Separation，purification，and identification of endogenous inhibitory substances in <em>Pinus massoniana</em> cuttings

LI Yong-jin; DING Gui-jie

doi:10.11833/j.issn.2095-0756.2010.04.005

Volume 27 Issue 4

Aug. 2010

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LI Yong-jin, DING Gui-jie. Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings[J]. Journal of Zhejiang A&F University, 2010, 27(4): 507-512. doi: 10.11833/j.issn.2095-0756.2010.04.005

Citation:

LI Yong-jin, DING Gui-jie. Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings[J]. Journal of Zhejiang A&F University, 2010, 27(4): 507-512. doi: 10.11833/j.issn.2095-0756.2010.04.005

Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings

doi: 10.11833/j.issn.2095-0756.2010.04.005

LI Yong-jin^1,2,
DING Gui-jie^1
,

1.
The Institute of Silviculture and Ecology，Guizhou University，Guiyang 550025，Guizhou，China;
2.
Hunan Forestry Academy，Changsha 410004，Hunan，China

More Information

Corresponding author: DING Gui-jie
Publish Date: 2010-08-20

Abstract

To improve the rooting rate of Pinus massoniana，endogenous inhibitors from cuttings of five P. massoniana lines were purified and separated using extraction and high pressure liquid chromatography （HPLC）. Biological assays along with analysis of variance （ANOVA） with 4 treatments of aether extracts and 3 replications with comparisons to Brassica chinensis seeds were employed. Results revealed seven components of endogenous inhibitors in each P. massoniana cutting belonging to flavones，phenols，and abscisic acid（ABA）. This indicated that their expression was controlled by genes with significant differences among lines（P = 0.032 9）. By comparison，an ANOVA for the influence of endogenous inhibitors on growth of B. chinensis roots was highly significant （P = 0.000 1）；whereas compared to the same component of endogenous inhibitors in the root growth of B. chinensis，inhibition with the five P. massoniana lines were not significantly different. Also，except for MI6（No. 6 of HLPC educts），correlation analyses between the relative content of endogenous inhibitors and the rooting rate of B. chinensis were negative. Biological assays for extractions and their residuals extracted with ether showed two kinds of endogenous inhibitors：one that dissolved easily in water and the other that was easily soluble in a non-polar solvent.［Ch，2 fig. 4 tab. 11 ref.］
- silviculture,
- Pinus massoniana,
- line,
- endogenous inhibitor,
- cutting,
- purification,
- biological assay

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings

1. The Institute of Silviculture and Ecology，Guizhou University，Guiyang 550025，Guizhou，China;

2. Hunan Forestry Academy，Changsha 410004，Hunan，China

Corresponding author: DING Gui-jie

Publish Date: 2010-08-20

Key words:

Abstract: To improve the rooting rate of Pinus massoniana，endogenous inhibitors from cuttings of five P. massoniana lines were purified and separated using extraction and high pressure liquid chromatography （HPLC）. Biological assays along with analysis of variance （ANOVA） with 4 treatments of aether extracts and 3 replications with comparisons to Brassica chinensis seeds were employed. Results revealed seven components of endogenous inhibitors in each P. massoniana cutting belonging to flavones，phenols，and abscisic acid（ABA）. This indicated that their expression was controlled by genes with significant differences among lines（P = 0.032 9）. By comparison，an ANOVA for the influence of endogenous inhibitors on growth of B. chinensis roots was highly significant （P = 0.000 1）；whereas compared to the same component of endogenous inhibitors in the root growth of B. chinensis，inhibition with the five P. massoniana lines were not significantly different. Also，except for MI6（No. 6 of HLPC educts），correlation analyses between the relative content of endogenous inhibitors and the rooting rate of B. chinensis were negative. Biological assays for extractions and their residuals extracted with ether showed two kinds of endogenous inhibitors：one that dissolved easily in water and the other that was easily soluble in a non-polar solvent.［Ch，2 fig. 4 tab. 11 ref.］

Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings

doi: 10.11833/j.issn.2095-0756.2010.04.005

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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Proportional views

Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings

doi: 10.11833/j.issn.2095-0756.2010.04.005

1. The Institute of Silviculture and Ecology，Guizhou University，Guiyang 550025，Guizhou，China;

2. Hunan Forestry Academy，Changsha 410004，Hunan，China

Corresponding author: DING Gui-jie

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Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings

doi: 10.11833/j.issn.2095-0756.2010.04.005

Abstract

Relative Article

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通讯作者: 陈斌, bchen63@163.com

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Separation，purification，and identification of endogenous inhibitory substances in Pinus massoniana cuttings

doi: 10.11833/j.issn.2095-0756.2010.04.005

1. The Institute of Silviculture and Ecology，Guizhou University，Guiyang 550025，Guizhou，China; 2. Hunan Forestry Academy，Changsha 410004，Hunan，China

Corresponding author: DING Gui-jie

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1. The Institute of Silviculture and Ecology，Guizhou University，Guiyang 550025，Guizhou，China;

2. Hunan Forestry Academy，Changsha 410004，Hunan，China