Vitrification of chondrocyte from <em>Ovis aries</em>

HUANG Pei-long; ZHANG Shao-zhi; YU Xiao-yi; HU Jun-xiang

doi:10.11833/j.issn.2095-0756.2011.02.016

Volume 28 Issue 2

Apr. 2011

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HUANG Pei-long, ZHANG Shao-zhi, YU Xiao-yi, HU Jun-xiang. Vitrification of chondrocyte from Ovis aries[J]. Journal of Zhejiang A&F University, 2011, 28(2): 269-274. doi: 10.11833/j.issn.2095-0756.2011.02.016

Citation:

HUANG Pei-long, ZHANG Shao-zhi, YU Xiao-yi, HU Jun-xiang. Vitrification of chondrocyte from Ovis aries[J]. Journal of Zhejiang A&F University, 2011, 28(2): 269-274. doi: 10.11833/j.issn.2095-0756.2011.02.016

Vitrification of chondrocyte from Ovis aries

doi: 10.11833/j.issn.2095-0756.2011.02.016

1.
School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;
2.
Institute of Refrigeration and Cryogenics，Zhejiang University，Hangzhou 310027，Zhejiang，China

More Information

Corresponding author: HU Jun-xiang
Publish Date: 2011-04-20

Abstract

The effects of vitrification on survival rate and succinate dehydrogenase（SDH） activity of ovine chondrocytes were studied. Results showed that the SDH activity decreased remarkably within 20 d after vitrification, whereas the survival rate decreased slowly. VSb selected according to the trend of survival rates and vitality of SDH. After being cryopreserved for 5，10，15，20 and 30 days，the survival rates were 85.47% 1.78%，80.73% 1.81%，78.62% 2.06%，76.35% 2.58% and 73.83% 1.49%，and the vitality of SDH showed 0.49 0.064，0.444 0.073，0.394 0.039，0.354 0.082 and 0.339 0.053. This indicated that cryopreservation of ovine chondrocytes by vitrification had some practical value. [Ch, 4 fig. 2 tab. 17 ref.]
- zoology,
- vitrification,
- chondrocytes,
- survival rate,
- succinate dehydrogenase（SDH）,
- Ovis aries

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Vitrification of chondrocyte from Ovis aries

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Institute of Refrigeration and Cryogenics，Zhejiang University，Hangzhou 310027，Zhejiang，China

Corresponding author: HU Jun-xiang

Publish Date: 2011-04-20

Key words:

Abstract: The effects of vitrification on survival rate and succinate dehydrogenase（SDH） activity of ovine chondrocytes were studied. Results showed that the SDH activity decreased remarkably within 20 d after vitrification, whereas the survival rate decreased slowly. VSb selected according to the trend of survival rates and vitality of SDH. After being cryopreserved for 5，10，15，20 and 30 days，the survival rates were 85.47% 1.78%，80.73% 1.81%，78.62% 2.06%，76.35% 2.58% and 73.83% 1.49%，and the vitality of SDH showed 0.49 0.064，0.444 0.073，0.394 0.039，0.354 0.082 and 0.339 0.053. This indicated that cryopreservation of ovine chondrocytes by vitrification had some practical value. [Ch, 4 fig. 2 tab. 17 ref.]

Vitrification of chondrocyte from Ovis aries

doi: 10.11833/j.issn.2095-0756.2011.02.016

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Vitrification of chondrocyte from Ovis aries

doi: 10.11833/j.issn.2095-0756.2011.02.016

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Institute of Refrigeration and Cryogenics，Zhejiang University，Hangzhou 310027，Zhejiang，China

Corresponding author: HU Jun-xiang

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Vitrification of chondrocyte from Ovis aries

doi: 10.11833/j.issn.2095-0756.2011.02.016

Abstract

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通讯作者: 陈斌, bchen63@163.com

Related

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Vitrification of chondrocyte from Ovis aries

doi: 10.11833/j.issn.2095-0756.2011.02.016

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China; 2. Institute of Refrigeration and Cryogenics，Zhejiang University，Hangzhou 310027，Zhejiang，China

Corresponding author: HU Jun-xiang

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1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Institute of Refrigeration and Cryogenics，Zhejiang University，Hangzhou 310027，Zhejiang，China