Pollen-wall protein extraction and characteristics of <em>Catalpa bungei</em>

WANG Gaiping; XU Tao; FAN Lili; PENG Fangren; Lv Xin; CHEN Linyue

doi:10.11833/j.issn.2095-0756.2016.01.009

Volume 33 Issue 1

Jan. 2016

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WANG Gaiping, XU Tao, FAN Lili, PENG Fangren, Lv Xin, CHEN Linyue. Pollen-wall protein extraction and characteristics of Catalpa bungei[J]. Journal of Zhejiang A&F University, 2016, 33(1): 65-70. doi: 10.11833/j.issn.2095-0756.2016.01.009

Citation:

WANG Gaiping, XU Tao, FAN Lili, PENG Fangren, Lv Xin, CHEN Linyue. Pollen-wall protein extraction and characteristics of Catalpa bungei[J]. Journal of Zhejiang A&F University, 2016, 33(1): 65-70. doi: 10.11833/j.issn.2095-0756.2016.01.009

Pollen-wall protein extraction and characteristics of Catalpa bungei

doi: 10.11833/j.issn.2095-0756.2016.01.009

1.
College of Forestry, Nanjing Forstry University, Nanjing 210037, Jiangsu, China

Funds:

十二五国家科技支撑计划子项目（2012BAD21B03）;江苏高校优势学科建设工程资助项目（PAPD）

Received Date: 2015-03-02
Rev Recd Date: 2015-06-02
Publish Date: 2016-02-20

Abstract

The research was conducted to further reveal the self-incompatibility that exists extensively Catalpa bungei and to evaluate the best way to extract pollen-wall protein of Catalpa, including the Catalpa bungei plants CB-1 from Yuntaishan National Forest Park of Lianyungang and CB-2 from Laoshan Forest of Nanjing and Catalpa fargesii f. duclouxii （CF） using incorporation and ultrasonication. Also, the content and component of pollen-wall protein from different varieties was compared by the Coomassie Blue Staining Method and SDS （sodium dodecyl sulfate）-polyacrylamide gel electrophoresis （PAGE）. Results showed that the relative optimization method to extract pollen-wall protein of C. bungei was ultrasonication with the optimal parameters being: ultrasonication at 400 W power for 3 s every 6 s for 3 rounds taking 120 duplications at an interval of 5 min. The optimal pH value to extract pollen-wall protein was pH 7.8（F＞F0.05）. For pollen-wall protein content, CF was higher（F＞F0.01） than both CB-1 and CB-2. From the pollen of these three catalpas, 26 proteins were found. Molecular weight of the specific proteins for CB-1 were 53.8 kDa, 35.0 kDa, 23.4 kDa, and 26.5 kDa; for CB-2 they were 53.8 kDa, 38.5 kDa, and 23.4 kDa; and for CF they were 8.5 kDa and 26.5 kDa.［Ch, 6 fig. 1 tab. 18 ref.］
- botany,
- Catalpa bungei,
- ultrasonication,
- protein,
- SDS-PAGE

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Pollen-wall protein extraction and characteristics of Catalpa bungei

1. College of Forestry, Nanjing Forstry University, Nanjing 210037, Jiangsu, China

Funds:

十二五国家科技支撑计划子项目（2012BAD21B03）;江苏高校优势学科建设工程资助项目（PAPD）

Received Date: 2015-03-02

Rev Recd Date: 2015-06-02

Publish Date: 2016-02-20

Key words:

Abstract: The research was conducted to further reveal the self-incompatibility that exists extensively Catalpa bungei and to evaluate the best way to extract pollen-wall protein of Catalpa, including the Catalpa bungei plants CB-1 from Yuntaishan National Forest Park of Lianyungang and CB-2 from Laoshan Forest of Nanjing and Catalpa fargesii f. duclouxii （CF） using incorporation and ultrasonication. Also, the content and component of pollen-wall protein from different varieties was compared by the Coomassie Blue Staining Method and SDS （sodium dodecyl sulfate）-polyacrylamide gel electrophoresis （PAGE）. Results showed that the relative optimization method to extract pollen-wall protein of C. bungei was ultrasonication with the optimal parameters being: ultrasonication at 400 W power for 3 s every 6 s for 3 rounds taking 120 duplications at an interval of 5 min. The optimal pH value to extract pollen-wall protein was pH 7.8（F＞F0.05）. For pollen-wall protein content, CF was higher（F＞F0.01） than both CB-1 and CB-2. From the pollen of these three catalpas, 26 proteins were found. Molecular weight of the specific proteins for CB-1 were 53.8 kDa, 35.0 kDa, 23.4 kDa, and 26.5 kDa; for CB-2 they were 53.8 kDa, 38.5 kDa, and 23.4 kDa; and for CF they were 8.5 kDa and 26.5 kDa.［Ch, 6 fig. 1 tab. 18 ref.］

Pollen-wall protein extraction and characteristics of Catalpa bungei

doi: 10.11833/j.issn.2095-0756.2016.01.009

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Pollen-wall protein extraction and characteristics of Catalpa bungei

doi: 10.11833/j.issn.2095-0756.2016.01.009

1. College of Forestry, Nanjing Forstry University, Nanjing 210037, Jiangsu, China

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Pollen-wall protein extraction and characteristics of Catalpa bungei

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Pollen-wall protein extraction and characteristics of Catalpa bungei

doi: 10.11833/j.issn.2095-0756.2016.01.009

1. College of Forestry, Nanjing Forstry University, Nanjing 210037, Jiangsu, China

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