Advances in seed respiration detection and its application
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摘要: 种子呼吸反映了种子的多种内在属性和生理生化特征，种子呼吸检测方法的进展对种子呼吸代谢研究具有重要意义。本文从种子呼吸检测方法及其应用2个方面进行了综述，重点综述了小篮子法、瓦氏微量法、Clark氧电极法、红外线二氧化碳分析仪法、氧传感技术检测法(Q2技术)和可调谐二极管激光吸收光谱(TDLAS)技术检测法等常用种子呼吸检测方法的工作原理、主要检测对象及操作方法，总结了以上种子呼吸检测方法的优缺点及其适用范围。讨论了种子呼吸检测方法在种子呼吸代谢、种子储藏和种子活力测定等几个方面的研究应用，重点阐述了种子呼吸检测技术与种子活力相关的研究进展。根据研究现状对种子呼吸相关检测方法及其研究和应用方向进行了展望：①基于TDLAS技术等光学检测技术有望研制出灵敏度更高、操作更为简单的种子呼吸检测方法及装备；②种子呼吸代谢及其影响因素研究有利于丰富和深入揭示种子呼吸代谢过程理论；③开展种子呼吸指标和种子活力参数研究，为探寻能够将种子呼吸强度作为有效判定种子活力指标提供重要参数支持。表1参60Abstract: Seed respiration reflects a variety of internal properties and physiological and biochemical characteristics of seeds. The development of seed respiration detection methods is of great significance to the study of seed respiration metabolism. The methods of seed respiration detection and their application were reviewed in this paper. The focus of the review included working principle, main detection objects and operation principle of common seed respiration detection methods, such as small-skep-method, Warburg trace method, Clark oxygen electrode method, infrared CO2 analyzer method, oxygen sensing technology (Q2) and tunable diode laser absorption spectroscopy (TDLAS). The advantages, disadvantages and applicable scope of the above seed respiration detection methods were summarized. Secondly, the research and application of seed respiration detection methods in seed respiration metabolism, seed storage and seed vigor determination were discussed, with emphasis on the research progress of the correlation between seed respiration detection technology and seed vigor. Finally, based on the research status, seed respiration detection methods, their research and application direction were prospected: (1) It is expected to develop a seed respiration detection method and equipment with higher sensitivity and simpler operation based on optical detection technology such as TDLAS. (2) The study of seed respiration metabolism and its influencing factors is helpful to enrich and deeply reveal the theory of seed respiration metabolism. (3) Research on seed respiration index and seed vigor parameters should be carried out to provide important parameters to explore the possibility of using seed respiration intensity as an effective indicator to judge seed vigor. [Ch, 1 tab. 60 ref.]
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Key words:
- seed /
- seed respiration detection method /
- respiratory metabolism /
- seed storage /
- seed vigor
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表 1 种子呼吸检测方法比较
Table 1. Comparison of respiration detection methods for seeds
