Factors influencing vitreous shoots of fine Melia azedarach clones in vitro

Key words: 
• silviculture /
• Melia azedarach /
• tissue culture /
• vitrification

Abstract: With the nodal segments and apical buds from fine mother stocks of Melia azedarach clones as explants , the effects of such factors as BA and NAA concentration ratio , sugar and agar concentration , culture vessel and sealing materials on the vitreous shoots of Melia azedarach in vitro were studied .The results indicated that BA had an evident effect on the vitrification of shoots .When BA was at the concentrations between 0.5 -1.0 mgL-1 , vitreous shoots increased significantly with the increasing concentration of BA .The shoots in the culture media supplemented with 0.75 mgL-1BA +0.15 mgL-1 NAA could get a considerable multiplication coefficient (about 4.95), and also grew robustly .Mass fraction of sugar also had an evident effect on the vitrification of shoots .40 g L-1 sugar helped control the shoot vitrification , vitreous shoots only accounting for 18.33 %.Agar concentration had no evident effect .The containers or sealing materials with good air-permeability could significantly reduce the vitreous shoots and promote robust growth of the shoots .[ Ch , 3 tab .9 ref .]