Cloning and analysis of a MADS-like gene in<em> Carya cathayensis</em>

HOU Chuanming; ZHENG Yawen; WANG Zhengjia; XU Yingwu

doi:10.11833/j.issn.2095-0756.2015.01.005

Volume 32 Issue 1

Jan. 2015

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HOU Chuanming, ZHENG Yawen, WANG Zhengjia, XU Yingwu. Cloning and analysis of a MADS-like gene in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(1): 33-39. doi: 10.11833/j.issn.2095-0756.2015.01.005

Citation:

HOU Chuanming, ZHENG Yawen, WANG Zhengjia, XU Yingwu. Cloning and analysis of a MADS-like gene in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2015, 32(1): 33-39. doi: 10.11833/j.issn.2095-0756.2015.01.005

Cloning and analysis of a MADS-like gene in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.01.005

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Received Date: 2014-03-10
Rev Recd Date: 2014-04-20
Publish Date: 2015-02-20

Abstract

MADS-box family genes play key roles in all phases of floral development. Based on a partial fragment acquired from 454 sequencing of Carya cathayensis （hickory） floral buds, a full-length of a MADS-like gene was obtained by rapid amplification of cDNA ends （RACE）. Afterwards, a quantitative real-time （qRT） polymerase chain reaction （PCR） analysis, an amino acid sequence alignment, and a yeast one-hybrid assay were performed. Results of the sequence analysis revealed the open reading frame （ORF） of the CcMADS-like gene was 609 bp in length and encoded a protein of 202 aa. The protein presented typical characteristic of MADS-box family genes and contained MADS domain, I, K, and C, with the MADS domain was considerably conserved. The amino acid sequence alignment showed that the putative protein CcMADS-like gene had an identity to homologies of Arabidopsis thaliana （48%）, Antirrhinum majus （65%）, Populus deltoides （69%）, and Populus trichocarpa （71%）. The qRT-PCR analysis revealed that the expression level of CcMADS-like gene in flower buds was much higher than in other tissues. The yeast one-hybrid showed that the CcMADS-like gene had an activation activity as a transcription factor. Overall, the qRT-PCR analysis suggested that CcMADS-like gene possibly participated in floral development of hickory. ［Ch, 5 fig. 1 tab. 25 ref.］
- cash forestry,
- floral development,
- Carya cathayensis,
- MADS-box,
- gene clone,
- qRT-PCR,
- yeast one-hybrid

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and analysis of a MADS-like gene in Carya cathayensis

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Received Date: 2014-03-10

Rev Recd Date: 2014-04-20

Publish Date: 2015-02-20

Key words:

Abstract: MADS-box family genes play key roles in all phases of floral development. Based on a partial fragment acquired from 454 sequencing of Carya cathayensis （hickory） floral buds, a full-length of a MADS-like gene was obtained by rapid amplification of cDNA ends （RACE）. Afterwards, a quantitative real-time （qRT） polymerase chain reaction （PCR） analysis, an amino acid sequence alignment, and a yeast one-hybrid assay were performed. Results of the sequence analysis revealed the open reading frame （ORF） of the CcMADS-like gene was 609 bp in length and encoded a protein of 202 aa. The protein presented typical characteristic of MADS-box family genes and contained MADS domain, I, K, and C, with the MADS domain was considerably conserved. The amino acid sequence alignment showed that the putative protein CcMADS-like gene had an identity to homologies of Arabidopsis thaliana （48%）, Antirrhinum majus （65%）, Populus deltoides （69%）, and Populus trichocarpa （71%）. The qRT-PCR analysis revealed that the expression level of CcMADS-like gene in flower buds was much higher than in other tissues. The yeast one-hybrid showed that the CcMADS-like gene had an activation activity as a transcription factor. Overall, the qRT-PCR analysis suggested that CcMADS-like gene possibly participated in floral development of hickory. ［Ch, 5 fig. 1 tab. 25 ref.］

Cloning and analysis of a MADS-like gene in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.01.005

Abstract

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Cloning and analysis of a MADS-like gene in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.01.005

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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Cloning and analysis of a MADS-like gene in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.01.005

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Cloning and analysis of a MADS-like gene in Carya cathayensis

doi: 10.11833/j.issn.2095-0756.2015.01.005

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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