土壤中山核桃干腐病抑制菌的筛选和鉴定

田甜; 沈振明; 徐秋芳; 刘卜榕

doi:10.11833/j.issn.2095-0756.2012.01.011

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土壤中山核桃干腐病抑制菌的筛选和鉴定

田甜 , 沈振明 , 徐秋芳 , 刘卜榕

文章导航 > 浙江农林大学学报 > 2012 > 29(1): 58-64

田甜, 沈振明, 徐秋芳, 刘卜榕. 土壤中山核桃干腐病抑制菌的筛选和鉴定[J]. 浙江农林大学学报, 2012, 29(1): 58-64. doi: 10.11833/j.issn.2095-0756.2012.01.011

引用本文:

田甜, 沈振明, 徐秋芳, 刘卜榕. 土壤中山核桃干腐病抑制菌的筛选和鉴定[J]. 浙江农林大学学报, 2012, 29(1): 58-64. doi: 10.11833/j.issn.2095-0756.2012.01.011

TIAN Tian, SHEN Zhen-ming, XU Qiu-fang, LIU Bo-rong. Screening and identification of inhibiting strains for Carya cathayensis canker disease[J]. Journal of Zhejiang A&F University, 2012, 29(1): 58-64. doi: 10.11833/j.issn.2095-0756.2012.01.011

Citation:

TIAN Tian, SHEN Zhen-ming, XU Qiu-fang, LIU Bo-rong. Screening and identification of inhibiting strains for Carya cathayensis canker disease[J]. Journal of Zhejiang A&F University, 2012, 29(1): 58-64. doi: 10.11833/j.issn.2095-0756.2012.01.011

土壤中山核桃干腐病抑制菌的筛选和鉴定

doi: 10.11833/j.issn.2095-0756.2012.01.011

1.
浙江农林大学环境与资源学院，浙江临安 311300;
2.
浙江省临安市林业技术服务总站，浙江临安 311300

详细信息

通信作者: 徐秋芳

Screening and identification of inhibiting strains for Carya cathayensis canker disease

1.
School of Environmental and Resource Sciences，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;
2.
Forest Technology Service General Station of Lin’an City，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: XU Qiu-fang

摘要: 为了从土壤中筛选具有抑制山核桃Carya cathayensis干腐病菌的微生物，运用平皿对峙法从土壤中初筛得到对山核桃干腐病菌葡萄座腔菌Botryosphaeria dothidea具有拮抗作用的菌株5株，对峙培养发现菌株QZ2和QZ8抑菌效果显著，对峙试验显示QZ2，QZ8与病原菌之间有明显的抑菌圈，且QZ2孢子能完全覆盖在病原菌上生长而病原菌生长停止。通过形态特征观察及ITS 序列分析，菌株QZ2被鉴定为棘孢木霉Trichoderma asperellum，主要通过重寄生以达到控病的效果；菌株QZ8为草酸青霉Penicillium oxalicum，主要通过代谢产生的抗菌素起作用。QZ2 和QZ8是山核桃干腐病的潜在生防菌。图7参13
- 森林保护学 /
- 山核桃干腐病 /
- 抑制菌 /
- 筛选鉴定 /
- ITS序列分析
Abstract: The objective of this paper was to get a few of available inhibiting strains against Carya cathayensis canker disease. Inhibition assay was used to preliminarily screen strains from soils that inhibited Botryosphaeria dothidea，the pathogen of Carya cathayensis canker disease. Then，phenotypic characteristics and analysis of internal transcribed spacer （ITS） fragments were used to identify the strains. Results of the preliminary screening revealed five strains. After being cultured against pathogen，two of five strains，QZ2 and QZ8，exhibited inhibiting capability with apparent inhibition zones between the pathogen and strains QZ2 and QZ8 being observed. Spores of strain QZ2 finally covered the pathogen completely with pathogen growth ceasing. QZ2，which controlled the canker disease by parasitizing the disease strain，was identified as Trichoderma asperellum. Strain QZ8，which inhibited disease pathogen growth by creating antibiotics，was identified as Penicillium oxalicum. It can be concluded that QZ2 and QZ8 are potential strains for development of bio-controller against Carya cathayensis canker disease. ［Ch，7 fig. 13 ref.］
- forest protection /
- Carya cathayensis canker disease /
- inhibiting strain /
- screening and identification /
- internal transcribed spacer （ITS） sequence analysis

