Volume 27 Issue 2
Apr.  2010
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JIANG Yan-feng, SI Jin-ping, HUANG Hua-hong, CHENG Long-jun. An AFLP molecular labeling technique for Magnolia officinalis[J]. Journal of Zhejiang A&F University, 2010, 27(2): 304-309. doi: 10.11833/j.issn.2095-0756.2010.02.024
Citation: JIANG Yan-feng, SI Jin-ping, HUANG Hua-hong, CHENG Long-jun. An AFLP molecular labeling technique for Magnolia officinalis[J]. Journal of Zhejiang A&F University, 2010, 27(2): 304-309. doi: 10.11833/j.issn.2095-0756.2010.02.024

An AFLP molecular labeling technique for Magnolia officinalis

doi: 10.11833/j.issn.2095-0756.2010.02.024
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  • Corresponding author: SI Jin-ping
  • Publish Date: 2010-04-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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An AFLP molecular labeling technique for Magnolia officinalis

doi: 10.11833/j.issn.2095-0756.2010.02.024
    Corresponding author: SI Jin-ping

Abstract: To establish an efficient and stable amplified fragment length polymorphism (AFLP) analysis system for Magnolia officinalis,selection and optimization of DNA template treatments and selective amplification conditions were undertaken using the cetyl trimethylammonium bromide (CTAB)-SiO2 method for mapping. Results indicated that: High quality DNA samples were obtained with the CTAB-SiO2 method and the DNA template used was 400 ng. The best treatments for the analysis were Mg2+ of 2.00 mmolL-1,deoxyribonucleotide triphosphates(dNTPs) of 0.90 mmolL-1,and Taq DNA polymerase of 16.67 nkat. This research showed that the CTAB-SiO2 method was fit for AFLP analysis on Magnolia,that a 30 times dilution for the pre-amplification products could be used for selective amplification,and that the silver-staining AFLP system could provide a method for further study with molecular markers in the field of genetic diversity,for breeding new cultivars,and for genetic relationships with Magnolia officinalis.[Ch,7 fig. 1 tab. 12 ref.]

JIANG Yan-feng, SI Jin-ping, HUANG Hua-hong, CHENG Long-jun. An AFLP molecular labeling technique for Magnolia officinalis[J]. Journal of Zhejiang A&F University, 2010, 27(2): 304-309. doi: 10.11833/j.issn.2095-0756.2010.02.024
Citation: JIANG Yan-feng, SI Jin-ping, HUANG Hua-hong, CHENG Long-jun. An AFLP molecular labeling technique for Magnolia officinalis[J]. Journal of Zhejiang A&F University, 2010, 27(2): 304-309. doi: 10.11833/j.issn.2095-0756.2010.02.024

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