Chlorophyll fluorescence characteristics with simulated acid rain and light intensity treatments for Dicranopteris dichotoma
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摘要: 以盆栽芒萁Dicranopteris dichotoma为研究对象,通过人工遮阳及设置不同pH值的模拟酸雨复合处理的方式,探讨4种光环境(全光,遮光30%,遮光50%,遮光80%)和4种酸液(pH 3.0,pH 4.0,pH 5.6,pH 7.0)下芒萁色素质量分数及叶绿素荧光参数的差异。结果表明:①pH 4.0和pH 5.6处理提高了芒萁PSⅡ实际光化学效率(Yield),PSⅡ光化学效率(Fv /Fm),PSⅡ潜在活性(Fv /Fo)以及叶绿素a,叶绿素b,总叶绿素(a+b)和类胡萝卜素的质量分数,pH 3.0处理降低了荧光参数和色素质量分数。②随着遮光程度的增加,芒萁荧光参数Yield,Fv /Fm和 Fv /Fo值增大,Yield在80%和30%遮光处理间均有显著差异(P<0.05),叶绿素a,叶绿素b,总叶绿素(a+b)和类胡萝卜素的质量分数亦呈现显著性增大趋势(P<0.05)。同时光照强度对芒萁色素质量分数和荧光参数的影响大于酸雨胁迫,且无显著性交互作用。因此,在3种遮光处理下,pH 5.6和pH 4.0的酸液对芒萁叶绿素质量分数及荧光参数有促进作用;而在同一酸液处理下,随遮光程度增加芒萁叶绿素质量分数及荧光参数值趋于增大。图3表2参25Abstract: The effects of a light intensity gradient and acid rain on chlorophyll fluorescence parameters and pigment content of Dicranopteris dichotoma were studied using treatments of four different light gradients (shading of 80%, 50%, 30%, and full sun) and pH levels (pH 3.0, pH 4.0, pH 5.6 and pH 7.0). Results showed that with pH 4.0 and pH 5.6 treatments, the actual photochemistry efficiency of PS Ⅱ(Yield), photochemistry efficiency of PS Ⅱ(Fv /Fm), potential activity of PS Ⅱ(Fv /Fo), chlorophyll a (Chla), chlorophyll b (Chlb), total chlorophyll (Ct), and carotenoid (Car) content of D. dichotoma increased; whereas these parameters decreased with the pH 3.0 treatment. The chlorophyll fluorescence parameters Yield, Fv /Fm, and Fv /Foincreased with shading. Yield with 80% shading was significantly than 30% shading (P<0.05). Chla, Chlb, Ct, and Car content were also significantly greater (P<0.05). For pigment content and fluorescence parameters, the effects of light intensity and acid rain stress had no significant interactions, and light intensity had a significantly greater effect on the parameters than acid rain stress. Therefore, acidic liquids of pH 5.6 and pH 4.0 with shading of 80%, 50%, and 30% promoted chlorophyll content and fluorescence parameters, and as the degree of shading increased the chlorophyll content and chlorophyll fluorescence parameters also increased.[Ch, 3 fig. 2 tab. 25 ref.]
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Key words:
- botany /
- Dicranopteris dichotoma /
- simulated acid rain /
- shading /
- chlorophyll fluorescence /
- pigment
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2015.01.008
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