检测方法 检测原理 连续测量/
自动存储优点 缺点 预处理方法 检测时间 最少样本
检测量小篮子法[3] 化学 否 装置简单；应用范围广 易受外界环境干扰；反应
不敏感浸种、萌发 10 ~20 min 1批(约2 g) 瓦氏微量法[10] 化学/物理 否 灵敏度高；可多组同时
测定受温度影响大；难以用于
大粒种子测量浸种、萌发 10~20 min 1批(约1 g) Clark氧电极法[20] 电化学 是 响应快；灵敏度高 对温度敏感；需破碎种子 破碎、吸胀 10~20 min 1批 红外线CO2分析仪法[26] 光学 是 反应灵敏；检测精度高 价格昂贵；温度影响光源
稳定浸种、萌发 约5 s 1批(约1.5 g) 氧传感技术检测法[27] 化学 是 检测氧气，实现单粒种
子和多粒种子同步测量有荧光物质的消耗，无法
实时监测浸种、萌发 约30 min 单粒 TDLAS技术检测法[39] 光学 是 灵敏度高；分辨率高 尚在实验阶段；参数选择
对结果有影响清洗 约0.1 s 单粒 
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[1] MASYAGINA O V, PROKUSHKIN S G, SADILOVA M Y. Influence of temperature on fractional composition of proteins and respiration of germinating seeds of Gmelin and Siberian larch [J]. Contemp Probl Ecol, 2009, 2(6): 611 − 619. doi: 10.1134/S1995425509060198 [2] 杨忠仁, 郭霏, 张东, 等. 不同储藏年限沙葱种子萌发及呼吸生理的变化[J]. 西北农林科技大学学报(自然科学版), 2021, 49(11): 91 − 96, 114. YANG Zhongren, GUO Fei, ZHANG Dong, et al. Changes of germination and respiration physiology of Allium mongolicun seeds under different storage times [J]. J Northwest A&F Univ Nat Sci Ed, 2021, 49(11): 91 − 96, 114. [3] 张璇, 陈通旋, 郭江雪, 等. 不同激素对香果树种子发芽特性与呼吸速率的影响[J]. 贵州农业科学, 2019, 47(3): 116 − 118. doi: 10.3969/j.issn.1001-3601.2019.03.026 ZHANG Xuan, CHEN Tongxuan, GUO Jiangxue, et al. Effects of germination and respiration rate on Emmenopterys henryi seed with different hormones [J]. Guizhou Agric Sci, 2019, 47(3): 116 − 118. doi: 10.3969/j.issn.1001-3601.2019.03.026 [4] 李佳, 周素华, 贾娜, 等. 不同浓度赤霉素处理对杜仲种子萌发的影响[J]. 安徽农业科学, 2019, 47(3): 144 − 146, 185. doi: 10.3969/j.issn.0517-6611.2019.03.046 LI Jia, ZHOU Suhua, JIA Na, et al. Effects of different concentrations of gibberellin on seed germination in Eucommia ulmoides [J]. J Anhui Agric Sci, 2019, 47(3): 144 − 146, 185. doi: 10.3969/j.issn.0517-6611.2019.03.046 [5] 方能虎, 洪法水, 赵贵文. 稀土元素对水稻种子萌发初期呼吸速率动态变化的影响及其定位初探[J]. 稀土, 2000, 21(5): 41 − 43. doi: 10.3969/j.issn.1004-0277.2000.05.012 FANG Nenghu, HONG Fashui, ZHAO Guiwen. Effect of rare earth on dynamic change of respiratory rate and position-setting of rare earth ions in plants during early rice germination [J]. Chin Rare Earths, 2000, 21(5): 41 − 43. doi: 10.3969/j.issn.1004-0277.2000.05.012 [6] 杨雪鹏, 周留柱, 马科, 等. 维生素吡咯喹啉醌对水芹种子萌发的生理效应[J]. 轻工学报, 2019, 34(4): 1 − 7. doi: 10.3969/j.issn.2096-1553.2019.04.001 YANG Xuepeng, ZHOU Liuzhu, MA Ke, et al. Physiological effects of vitamin pyrroloquinoline quinone on the germination of cress seeds [J]. J Zhengzhou Univ Light Ind Nat Sci Ed, 2019, 34(4): 1 − 7. doi: 10.3969/j.issn.2096-1553.2019.04.001 [7] 李海霞, 徐久玮, 蔡明历, 等. 小篮子法测定植物种子呼吸速率的方法改进[J]. 