Soil microbial biomass and biotic diversity with Bt insecticide

CHEN Mengyang; WEI Jian; GE Gaobo; SUN Ming; FENG Xiuzhi; WU Keren; LI Yongchun; XU Qiufang

doi:10.11833/j.issn.2095-0756.2013.05.005

Volume 30 Issue 5

Sep. 2013

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CHEN Mengyang, WEI Jian, GE Gaobo, SUN Ming, FENG Xiuzhi, WU Keren, LI Yongchun, XU Qiufang. Soil microbial biomass and biotic diversity with Bt insecticide[J]. Journal of Zhejiang A&F University, 2013, 30(5): 662-668. doi: 10.11833/j.issn.2095-0756.2013.05.005

Citation:

CHEN Mengyang, WEI Jian, GE Gaobo, SUN Ming, FENG Xiuzhi, WU Keren, LI Yongchun, XU Qiufang. Soil microbial biomass and biotic diversity with Bt insecticide[J]. Journal of Zhejiang A&F University, 2013, 30(5): 662-668. doi: 10.11833/j.issn.2095-0756.2013.05.005

Soil microbial biomass and biotic diversity with Bt insecticide

doi: 10.11833/j.issn.2095-0756.2013.05.005

1.
School of Environmental and Resources Science，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;
2.
Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: LI Yongchun
Received Date: 2012-12-21
Rev Recd Date: 2013-02-27
Publish Date: 2013-10-20

Abstract

Environmental safety assessment of soil microbial is essential for development and application of Bt insecticide. To analyze the effects of Bt insecticide on soil microbial in vegetable and pine forest soils，a chloroform fumigation-extraction method and a denaturing gradient gel electrophoresis（DGGE）were used to assess microbial biomass and the bacterial community after spraying insecticide Bt with three concentration gradients. Results indicated that, after spraying Bt insecticide at 375，1 315，and 2 255 ghm－2，soil microbial biomass carbon（MBC）for vegetable soil, significantly decreased, increased and then decreased（P＜ 0.05）， respectively；whereas, for a pine forest soil, when spraying at concentration gradients of 1 315，2 255 ghm－2，MBC significantly increased（P＜0.05）. Compared with the samples without spraying Bt，MBC for both vegetable soils and pine forest soils reached a maximum at 1 315 ghm－2 of Bt. Also, compared to pine forest soils，DGGE patterns for vegetable soils revealed a higher richness in the bacterial community. After spraying Bt insecticide on vegetable soils，soil bacterial community diversity indices significantly increased（P ＜0.05），but for pine forest soils they significantly decreased（P＜0.05）or were unchanged with different concentration gradient. In this study，soil types were a key determinant of soil bacterial community structure, and spraying Bt insecticide did not lead to new community structures.［Ch，3 fig. 3 tab. 20 ref.］
- soil science,
- Bt insecticide,
- soil microbial biomass,
- bacterial community structure,
- diversity indices

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Soil microbial biomass and biotic diversity with Bt insecticide

1. School of Environmental and Resources Science，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: LI Yongchun

Received Date: 2012-12-21

Rev Recd Date: 2013-02-27

Publish Date: 2013-10-20

Key words:

Abstract: Environmental safety assessment of soil microbial is essential for development and application of Bt insecticide. To analyze the effects of Bt insecticide on soil microbial in vegetable and pine forest soils，a chloroform fumigation-extraction method and a denaturing gradient gel electrophoresis（DGGE）were used to assess microbial biomass and the bacterial community after spraying insecticide Bt with three concentration gradients. Results indicated that, after spraying Bt insecticide at 375，1 315，and 2 255 ghm－2，soil microbial biomass carbon（MBC）for vegetable soil, significantly decreased, increased and then decreased（P＜ 0.05）， respectively；whereas, for a pine forest soil, when spraying at concentration gradients of 1 315，2 255 ghm－2，MBC significantly increased（P＜0.05）. Compared with the samples without spraying Bt，MBC for both vegetable soils and pine forest soils reached a maximum at 1 315 ghm－2 of Bt. Also, compared to pine forest soils，DGGE patterns for vegetable soils revealed a higher richness in the bacterial community. After spraying Bt insecticide on vegetable soils，soil bacterial community diversity indices significantly increased（P ＜0.05），but for pine forest soils they significantly decreased（P＜0.05）or were unchanged with different concentration gradient. In this study，soil types were a key determinant of soil bacterial community structure, and spraying Bt insecticide did not lead to new community structures.［Ch，3 fig. 3 tab. 20 ref.］

Soil microbial biomass and biotic diversity with Bt insecticide

doi: 10.11833/j.issn.2095-0756.2013.05.005

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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