Mycorrhiza formation and acclimatization of tissue cultured <em>Pinus elliottii</em>

ZHU Li-hua; CHENG Fang; QIN Li; YE Jian-ren

doi:10.11833/j.issn.2095-0756.2010.03.013

Volume 27 Issue 3

Jun. 2010

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ZHU Li-hua, CHENG Fang, QIN Li, YE Jian-ren. Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii[J]. Journal of Zhejiang A&F University, 2010, 27(3): 398-403. doi: 10.11833/j.issn.2095-0756.2010.03.013

Citation:

ZHU Li-hua, CHENG Fang, QIN Li, YE Jian-ren. Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii[J]. Journal of Zhejiang A&F University, 2010, 27(3): 398-403. doi: 10.11833/j.issn.2095-0756.2010.03.013

Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii

doi: 10.11833/j.issn.2095-0756.2010.03.013

1.
Jiangsu Key Laboratory for Prevention and Management of Invasasion Species，College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

More Information

Corresponding author: YE Jian-ren
Publish Date: 2010-06-20

Abstract

The condition for axillary bud induction of Pinus elliottii has been optimized，and mycorrhizas on the rooted explants has been tested in vitro to improve the efficiency of micropropagation. Axillary bud induction was found to be influenced by the basal medium as well as the age of the seedling from which the explant was taken. Modified Gresshoff and Doys（GD） medium proved to be superior to others，and the right stage of seedling was 18 － 25 d. The addition of activated charcoal（0.5 mgL－1） stimulated shoot elongation. Rooting occured at about 80% on 1/2GD medium supplemented with 0.05 mgL－1 NAA and 1.0 mgL－1 IBA. Mycorrhizas were initiated for plantlets with Pisolithus tinctorius on an agar medium under fully controlled conditions. Mycorrhizas improved the transfer of the plantlets to ex vitro conditions. After 3 years in the field，the plantlets reached an average of 80.3 cm in height. Their shoot growth appearance was normal.［Ch，2 fig. 4 tab. 28 ref.］
- silviculture,
- Pinus elliottii,
- micropropagation,
- adventitious root,
- Pisolithus tinctorius,
- mycorrhiza formation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii

1. Jiangsu Key Laboratory for Prevention and Management of Invasasion Species，College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

Corresponding author: YE Jian-ren

Publish Date: 2010-06-20

Key words:

Abstract: The condition for axillary bud induction of Pinus elliottii has been optimized，and mycorrhizas on the rooted explants has been tested in vitro to improve the efficiency of micropropagation. Axillary bud induction was found to be influenced by the basal medium as well as the age of the seedling from which the explant was taken. Modified Gresshoff and Doys（GD） medium proved to be superior to others，and the right stage of seedling was 18 － 25 d. The addition of activated charcoal（0.5 mgL－1） stimulated shoot elongation. Rooting occured at about 80% on 1/2GD medium supplemented with 0.05 mgL－1 NAA and 1.0 mgL－1 IBA. Mycorrhizas were initiated for plantlets with Pisolithus tinctorius on an agar medium under fully controlled conditions. Mycorrhizas improved the transfer of the plantlets to ex vitro conditions. After 3 years in the field，the plantlets reached an average of 80.3 cm in height. Their shoot growth appearance was normal.［Ch，2 fig. 4 tab. 28 ref.］

Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii

doi: 10.11833/j.issn.2095-0756.2010.03.013

Abstract

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通讯作者: 陈斌, bchen63@163.com

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Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii

doi: 10.11833/j.issn.2095-0756.2010.03.013

1. Jiangsu Key Laboratory for Prevention and Management of Invasasion Species，College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

Corresponding author: YE Jian-ren

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Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii

doi: 10.11833/j.issn.2095-0756.2010.03.013

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Mycorrhiza formation and acclimatization of tissue cultured Pinus elliottii

doi: 10.11833/j.issn.2095-0756.2010.03.013

1. Jiangsu Key Laboratory for Prevention and Management of Invasasion Species，College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

Corresponding author: YE Jian-ren

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