生物学杂志, 2015, 32(1): 100 − 102, 106. doi: 10.3969/j.issn.2095-1736.2015.01.100 LI Haixia, XU Jiuwei, CAI Mingli, et al. Improved method for measuring respiratory rate of plant seeds using small-skep-method [J]. J Biol, 2015, 32(1): 100 − 102, 106. doi: 10.3969/j.issn.2095-1736.2015.01.100 [8] 吕洪飞, 余象煜, 李平. 杉木雄性不育株与可育株小孢子叶球及其叶的呼吸强度的比较研究[J]. 浙江师大学报(自然科学版), 1996, 19(2): 73 − 76. LÜ Hongfei, YU Xiangyu, LI Ping. Comparative studies on respiratory intensity of mierostrobilus and leaves between male sterile and fertile plants in Cunninghamia lanceolata [J]. J Zhejiang Norm Univ Nat Sci, 1996, 19(2): 73 − 76. [9] 黄真池, 黄上志. 不破坏种子活力测定方法研究(Ⅱ)种子活力与呼吸速率的关系[J]. 种子, 1998, 98(5): 3 − 5. HUANG Zhenchi, HUANG Shangzhi. Study on non-destructive method in seed vigor determination (Ⅱ) the relationship between respiratory rate and cabbage seed vigor [J]. Seed, 1998, 98(5): 3 − 5. [10] 黄学林, 陈润政. 种子生理实验手册[M]. 北京: 农业出版社, 1990: 101-107. HUANG Xuelin, CHEN Runzheng. Handbook of Seed Physiology[M]. Beijing: Agricultural Press, 1990: 101 − 107. [11] 王亚文, 袁俊杰, 马沐青, 等. 光照对黑豆种子萌发进程的影响研究[J]. 种子, 2017, 36(2): 99 − 102. WANG Yawen, YUAN Junjie, MA Muqing, et al. The effect of illumination on the germination process of black soybean seed [J]. Seed, 2017, 36(2): 99 − 102. [12] CLARK J. Monitor and control of blood and tissue oxygen tensions [J]. Trans-Am Soc Artif Intern Organs, 1956, 65(2): 41 − 48. [13] 朱建中. 微型Clark氧传感器的进展[J]. 传感器世界, 1996, 56(9): 5 − 10. ZHU Jianzhong. An important progress in the microminiaturization of Clark-type oxygen sensors [J]. Sens World, 1996, 56(9): 5 − 10. [14] NIETZEL T, MOSTERTZ J, RUBERTI C, et al. Redox-mediated Kick-Start of mitochondrial energy metabolism drives resource-efficient seed germination [J]. Proc Natl Acad Sci, 2019, 117(1): 741 − 751. [15] MEYER E H, WELCHEN E, CARRIE C. Assembly of the complexes of the oxidative phosphorylation system in land plant mitochondria [J]. Ann Rev Plant Biol, 2019, 70(1): 23 − 50. doi: 10.1146/annurev-arplant-050718-100412 [16] BENAMAR A, TALLON C, MACHEREL D. Membrane integrity and oxidative properties of mitochondria isolated from imbibing pea seeds after priming or accelerated ageing [J]. Seed Sci Res, 2003, 13(1): 35 − 45. doi: 10.1079/SSR2002122 [17] 王伟青, 程红焱, 刘树君, 等. 黄皮种子线粒体呼吸速率和活性氧清除酶活性对脱水的响应及其生态学意义[J]. 植物生态学报, 2012, 36(8): 870 − 879. WANG Weiqing, CHENG Hongyan, LIU Shujun, et al. Response of respiratory rate and reactive oxygen species scavenging enzyme activity in seed mitochondria of Clausena lansium dehydration and its ecological significance [J]. Chin J Plant Ecol, 2012, 36(8): 870 − 879. [18] 陶宗娅, 邹琦. 低温吸胀对大豆与豌豆种子呼吸代谢的影响[J]. 山东农业大学学报, 1993, 24(3): 247 − 252. TAO Zongya, ZOU Qi. Effect of imbibition under low temperature on respiratory metabolism of pea and soybean seeds [J]. J Shandong Agric Univ, 1993, 24(3): 247 − 252. [19] 李相贤, 徐亮, 高闽光, 等. 