图 7 不同叶色叶片PSⅠ最大氧化还原能力ΔI/I0的差异
Figure 7 PSI maximum ability of oxido-reduction △I/I0 of different color leaves
表 1 不同叶色矢竹类光合色素差异
Table 1. Difference of photosynthetic pigment content in different colors’ leaves of P. japonica
材料 Chla/(mg·g−1) Chlb/(mg·g−1) Car/(mg·g−1) Chl a+b/(mg·g−1) Chl a/b Car/Chl AL 0.34±0.015 a 0.26±0.010 a 0.32±0.015 c 0.60±0.035 a 1.3±0.007 a 0.53±0.022 b SG 20.39±0.170 b 18.27±0.120 b 5.52±0.020 a 38.66±0.250 c 1.1±0.020 b 0.14±0.010 a VL 17.09±0.080 b 15.72±0.070 b 4.07±0.184 b 33.81±0.140 b 1.0±0.013 b 0.12±0.007 a GL 22.19±0.120 c 19.66±0.150 b 4.89±0.020 a 41.85±0.220 c 1.14±0.024 b 0.11±0.005 a 说明：同列内不同小写字母表示材料间差异显著(P＜0.05) 
下载: 导出CSV
表 2 叶片ChlNDI和PRI与色素质量分数、Fv/Fm、PIABS、φE0的相关性
Table 2. Correlation between reflectance spectrum parameters and pigment contents
光谱参数 Chla Chlb Chl a+b Car Fv/Fm PIABS φEo ChlNDI 0.966** 0.961** 0.924** 0.898* 0.883* 0.802* 0.937** PRI 0.969** 0.977** 0.931** 0.929** 0.759* 0.648 0.823* 说明：*表示P＜0.05，**表示P＜0.01
下载: 导出CSV
-
[1] 王啸晨. 4种观赏竹花叶色素组成、结构分析及相关调控基因的研究[D]. 北京: 中国林业科学研究院, 2012. WANG Xiaochen. Study on Pigment Composition, Structure of Variegated Leaf and Related Genes from Four Ornamental Bamboos[D]. Beijing: Chinese Academy of Forestry, 2012. [2] 杨海芸, 王晓芹, 张宁, 等. 日本花叶矢竹组织培养与叶色变异研究[J]. 竹子研究汇刊, 2010, 29(4): 15 − 20. YANG Haiyun, WANG Xiaoqin, ZHANG Ning, et al. Tissue culture and leaf color variation of Pseudosasa japonica cv. akebonosuji [J]. J Bamboo Res, 2010, 29(4): 15 − 20. [3] 王振兴, 曹建冉, 秦红艳, 等. 狗枣猕猴桃彩叶色素含量和结构共同影响叶色[J]. 植物生理学报, 2016, 52(12): 1921 − 1926. WANG Zhenxing, CAO Jianran, QIN Hongyan, et al. Common effect of pigment content and leaf structure on leaf color in Actinidia kolomikta [J]. Plant Physiol Commun, 2016, 52(12): 1921 − 1926. [4] 吴雪霞, 龚静, 查丁石. 遮荫对茄子幼苗叶片叶绿素荧光特性的影响[J]. 吉林蔬菜, 2010(4): 35 − 38. doi: 10.3969/j.issn.1672-0180.2010.04.015 WU Xuexia, GONG Jing, CHA Dingshi. Effects of shading on chlorophyll fluorescence characteristics in leaves of eggplant seedling [J]. Veg Jilin, 2010(4): 35 − 38. doi: 10.3969/j.issn.1672-0180.2010.04.015 [5] 陈凌艳, 何丽婷, 赖金莉, 等. 银丝竹不同叶色叶绿素合成及叶结构差异[J]. 森林与环境学报, 2017, 37(4): 385 − 391. CHEN Lingyan, HE Liting, LAI Jinli, et al. The variation of chlorophyll biosynthesis and the structure in different color leaves of Bambusa multiplex ‘Silverstripe’ [J]. J For Environ, 2017, 37(4): 385 − 391. [6] 刘儒, 原勤勤, 袁小平, 等. 不同枫香家系叶片色素含量变化及其与叶色变化的关系[J]. 南方林业科学, 2017, 45(4): 46 − 49. LIU Ru, YUAN Qinqin, YUAN Xiaoping, et al. The relationship with change of pigment content in leaves of different Liquidambar formosana families and change of leaf color [J]. Nanfang For Sci, 2017, 45(4): 46 − 49. [7] 成敏敏, 陈柯伊, 朱雪玉, 等. 花叶矢竹复绿期光合特性及叶绿体结构[J]. 