Optimum conditions for rapid propagation with clones of Acacia implexa
-
摘要: 灰木相思Acacia implexa是南方优良的多用途造林树种,但种子繁殖性状分离严重,试图通过组织培养找到新的繁育途径。通过对灰木相思成年优树幼嫩的带腋芽茎段的离体培养试验,探讨了灰木相思茎段不定芽分化、继代增殖和壮苗生根的最佳培养条件。采用正交试验对防止培养过程中出现玻璃化苗的方法进行了探讨。结果表明:适宜灰木相思不定芽诱导的培养基配方为MS (Murashige and Skoog)+1.0 mg·L-16-苄氨基嘌呤(6-BA)+0.2 mg·L-1萘乙酸(NAA)+1.0 mg·L-1玉米素(ZT),适宜继代培养的培养基为改良1/2 MS (NH+4:NO-3=1:2)+1.0 mg·L-16-BA+0.05 mg·L-1NAA,适宜进行壮苗生根的培养基配方为改良1/2 MS+0.1 mg·L-1 NAA,移栽后成活率可达70%。Abstract: Acacia implexa is an excellent multipurpose tree species in South China, however seed propagation will cause segregation of inheritable characteristics and then, limit its utilization. This study looked at the use of tissue culture to clone trees with three plant growth regulators (6-BA; NAA, ZT) in different levels, and appropriate methods for possible ways to prevent vitrification of adventitious buds by orthogonal experiment L9(34). Results of experiments on micropropagation of young stem sections for selected adult trees of A. implexa showed that a suitable medium for adventitious bud differentiation of the stem sections with axillary buds was:MS (Murashige and Skoog's medium)+1.0 mg·L-1 BA(6-benzyladenine) +0.2 mg·L-1 NAA(α-naphthalene acetic acid)+1.0 mg·L-1 ZT(zeatin), a suitable medium for adventitious bud multiplication was:1/2 MS(NH+4:NO-3=1:2)+1.0 mg·L-16-BA+0.05 mg·L-1 NAA, and a suitable medium for improving roots was:1/2 MS+0.1 mg·L-1 NAA. After transplanting, over 70% of the tube plantlets survived.
-
Key words:
- botany /
- Acacia implexa /
- in vitro culture
-
[1] 潘志刚,游应天,李玉科,等.中国主要外来树种引种栽培[M].北京:北京科学技术出版社,1994:378-406. [2] 周鸿彬,高本旺,高登梅,等.相思类树种引种初报[J].湖北林业科技, 2001(4):19-22. [3] 詹夷生.闽北耐寒相思种源选择研究初报[J].福建林学院学报,1999,19(2):153-156. [4] 陈桂芳,娄利华.银荆相思组织培养及快繁技术研究[J].西南农业大学学报:自然科学版,2004,26(2):195-197. [5] 洪伟.林业试验设计技术与方法[M].北京:北京科学技术出版社,1993:148-159. [6] 蔡玲,王以红,吴幼媚,等. 5种相思树组织培养研究[J].广西林业科学,2003,32(1):24-26. [7] 张宏伟,黄学林,傅家瑞.大叶相思、马占相思腋芽培养和植株再生[J].热带亚热带植物学报,1995,3(3):62-68. [8] 苏秀城.黑木相思组织培养的初步研究[J].福建林业科技,1999,26(4):78-81. [9] 蒋泽平,梁珍海,汪有良.苦楝优良无性系试管苗玻璃化的影响因素[J].浙江林学院学报,2006,23(4):420-423. [10] 朱玉球,廖望仪.山核桃愈伤组织诱导的初步研究[J].浙江林学院学报, 2001,18(2):115-118. [11] 王碧琴,余发新,刘腾云.木兰科7种植物的组织培养技术研究[J].江西农业大学学报, 2006,28(2):268-273. -
链接本文:
https://zlxb.zafu.edu.cn/article/id/735f4e68-b638-45a2-b1a4-2b5cab3d5d74
计量
- 文章访问数: 600
- HTML全文浏览量: 55
- PDF下载量: 18
- 被引次数: 0