Pollen vitality and stigma receptivity of <em>Cymbidium hybridum</em> and Chinese orchid

CHU Yi; FAN Yirong; ZHANG Shaoyi; SUN Yufen; NING Huijuan

doi:10.11833/j.issn.2095-0756.2013.06.022

Volume 30 Issue 6

Nov. 2013

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CHU Yi, FAN Yirong, ZHANG Shaoyi, SUN Yufen, NING Huijuan. Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid[J]. Journal of Zhejiang A&F University, 2013, 30(6): 950-954. doi: 10.11833/j.issn.2095-0756.2013.06.022

Citation:

CHU Yi, FAN Yirong, ZHANG Shaoyi, SUN Yufen, NING Huijuan. Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid[J]. Journal of Zhejiang A&F University, 2013, 30(6): 950-954. doi: 10.11833/j.issn.2095-0756.2013.06.022

Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid

doi: 10.11833/j.issn.2095-0756.2013.06.022

1.
School of Landscape Architecture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: NING Huijuan
Received Date: 2012-11-12
Rev Recd Date: 2013-01-18
Publish Date: 2013-12-20

Abstract

To ensure effective cross-breeding work，high vitality of pollen and stigma receptivity are required. 2,3,5-triphenyltetrazolium chloride（TTC），iodine-potassium iodide（I2-KI），and Carmine staining methods were used to determine pollen viability for Cymbidium hybridum and Chinese orchid；benzidine-H2O2 was utilized to determine stigma receptivity and to observe stigma mucus secretion. Results indicated that the I2-KI and Carmine methods were not suited to test pollen vitality while the TTC method verified to be best. Throughout flowering，the stigmas of Cymbidium hybridum and Chinese orchid were receptive. The stigma of Cymbidium hybridum reached its highest receptivity from 20 d to 40 d，after flowering，stigma receptivity gradually weakened after 45 d. For the Chinese orchid from about 15 d to 25 d，receptivity strengthened，but at 30 d，stigma receptivity gradually weakened and disappeared. Thus，the stigma receptivity process changed from weak to strong to weak.［Ch，2 fig. 2 tab. 19 ref.］
- horticulture,
- Cymbidium hybridum,
- Chinese orchid,
- pollen vitality,
- stigma receptivity

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Proportional views

Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid

1. School of Landscape Architecture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: NING Huijuan

Received Date: 2012-11-12

Rev Recd Date: 2013-01-18

Publish Date: 2013-12-20

Key words:

Abstract: To ensure effective cross-breeding work，high vitality of pollen and stigma receptivity are required. 2,3,5-triphenyltetrazolium chloride（TTC），iodine-potassium iodide（I2-KI），and Carmine staining methods were used to determine pollen viability for Cymbidium hybridum and Chinese orchid；benzidine-H2O2 was utilized to determine stigma receptivity and to observe stigma mucus secretion. Results indicated that the I2-KI and Carmine methods were not suited to test pollen vitality while the TTC method verified to be best. Throughout flowering，the stigmas of Cymbidium hybridum and Chinese orchid were receptive. The stigma of Cymbidium hybridum reached its highest receptivity from 20 d to 40 d，after flowering，stigma receptivity gradually weakened after 45 d. For the Chinese orchid from about 15 d to 25 d，receptivity strengthened，but at 30 d，stigma receptivity gradually weakened and disappeared. Thus，the stigma receptivity process changed from weak to strong to weak.［Ch，2 fig. 2 tab. 19 ref.］

Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid

doi: 10.11833/j.issn.2095-0756.2013.06.022

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通讯作者: 陈斌, bchen63@163.com

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Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid

doi: 10.11833/j.issn.2095-0756.2013.06.022

1. School of Landscape Architecture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: NING Huijuan

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Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid

doi: 10.11833/j.issn.2095-0756.2013.06.022

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通讯作者: 陈斌, bchen63@163.com

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Pollen vitality and stigma receptivity of Cymbidium hybridum and Chinese orchid

doi: 10.11833/j.issn.2095-0756.2013.06.022

1. School of Landscape Architecture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: NING Huijuan

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