分析温室气体及CO2碳同位素比值的傅里叶变换红外光谱仪[J]. 光学精密工程, 2014, 22(9): 2359 − 2368. doi: 10.3788/OPE.20142209.2359 LI Xiangxian, XU Liang, GAO Minguang, et al. Fourier transform infrared greenhouse analyzer for gases and carbon isotope ratio [J]. Opt Precis Eng, 2014, 22(9): 2359 − 2368. doi: 10.3788/OPE.20142209.2359 [20] PATANÈ C, AVOLA G. A seed respiration-based index of cold-sensitivity during imbibition in four macrothermal species [J]. Acta Physiol Plant, 2013, 35(3): 911 − 918. doi: 10.1007/s11738-012-1134-4 [21] 段黎明, 孙学礼, 辛欣. 不分光红外线气体分析仪计量确认的实践[J]. 铁路节能环保与安全卫生, 2019, 9(4): 33 − 37. DUAN Liming, SUN Xueli, XIN Xin. Practice of non-spectral infrared gas analyzer metrological confirmation [J]. Railway Energy Saving Environ Prot Occupational Saf Health, 2019, 9(4): 33 − 37. [22] 陈润政, 蔡东燕, 傅家瑞. 花生种子生活力与呼吸强度及ATP含量的关系[J]. 中国油料, 1986, 35(1): 48 − 50. CHEN Runzheng, CAI Dongyan, FU Jiarui. Relationship between seed viability and respiration intensity and ATP content of peanut [J]. Chin J Oil Crop Sci, 1986, 35(1): 48 − 50. [23] 陈禅友, 童莉. 黄秋葵种子萌发生理特性分析[J]. 种子, 2004, 23(11): 14 − 17. doi: 10.3969/j.issn.1001-4705.2004.11.005 CHEN Chanyou, TONG Li. Analysis of physiologic characters of Okra seed germination [J]. Seed, 2004, 23(11): 14 − 17. doi: 10.3969/j.issn.1001-4705.2004.11.005 [24] 刘美. 山农17小麦种子萌发期间呼吸代谢相关酶活性及其基因表达量的变化[D]. 泰安: 山东农业大学, 2014. LIU Mei. Changes of Enzyme Activity and Gene Expression Related to Respiratory Metabolism During Shannong17 Wheat Seed Germination[D]. Tai’an: Shandong Agricultural University, 2014. [25] 陈能阜, 赵光武, 何勇, 等. 测定种子活力的新技术: Q2技术[J]. 种子, 2009, 28(12): 112 − 114. doi: 10.3969/j.issn.1001-4705.2009.12.038 CHEN Nengfu, ZHAO Guangwu, HE Yong, et al. Q2 Technology: a new technology for determinating seed vigor [J]. Seed, 2009, 28(12): 112 − 114. doi: 10.3969/j.issn.1001-4705.2009.12.038 [26] 陈合云. 基于氧传感技术的常规水稻种子质量检测方法研究[D]. 北京: 中国农业科学院, 2012. CHEN Heyun. Research on Method of Conventional Rice Seed Quality Determination Based on Oxygen Sensor Technology[D]. Beijing: Chinese Academy of Agricultural Sciences, 2012. [27] 潘威, 杜景诚, 乔雨, 等. 基于氧传感技术测定烟草种子活力的初步研究[J]. 种子, 2018, 37(6): 72 − 75. PAN Wei, DU Jingcheng, QIAO Yu, et al. Preliminary study on seed vigor testing of tobacco seeds based on oxygen sensing technology [J]. Seed, 2018, 37(6): 72 − 75. [28] TCHERKEZ G, CUI Jing, LAMADE E. Seed germination in oil palm (Elaeis guineensis Jacq.): a review of metabolic pathways and control mechanisms [J]. Int J Mol Sci, 2020, 21(12): 4227 − 4240. doi: 10.3390/ijms21124227 [29] HINKLEY E D. High-resolution infrared spectroscopy