林业科学, 2018, 54(4): 1 − 10. doi: 10.11707/j.1001-7488.20180401 CHENG Minmin, CHEN Keyi, ZHU Xueyu, et al. Photosynthetic characteristics and chloroplast ultrastructure of Pseudosasa japonica f.akebonosuji during green-revertible albino stage [J]. Sci Silv Sin, 2018, 54(4): 1 − 10. doi: 10.11707/j.1001-7488.20180401 [8] 张向娜, 熊立瑰, 温贝贝, 等. 茶树叶色变异研究进展[J]. 植物生理学报, 2020, 56(4): 643 − 653. ZHANG Xiangna, XIONG Ligui, WEN Beibei, et al. Advances in leaf color variation of tea plant (Camellia sinensis) [J]. J Plant Physiol, 2020, 56(4): 643 − 653. [9] 刘芬, 屈成, 肖楠, 等. 水稻高光谱变化特征与叶绿素含量监测研究[J]. 激光生物学报, 2017, 26(4): 326 − 333. doi: 10.3969/j.issn.1007-7146.2017.04.008 LIU Fen, QU Cheng, XIAO Nan, et al. A study on spectral characteristics and chlorophyll content in rice [J]. Acta Laser Biol Sin, 2017, 26(4): 326 − 333. doi: 10.3969/j.issn.1007-7146.2017.04.008 [10] JOLY D, BIGRAS C, HARNOIS J, et al. Kinetic analyses of the OJIP chlorophyll fluorescence rise in thylakoid membranes [J]. Photosynth Res, 2005, 84(1/3): 107 − 112. [11] ZHANG Shouren, GAO Rongfu. Effects of light stress on oxygen evolution and photochemical energy storage of hybrid poplar clones determined by photoacoustic technique [J]. Acta Bot Sin, 2000, 42(8): 818 − 823. [12] LICHTENTHALER H K. Chlorophylls and carotenoids: pigment photosynthetic biomembranes [J]. Methods Enzymol, 1987, 148C(1): 350 − 382. [13] 张宪政. 植物叶绿素含量测定——丙酮乙醇混合液法[J]. 辽宁农业科学, 1986(3): 26 − 28. ZHANG Xianzheng. Measurement of chlorophyll content in plants:acetone ethanol mixture [J]. Liaoning Agric Sci, 1986(3): 26 − 28. [14] GITELSON A A, MERZLYAK M N. Signature analysis of leaf reflectance spectra: algorithm development for remote sensing of chlorophyll [J]. J Plant Physiol, 1996, 148(3/4): 494 − 500. [15] GITELSON A A, GRITZ Y, MERZLYAK M N. Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves [J]. J Plant Physiol, 2003, 160(3): 271 − 282. doi: 10.1078/0176-1617-00887 [16] LIU Caifeng, GUO Jiali, CUI Yanlan, et al. Effects of cadmium and salicylic acid on growth, spectral reflectance and photosynthesis of castor bean seedlings [J]. Plant Soil, 2011, 344(1/2): 131 − 141. [17] GAMON, J A, SERRANO L, SURFUS J S. The photochemical reflectance index: an optical indicator of photosynthetic radiation use efficiency across species, functional types, and nutrient levels [J]. Oecologia, 1997, 112(4): 492 − 501. doi: 10.1007/s004420050337 [18] STRASSER R J, SRIVASTAVA A, TSIMILLI-MICHAE M. The fluorescence transient as a tool to characterize and screen photosynthetic samples[M]//YUNUS M, PATHRE U, MOHANTY P. Probing Photosynthesis: Mechansim, Regulation and Adaptation, London: Taylor and Francis Press, 2000: 445−483. [19] 李鹏民, 高辉远, STRASSER R J. 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J]. 植物生理与分子生物学学报, 2005, 31(6): 559 − 566. LI Pengmin, GAO Huiyuan, STRASSER R J. Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis [J]. Study J Plant Physiol Mol Biol, 2005, 31(6): 559 − 566. [20] RICHARDSON A D, DUIGAN S P, BERLYN G P. An evaluation of noninvasive methods to estimate foliar chlorophyll content [J]. New Phytol, 2010, 153(1): 185 − 194. [21] SCHANSKER G, SRIVASTAVA A, STRASSER R J. Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves [J]. Funct Plant Biol, 2003, 30(7): 785 − 796. doi: 10.1071/FP03032 [22] APPENROTH K J, STÖCKEL J, SRIVASTAVA A., et al. Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements [J]. Environ Pollut, 2001, 115(1): 49 − 64. doi: 10.1016/S0269-7491(01)00091-4 [23] 周云龙. 植物生物学[M]. 北京: 高等教育出版社, 1999. [24] 季鹏章, 梁名志, 宋维希, 等. 茶树珍稀品种‘紫娟’的叶片色素含量与叶色变化的关系研究[J]. 西南农业学报, 2010, 23(6): 1860 − 1862. doi: 10.3969/j.issn.1001-4829.2010.06.018 JI Pengzhang, LIANG Minzhi, SONG Weixi, et al. Relationship between changes of pigments content and leaf color changing in ‘Zijuan’ (Camellia sinensis var. assamica) [J]. Southwest China J Agric Sci, 2010, 23(6): 1860 − 1862. doi: 10.3969/j.issn.1001-4829.2010.06.018 [25] WANG Lu, YUE Chuan, CAO Hongli, et al. Biochemical and transcriptome analyses of a novel chlorophyll-deficient chlorina tea plant cultivar [J]. BMC Plant Biol, 2014, 14(1): 352. doi: 10.1186/s12870-014-0352-x [26] GITELSON A A, MERZLYAK M N, LICHTENTHALER H K. Detection of red edge position and chlorophyll content by reflectance measurements near 700 nm [J]. J Plant Physiol, 1996, 148(3/4): 501 − 508. [27] 李涛, 姜闯道. 密植对薄荷叶片光系统Ⅱ功能的影响[J]. 植物生理学报, 2017, 53(7): 1279 − 1286. LI Tao, JIANG Chuangdao. Effects of close planting on photosystem Ⅱ functions in Mentha haplocalyx leaves [J]. Plant Physiol Commun, 2017, 53(7): 1279 − 1286. [28] 孙小玲, 许岳飞, 马鲁沂, 等. 植株叶片的光合色素构成对遮阴的响应[J]. 植物生态学报, 2010, 34(8): 989 − 999. SUN Xiaoling, XU Yuefei, MA Luyi, et al. A review of acclimation of photosynthetic pigment composition in plant leaves to shade environment [J]. Chin J Plant Ecol, 2010, 34(8): 989 − 999. [29] 卢玉生, 官凤英, 彭超, 等. 竹笋截梢对绿竹生长及叶绿素荧光特性的影响[J]. 浙江农林大学学报, 2020, 37(1): 51 − 59. LU Yusheng, GUAN Fengying, PENG Chao, et al. Effects of bamboo shoot truncation on growth and chlorophyll fluorescence characteristics of Dendrocalamopsis oldhami [J]. J Zhejiang A&F Univ, 2020, 37(1): 51 − 59. [30] 韩涛. 叶绿素缺乏对水稻光系统光化学功能的影响[D]. 江苏: 南京农业大学, 2010.. HAN Tao. Effect of Chlorophyll Deficiency on Photosystem Photochemistry and Photoinhibition in Rice[D]. Nanjing: Nanjing Agricultural University, 2010. [31] 徐秀玉, 程来亮, 金立桥, 等. AOX途径在苹果离体叶片失水过程中的光破坏防御作用[J]. 西北植物学报, 2016, 36(5): 964 − 970. doi: 10.7606/j.issn.1000-4025.2016.05.0964 XU Xiuyu, CHENG Lailiang, JIN Liqiao, et al. Role of mitochondrial alternative oxidase(AOX) in photoprotection in apple detached leaf under water stress [J]. Acta Bot Boreali-Occident Sin, 2016, 36(5): 964 − 970. doi: 10.7606/j.issn.1000-4025.2016.05.0964 -
-
链接本文:
http://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.20200360
点击查看大图
计量
- 文章访问数: 19
- 被引次数: 0
