进口黄檀属木材DNA提取与分子鉴定方法初步研究

伏建国; 刘金良; 杨晓军; 安榆林; 骆嘉言

doi:10.11833/j.issn.2095-0756.2013.04.025

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

进口黄檀属木材DNA提取与分子鉴定方法初步研究

伏建国 , 刘金良 , 杨晓军 , 安榆林 , 骆嘉言

文章导航 > 浙江农林大学学报 > 2013 > 30(4): 627-632

伏建国, 刘金良, 杨晓军, 安榆林, 骆嘉言. 进口黄檀属木材DNA提取与分子鉴定方法初步研究[J]. 浙江农林大学学报, 2013, 30(4): 627-632. doi: 10.11833/j.issn.2095-0756.2013.04.025

引用本文:

FU Jianguo, LIU Jinliang, YANG Xiaojun, AN Yulin, LUO Jiayan. DNA extraction and molecular identification of imported Dalbergia wood[J]. Journal of Zhejiang A&F University, 2013, 30(4): 627-632. doi: 10.11833/j.issn.2095-0756.2013.04.025

Citation:

FU Jianguo, LIU Jinliang, YANG Xiaojun, AN Yulin, LUO Jiayan. DNA extraction and molecular identification of imported Dalbergia wood[J]. Journal of Zhejiang A&F University, 2013, 30(4): 627-632. doi: 10.11833/j.issn.2095-0756.2013.04.025

进口黄檀属木材DNA提取与分子鉴定方法初步研究

doi: 10.11833/j.issn.2095-0756.2013.04.025

1.
江苏出入境检验检疫局，江苏南京 210001;
2.
南京林业大学木材工业学院，江苏南京 210037

详细信息

通信作者: 安榆林

DNA extraction and molecular identification of imported Dalbergia wood

1.
Jiangsu Entry-Exit Inspection and Quarantine Bureau，The People’s Republic of China，Nanjing 210001，Jiangsu，China;
2.
College of Wood Science and Technology，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

More Information

Corresponding author: AN Yulin

摘要: 由于黄檀属Dalbergia种类较多，很多种类通过形态学方法难以区分，给中国进口木材检验鉴定工作带来了很大困难。为了研究黄檀属木材的分子鉴定方法，选取进口木材中常见的7种黄檀属木材，通过比较不同的木材DNA提取和纯化方法，摸索出了适合于黄檀属木材的十六烷基三甲基溴化铵（CTAB）十二烷基硫酸钠（SDS）磁珠相结合的DNA提取和纯化体系，利用巢式聚合酶链式反应（PCR）扩增出433 bp的matK基因片段，共发现12个碱基位点差异，可以将7种进口黄檀属木材及我国的降香黄檀Dalbergia odorifera逐一区分开，为黄檀属木材分子识别鉴定研究工作奠定了良好的基础。图2表1参23
- 林业工程 /
- 黄檀属木材 /
- DNA提取 /
- 纯化 /
- 巢式PCR /
- matK基因 /
- 鉴定
Abstract: Establishing a reliable and cost effective method to identify and distinguish wood is important in preventing the illegal timber trade. To overcome the difficulty of distinguishing Dalbergia，which consists of many valuable species，by morphological methods，molecular genetic tools to control species identity were studied. Seven species of Dalbergia wood，D. melanaoxylon，D. oliveri，D. retusa，D. louvelii，D. greveana，D. cochinchinensis，D. cultrate，imported from different countries and one domestic species D. odorifera were selected for testing. Next，an array of different wood DNA extraction and purification methods，including DNeasy Plant Mini Kit，hexadecyltrimethylammonium bromide（CTAB），sodium dodecyl sulfate （SDS） and Magnetic Beads based method，were tried；For the amplification of DNA from wood samples, two PCR reactions were successively performed（nested PCR）and allowed the amplication of one coding region of chloroplast DNA （cpDNA）. Results showed that a CTAB-SDS-Magnetic Beads based method was available for Dalbergia wood DNA extraction. Then，433 bp matK gene fragments were amplified and sequenced from the seven species，of which a total of 12 single nucleotide polymorphisms were found. These polymorphic sites were able to distinguish the eight Dalbergia species. Thus，establishment of this method for DNA isolation and polymerase chain reaction （PCR） amplification makes Dalbergia wood amenable to DNA-based identification.［Ch，2 fig. 1 tab. 23 ref.］
- forest engineering /
- Dalbergia timber /
- DNA extraction /
- purification /
- nested-polymerase chain reaction （PCR） /
- matK /
- identification