with a tunable diode laser [J]. Appl Phys Lett, 1970, 16(9): 351 − 354. doi: 10.1063/1.1653222 [30] REID J, SHEWCHUN J S, GARSIDE B K, et al. High sensitivity pollution detection employing tunable diode lasers [J]. Appl Opt, 1978, 17(2): 300 − 307. doi: 10.1364/AO.17.000300 [31] REID J, LABRIE D. Second harmonic detection with tunable diode lasers-comparison of experiment and theory [J]. Appl Phys B Lasers Opt, 1981, 26(3): 203 − 210. [32] HARTMANN A, STRZODA R, SCHROBENHAUSER R, et al. CO2 sensor for mainstream capnography based on TDLAS [J]. Appl Phys B Lasers Opt, 2014, 116(4): 1023 − 1026. doi: 10.1007/s00340-014-5791-4 [33] LIU Yu, XUE, Qingguo, ZUO Haibin, et al. Experimental study of H2 and/or N2 addition effects on CO/CO2: air flames using a combustion diagnostic system [J]. J Therm Sci, 2021, 30(4): 1 − 10. [34] YANG J, JIAO Y, YANG W Z, et al. Review of methods for determination of ammonia volatilization in farmland[J/OL]. IOP Conf Series Earth Environ Sci, 2018, 113[2021-10-05]. doi: 10.1088/1755-1315/113/1/012022. [35] HUNSMANN S, WUNDERLE K, WAGNER S, et al. Absolute, high resolution water transpiration rate measurements on single plant leaves via tunable diode laser absorpotion spectroscopy (TDLAS) at 1.37 μm [J]. Appl Phys B Lasers Opt, 2008, 92: 393 − 401. doi: 10.1007/s00340-008-3095-2 [36] ZHANG Rui, GUO Maoning, WANG Yizhong, et al. Research on CO2 detection system in refrigerated compartment of agricultural products based on TDLAS technology [J]. Proced CIRP, 2019, 83(12): 429 − 433. [37] 赵光武, 阮关海, 陈合云, 等. 氧传感技术及其在水稻种子活力检测中的应用与展望[J]. 中国稻米, 2011, 17(6): 44 − 46. doi: 10.3969/j.issn.1006-8082.2011.06.013 ZHAO Guangwu, RUAN Guanhai, CHEN Heyun, et al. Oxygen sensing technology and its application and prospect in rice seed vigor detection [J]. China Rice, 2011, 17(6): 44 − 46. doi: 10.3969/j.issn.1006-8082.2011.06.013 [38] 贾良权, 祁亨年, 胡文军, 等. 采用TDLAS技术的玉米种子活力快速无损分级检测[J]. 中国激光, 2019, 46(9): 289 − 297. JIA Liangquan, QI Hengnian, HU Wenjun, et al. Rapid nondestructive grading detection of maize seed vigor using TDLAS technique [J]. Chin J Lasers, 2019, 46(9): 289 − 297. [39] DALEY D O, CONSIDINE M J, HOWELL K A, et al. Respiratory gene expression in soybean cotyledons during post-germinative development [J]. Plant Mol Biol, 2003, 51(5): 745 − 755. doi: 10.1023/A:1022502501373 [40] SILVA A T, LIGTERINK W, HILHORST H W M. Metabolite profiling and associated gene expression reveal two metabolic shifts during the seed-to-seeding transition in Arabidopsis thaliana [J]. Plant Mol Biol, 2017, 95(4): 481 − 496. [41] ROSENTAL L, NONOGAKI H, FAIT A. Activation and regulation of primary metabolism during seed germination [J]. Seed Sci Res, 2014, 24(1): 1 − 15. doi: 10.1017/S0960258513000391 [42] DOMERGUE J-B, ABADIE C, LIMAMI A M, et al. Seed quality and carbon primary metabolism [J]. Plant Cell Environ, 2019, 42(10): 2776 − 2788. doi: 10.1111/pce.13618 [43] 郭聪聪, 沈永宝, 史锋厚. 白皮松种子萌发过程中呼吸代谢和内源激素对温度变化的响应[J]. 中南林业科技大学报, 2021, 41(3): 25 − 36. GUO Congcong, SHEN Yongbao, SHI Fenghou. Response of respiration and hormones during germination of Pinus bungeana seeds to temperature changes [J]. J Central South Univ ForTechnol, 2021, 41(3): 25 − 36. [44] SIMMONDS J A, SIMPSON G M. Increased participation of pentose phosphate pathway in response to afterripening and gibberellic acid treatment in caryopses of Avena fatua [J]. J Can Bot, 1971, 49(10): 1833 − 1840. doi: 10.1139/b71-258 [45] 浦心春, 韩建国, 李敏, 等. 结缕草种子打破休眠过程呼吸途径的研究[J]. 草业学报, 1996, 5(3): 57 − 61. PU Xinchun, HAN Jianguo, LI Min, et al. Studies on respiratory pathways of zoysiagrass seed when breaking dormancy [J]. Acta Pratacultural Sin, 1996, 5(3): 57 − 61. [46] 陈丽培, 王国霞, 沈永宝. 油松种子萌发初始阶段的呼吸代谢[J]. 林业科学, 2012, 48(5): 150 − 153. doi: 10.11707/j.1001-7488.20120523 CHEN Lipei, WANG Guoxia, SHEN Yongbao. Respiratory metabolism of Pinus tabulaeformis seeds at the initial germinating stages [J]. Sci Silv Sin, 2012, 48(5): 150 − 153. doi: 10.11707/j.1001-7488.20120523 [47] 管雨, 贾文庆, 刘会超, 等. 木瓜花粉生活力测定及储藏特性[J]. 浙江农林大学学报, 2012, 29(5): 790 − 794. doi: 10.11833/j.issn.2095-0756.2012.05.024 GUAN Yu, JIA Wenqing, LIU Huichao, et al. Pollen viability and storage of Chaenomeles sinensis [J]. J Zhejiang A&F Univ, 2012, 29(5): 790 − 794. doi: 10.11833/j.issn.2095-0756.2012.05.024 [48] 陈志超, 黄婷婷, 王洋, 等. 水稻种子休眠特性与耐藏性的关系[J]. 浙江农林大学学报, 2018, 35(3): 490 − 496. doi: 10.11833/j.issn.2095-0756.2018.03.013 CHEN Zhichao, HUANG Tingting, WANG Yang, et al. Dormancy characteristics and seed storage tolerance in rice [J]. J Zhejiang A&F Univ, 2018, 35(3): 490 − 496. doi: 10.11833/j.issn.2095-0756.2018.03.013 [49] 胡小荣, 陶梅, 卢新雄, 等. 超干燥种子储藏于不同温度下的呼吸作用及乙烯释放量的研究[J]. 种子, 2006, 25(2): 13 − 16. doi: 10.3969/j.issn.1001-4705.2006.02.005 HU Xiaorong, TAO Mei, LU Xinxiong, et al. Studies on the respiration and ethylene released amount of ultradried seed stored at different temperature [J]. Seed, 2006, 25(2): 13 − 16. doi: 10.3969/j.issn.1001-4705.2006.02.005 [50] LIU Sheng, ZHANG Hongli, ZHENG Shufang. Effect of pressure precooling, room pre-cooling and cold storage on quality of sweet corn [J]. Acta Hortic, 2012, 934(2): 1247 − 1254. [51] 王道营, 诸永志, 曹建民, 等. 储藏温度对甜玉米品质的影响[J]. 江西农业学报, 2008, 20(6): 82 − 83. doi: 10.3969/j.issn.1001-8581.2008.06.026 WANG Daoying, ZHU Yongzhi, CAO Jianmin, et al. Effects of storage temperature on quality of sweet maize [J]. Acta Agric Jiangxi, 2008, 20(6): 82 − 83. doi: 10.3969/j.issn.1001-8581.2008.06.026 [52] 王若兰, 严佳, 李燕羽, 等. 不同条件下小麦呼吸速率变化的研究[J]. 河南工业大学学报(自然科学版), 2009, 30(4): 12 − 16. WANG Ruolan, YAN Jia, LI Yanyu, et al. Research on the respiratory rate change of wheat under different conditions [J]. J Henan Univ Tec Nat Sci, 2009, 30(4): 12 − 16. [53] 王润豪, 陈红卫, 吴俊, 等. 水氮互作对百农207种子发芽特性及储藏物质转运的影响[J]. 麦类作物学报, 2019, 39(1): 82 − 88. doi: 10.7606/j.issn.1009-1041.2019.01.11 WANG Runhao, CHEN Hongwei, WU Jun, et al. Effects of water-nitrogen interaction on seed germination characteristics and storage substance transport in Bainong 207 [J]. J Triticeae Crops, 2019, 39(1): 82 − 88. doi: 10.7606/j.issn.1009-1041.2019.01.11 [54] ARAUJO A C F B, BARBEDO C. Changes in desiccation tolerance and respiratory rates of immature Caesalpinia echinata Lam. seeds [J]. J Seed Sci, 2017, 39(2): 123 − 132. doi: 10.1590/2317-1545v39n2167788 [55] 徐振飞, 过晟鹏, 钱旺, 等. 3种杉木种子活力测定方法比较[J]. 浙江农林大学学报, 2020, 37(6): 1230 − 1234. doi: 10.11833/j.issn.2095-0756.20190615 XU Zhenfei, GUO Shengpeng, QIAN Wang, et al. A comparative study of three determination methods for seed vigor of Cunninghamia lanceolata [J]. J Zhejiang A&F Univ, 2020, 37(6): 1230 − 1234. doi: 10.11833/j.issn.2095-0756.20190615 [56] 袁俊, 郑雯, 祁亨年, 等. 种子活力光学无损检测技术研究进展[J]. 作物杂志, 2020(5): 9 − 16. YUAN Jun, ZHENG Wen, QI Hengnian, et al. Progress in research of optical non-destructive test technology for seed vigor [J]. Crops, 2020(5): 9 − 16. [57] 胡元森, 田萍萍, 李棒棒, 等. 粮食储藏生理性变质研究进展[J]. 中国粮油学报, 2020, 35(11): 179 − 186. doi: 10.3969/j.issn.1003-0174.2020.11.028 HU Yuansen, TIAN Pingping, LI Bangbang, et al. Progress in physiological deterioration of stored grain [J]. J Chin Cereals Oils Assoc, 2020, 35(11): 179 − 186. doi: 10.3969/j.issn.1003-0174.2020.11.028 [58] 秦宏鹏, 刘翔宇, 陈娟. 溶解氧多参数智能补偿校正检测技术的研究[J]. 北京化工大学学报(自然科学版), 2019, 46(3): 99 − 104. QIN Hongpeng, LIU Xiangyu, CHEN Juan. Multi-parameter intelligent compensation correction detection technology for determination of dissolved oxygen [J]. J Beijing Univ Chem Tec Nat Sci, 2019, 46(3): 99 − 104. [59] WOODSTOCK L W, GRABE D F. Relationships between seed respiration during imbibition and subsequent seedling growth in Zea mays L. [J]. Plant Physiol, 1967, 42: 1071 − 1076. doi: 10.1104/pp.42.8.1071 [60] 钟希琼, 林丽超, 上官国莲. 水稻老化种子活力与生理性状关系的研究[J]. 佛山科学技术学院学报(自然科学版), 2003, 21(1): 64 − 66. ZHONG Xiqiong, LIN Lichao, SHANGGUAN Guolian. Study on the correlation between physiological characters and vigor of aged rice seed [J]. J Foshan Univ Nat Sci, 2003, 21(1): 64 − 66. -
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