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土壤中山核桃干腐病抑制菌的筛选和鉴定

doi: 10.11833/j.issn.2095-0756.2012.01.011

1. 浙江农林大学环境与资源学院，浙江临安 311300;

2. 浙江省临安市林业技术服务总站，浙江临安 311300

通信作者: 徐秋芳

收稿日期: 2011-04-22
修回日期: 2011-05-20
刊出日期: 2012-02-20

关键词:

摘要: 为了从土壤中筛选具有抑制山核桃Carya cathayensis干腐病菌的微生物，运用平皿对峙法从土壤中初筛得到对山核桃干腐病菌葡萄座腔菌Botryosphaeria dothidea具有拮抗作用的菌株5株，对峙培养发现菌株QZ2和QZ8抑菌效果显著，对峙试验显示QZ2，QZ8与病原菌之间有明显的抑菌圈，且QZ2孢子能完全覆盖在病原菌上生长而病原菌生长停止。通过形态特征观察及ITS 序列分析，菌株QZ2被鉴定为棘孢木霉Trichoderma asperellum，主要通过重寄生以达到控病的效果；菌株QZ8为草酸青霉Penicillium oxalicum，主要通过代谢产生的抗菌素起作用。QZ2 和QZ8是山核桃干腐病的潜在生防菌。图7参13

English Abstract

Screening and identification of inhibiting strains for Carya cathayensis canker disease

1. School of Environmental and Resource Sciences，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Forest Technology Service General Station of Lin’an City，Lin’an 311300，Zhejiang，China

Corresponding author: XU Qiu-fang

Received Date: 2011-04-22
Rev Recd Date: 2011-05-20
Publish Date: 2012-02-20

Keywords:

Abstract: The objective of this paper was to get a few of available inhibiting strains against Carya cathayensis canker disease. Inhibition assay was used to preliminarily screen strains from soils that inhibited Botryosphaeria dothidea，the pathogen of Carya cathayensis canker disease. Then，phenotypic characteristics and analysis of internal transcribed spacer （ITS） fragments were used to identify the strains. Results of the preliminary screening revealed five strains. After being cultured against pathogen，two of five strains，QZ2 and QZ8，exhibited inhibiting capability with apparent inhibition zones between the pathogen and strains QZ2 and QZ8 being observed. Spores of strain QZ2 finally covered the pathogen completely with pathogen growth ceasing. QZ2，which controlled the canker disease by parasitizing the disease strain，was identified as Trichoderma asperellum. Strain QZ8，which inhibited disease pathogen growth by creating antibiotics，was identified as Penicillium oxalicum. It can be concluded that QZ2 and QZ8 are potential strains for development of bio-controller against Carya cathayensis canker disease. ［Ch，7 fig. 13 ref.］

田甜, 沈振明, 徐秋芳, 刘卜榕. 土壤中山核桃干腐病抑制菌的筛选和鉴定[J]. 浙江农林大学学报, 2012, 29(1): 58-64. doi: 10.11833/j.issn.2095-0756.2012.01.011

引用本文:

田甜, 沈振明, 徐秋芳, 刘卜榕. 土壤中山核桃干腐病抑制菌的筛选和鉴定[J]. 浙江农林大学学报, 2012, 29(1): 58-64. doi: 10.11833/j.issn.2095-0756.2012.01.011

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土壤中山核桃干腐病抑制菌的筛选和鉴定

doi: 10.11833/j.issn.2095-0756.2012.01.011

通信作者: 徐秋芳

Screening and identification of inhibiting strains for Carya cathayensis canker disease

Corresponding author: XU Qiu-fang

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出版历程

土壤中山核桃干腐病抑制菌的筛选和鉴定

doi: 10.11833/j.issn.2095-0756.2012.01.011

1. 浙江农林大学 环境与资源学院，浙江 临安 311300; 2. 浙江省临安市林业技术服务总站，浙江 临安 311300

通信作者: 徐秋芳

English Abstract

Screening and identification of inhibiting strains for Carya cathayensis canker disease

1. School of Environmental and Resource Sciences，Zhejiang A & F University，Lin’an 311300，Zhejiang，China; 2. Forest Technology Service General Station of Lin’an City，Lin’an 311300，Zhejiang，China

Corresponding author: XU Qiu-fang

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目录

1. 浙江农林大学环境与资源学院，浙江临安 311300;

2. 浙江省临安市林业技术服务总站，浙江临安 311300

1. School of Environmental and Resource Sciences，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Forest Technology Service General Station of Lin’an City，Lin’an 311300，Zhejiang，China