[1]	骆静怡, 傅威锐, 潘程远. 木腐真菌的鉴定及对不同木材的腐朽能力 . 浙江农林大学学报, 2015, 32(1): 1-10. doi: 10.11833/j.issn.2095-0756.2015.01.001
[2]	周生财, 黄华宏, 童再康, 吴小林. 4种楠木AFLP反应体系优化建立 . 浙江农林大学学报, 2013, 30(5): 789-796. doi: 10.11833/j.issn.2095-0756.2013.05.024
[3]	沈红霞, 韩秀杰, 赵凡凡, 张保新, 余风艳, 王晓杜. 猪日本乙型脑炎病毒NS1基因的表达和抗体制备 . 浙江农林大学学报, 2013, 30(3): 396-400. doi: 10.11833/j.issn.2095-0756.2013.03.015
[4]	伏建国, 刘金良, 杨晓军, 安榆林, 骆嘉言. 分子生物学技术应用于木材识别的研究进展 . 浙江农林大学学报, 2013, 30(3): 438-443. doi: 10.11833/j.issn.2095-0756.2013.03.022
[5]	符韵林, 邱炳发, 韦鹏练, 廖克波, 刘晓玲, 袁振双. 观光木木材干燥特性研究 . 浙江农林大学学报, 2011, 28(5): 767-770. doi: 10.11833/j.issn.2095-0756.2011.05.013
[6]	方益明, 郑红平, 冯海林. 基于傅里叶变换和独立成分分析的木材显微图像特征提取与识别 . 浙江农林大学学报, 2010, 27(6): 826-830. doi: 10.11833/j.issn.2095-0756.2010.06.004
[7]	李永进, 丁贵杰. 不同家系马尾松插穗内源生根抑制物的分离、纯化及鉴定 . 浙江农林大学学报, 2010, 27(4): 507-512. doi: 10.11833/j.issn.2095-0756.2010.04.005
[8]	汪杭军, 张广群, 祁亨年, 李文珠. 木材识别方法研究综述 . 浙江农林大学学报, 2009, 26(6): 896-902.
[9]	李河, 宋光桃, 何末军, 郝艳, 周国英. 巢式PCR检测油茶根腐病菌的研究 . 浙江农林大学学报, 2009, 26(6): 849-853.
[10]	何沙娥, 张智俊. 适用于竹林土壤PCR-DGGE分析用的微生物总DNA提取及纯化方法 . 浙江农林大学学报, 2009, 26(2): 164-168.
[11]	杨燕, 邱坚. SEM-EDXA法测定分析柠檬桉木材中的晶体 . 浙江农林大学学报, 2008, 25(2): 143-147.
[12]	尹建新, 楼雄伟, 黄美丽. 灰度直方图在木材表面缺陷检测中的应用 . 浙江农林大学学报, 2008, 25(3): 272-276.
[13]	眭亚萍, 孙芳利, 杨中平, 王珊珊, 王丽. 木材防白蚁药剂研究概况及展望 . 浙江农林大学学报, 2008, 25(2): 250-254.
[14]	郭金剑, 池伟, 赵鑫珠, 王丽, 曾燕如, 张秋月. 山核桃AFLP实验技术体系的建立 . 浙江农林大学学报, 2008, 25(4): 532-537.
[15]	梁宏温, 黄恒川, 黄承标, 黄海仲, 梁欣, 蒙跃环. 不同树龄秃杉与杉木人工林木材物理力学性质的比较 . 浙江农林大学学报, 2008, 25(2): 137-142.
[16]	顾翠花, 王守先, 张启翔. 我国紫薇属植物AFLP分子标记体系的优化 . 浙江农林大学学报, 2008, 25(3): 298-303.
[17]	曾松伟, 刘敬彪, 周巧娣, 夏霞. 基于MSP 430 的木材干燥窑测控系统 . 浙江农林大学学报, 2006, 23(6): 673-677.
[18]	刘志军, 李延军, 张璧光, 刘智. 马尾松木材微波干燥特性的研究 . 浙江农林大学学报, 2006, 23(5): 482-485.
[19]	白红霞, 袁秀英. 内蒙古地区杨树内生真菌多样性调查 . 浙江农林大学学报, 2006, 23(6): 629-635.
[20]	程晓建, 杨萍, 林伯年, 郑炳松. 应用RAPD 技术探讨红叶李、李和杏的亲缘关系 . 浙江农林大学学报, 2004, 21(1): 40-43.

