Optimization of the reaction system for CAPS marker analysis in <em>Cryptomeria fortunei</em>

YANG Ping; ZHENG Qian-jian; ZHAO Ming-shui; TANG Ding-qin

Volume 23 Issue 1

Feb. 2017

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YANG Ping, ZHENG Qian-jian, ZHAO Ming-shui, TANG Ding-qin. Optimization of the reaction system for CAPS marker analysis in Cryptomeria fortunei[J]. Journal of Zhejiang A&F University, 2006, 23(1): 52-55.

Citation:

YANG Ping, ZHENG Qian-jian, ZHAO Ming-shui, TANG Ding-qin. Optimization of the reaction system for CAPS marker analysis in Cryptomeria fortunei[J]. Journal of Zhejiang A&F University, 2006, 23(1): 52-55.

Optimization of the reaction system for CAPS marker analysis in Cryptomeria fortunei

1.
Key Labratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;
2.
.Management Office , National Nature Reserve of Mount Tianmu , Lin’an 311300 , Zhejiang , China

Received Date: 2004-11-16
Rev Recd Date: 2005-09-29
Publish Date: 2006-02-20

Abstract

An optimized system for CAPS reaction in Cryptomeria fortunei is a precondition for analyzing genetic diversity of Cryptomeria fortunei .The authors optimized a reaction system for CAPS in Cryptomeria fortunei by analyzing different factors .The results showed that the best one was 20 L amplification reaction solution composed of 2.0 L 10 Buffer , 0.08 mmolL-1dNTPs , 200 mgL-1 template DNA , 1.5 mmolL-1 Mg2+ , 0.1 mol L-1primer and 41.675 nkat Taq DNA polymerase , which was amplified under the program of predenaturing at 94 ℃ for 5min , followed by 35 cycles of denaturing at 94 ℃for 40 s , annealing 58 ℃for 40 s , extension 72 ℃for 80 s , and a final extension 72 ℃ for 5 min .The restriction digestion system consisted of 10 L PCR products , 2.0 L 10 Buffer , 83.350 nkat restriction enzyme and 7.5 L ddH2O .[ Ch , 5 fig .1 tab .8 ref .]
- botany,
- Cryptomeria fortunei,
- CAPS,
- DNA marker,
- reaction system,
- optimization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Optimization of the reaction system for CAPS marker analysis in Cryptomeria fortunei

1. Key Labratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. .Management Office , National Nature Reserve of Mount Tianmu , Lin’an 311300 , Zhejiang , China

Received Date: 2004-11-16

Rev Recd Date: 2005-09-29

Publish Date: 2006-02-20

Key words:

Abstract: An optimized system for CAPS reaction in Cryptomeria fortunei is a precondition for analyzing genetic diversity of Cryptomeria fortunei .The authors optimized a reaction system for CAPS in Cryptomeria fortunei by analyzing different factors .The results showed that the best one was 20 L amplification reaction solution composed of 2.0 L 10 Buffer , 0.08 mmolL-1dNTPs , 200 mgL-1 template DNA , 1.5 mmolL-1 Mg2+ , 0.1 mol L-1primer and 41.675 nkat Taq DNA polymerase , which was amplified under the program of predenaturing at 94 ℃ for 5min , followed by 35 cycles of denaturing at 94 ℃for 40 s , annealing 58 ℃for 40 s , extension 72 ℃for 80 s , and a final extension 72 ℃ for 5 min .The restriction digestion system consisted of 10 L PCR products , 2.0 L 10 Buffer , 83.350 nkat restriction enzyme and 7.5 L ddH2O .[ Ch , 5 fig .1 tab .8 ref .]

Optimization of the reaction system for CAPS marker analysis in Cryptomeria fortunei

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Optimization of the reaction system for CAPS marker analysis in Cryptomeria fortunei

1. Key Labratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. .Management Office , National Nature Reserve of Mount Tianmu , Lin’an 311300 , Zhejiang , China

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Optimization of the reaction system for CAPS marker analysis in Cryptomeria fortunei

1. Key Labratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China; 2. .Management Office , National Nature Reserve of Mount Tianmu , Lin’an 311300 , Zhejiang , China

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1. Key Labratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. .Management Office , National Nature Reserve of Mount Tianmu , Lin’an 311300 , Zhejiang , China