链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2013.04.025

https://zlxb.zafu.edu.cn/article/zjnldxxb/2013/4/627

点击查看大图

计量

文章访问数: 3788
HTML全文浏览量: 278
PDF下载量: 1045
被引次数: 0

进口黄檀属木材DNA提取与分子鉴定方法初步研究

doi: 10.11833/j.issn.2095-0756.2013.04.025

1. 江苏出入境检验检疫局，江苏南京 210001;

2. 南京林业大学木材工业学院，江苏南京 210037

通信作者: 安榆林

收稿日期: 2012-04-27
修回日期: 2012-06-13
刊出日期: 2013-08-20

关键词:

摘要: 由于黄檀属Dalbergia种类较多，很多种类通过形态学方法难以区分，给中国进口木材检验鉴定工作带来了很大困难。为了研究黄檀属木材的分子鉴定方法，选取进口木材中常见的7种黄檀属木材，通过比较不同的木材DNA提取和纯化方法，摸索出了适合于黄檀属木材的十六烷基三甲基溴化铵（CTAB）十二烷基硫酸钠（SDS）磁珠相结合的DNA提取和纯化体系，利用巢式聚合酶链式反应（PCR）扩增出433 bp的matK基因片段，共发现12个碱基位点差异，可以将7种进口黄檀属木材及我国的降香黄檀Dalbergia odorifera逐一区分开，为黄檀属木材分子识别鉴定研究工作奠定了良好的基础。图2表1参23

English Abstract

DNA extraction and molecular identification of imported Dalbergia wood

1. Jiangsu Entry-Exit Inspection and Quarantine Bureau，The People’s Republic of China，Nanjing 210001，Jiangsu，China;

2. College of Wood Science and Technology，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

Corresponding author: AN Yulin

Received Date: 2012-04-27
Rev Recd Date: 2012-06-13
Publish Date: 2013-08-20

Keywords:

forest engineering /
Dalbergia timber /
DNA extraction /
purification /
nested-polymerase chain reaction （PCR） /
matK /
identification

Abstract: Establishing a reliable and cost effective method to identify and distinguish wood is important in preventing the illegal timber trade. To overcome the difficulty of distinguishing Dalbergia，which consists of many valuable species，by morphological methods，molecular genetic tools to control species identity were studied. Seven species of Dalbergia wood，D. melanaoxylon，D. oliveri，D. retusa，D. louvelii，D. greveana，D. cochinchinensis，D. cultrate，imported from different countries and one domestic species D. odorifera were selected for testing. Next，an array of different wood DNA extraction and purification methods，including DNeasy Plant Mini Kit，hexadecyltrimethylammonium bromide（CTAB），sodium dodecyl sulfate （SDS） and Magnetic Beads based method，were tried；For the amplification of DNA from wood samples, two PCR reactions were successively performed（nested PCR）and allowed the amplication of one coding region of chloroplast DNA （cpDNA）. Results showed that a CTAB-SDS-Magnetic Beads based method was available for Dalbergia wood DNA extraction. Then，433 bp matK gene fragments were amplified and sequenced from the seven species，of which a total of 12 single nucleotide polymorphisms were found. These polymorphic sites were able to distinguish the eight Dalbergia species. Thus，establishment of this method for DNA isolation and polymerase chain reaction （PCR） amplification makes Dalbergia wood amenable to DNA-based identification.［Ch，2 fig. 1 tab. 23 ref.］

引用本文: