Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis

CHENG Xiao-jian; YANG Ping; LIN Bo-nian; ZHENG Bing-song

Volume 21 Issue 1

Feb. 2017

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CHENG Xiao-jian, YANG Ping, LIN Bo-nian, ZHENG Bing-song. Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis[J]. Journal of Zhejiang A&F University, 2004, 21(1): 40-43.

Citation:

CHENG Xiao-jian, YANG Ping, LIN Bo-nian, ZHENG Bing-song. Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis[J]. Journal of Zhejiang A&F University, 2004, 21(1): 40-43.

Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis

1.
.School of Life Sciences, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;
2.
College of Agriculture and Biotechnology , Zhejiang University , Hangzhou 310029, Zhejiang , China

Funds:

浙江省科学技术厅重大项目资助(021102537)

Received Date: 2003-10-27
Rev Recd Date: 2003-12-29
Publish Date: 2004-03-20

Abstract

To provide evidence for conservation and identification of genetic relationship among myrobalan plum, plum and apricot cultivars and testify the traditional morphology-based taxonomy , genomic DNA's of 8 accessions are amplified by RAPD (random amplified polymorphic DNA)with 20 primers selected from 132 random primers .The result shows that they are all of polymorphism.264 fragments had been amplified , with 13.2 RAPD fragments amplified from each primer on the average .Clustering analysis shows that myrobalan plum , plum and apricot cultivars can be distinguished by RAPD ;there is some genetic relationship among them ;and the distance between varieties of each genus is different .The genetic distance reflects genetic relationship among tested materials .It has been proved that RAPD technique can be used for the classification and identification of genera .We can identify each cultivar of tested materials by DNA fingerprints and the specific bands .It shows that RAPD can be used for the identification of species or cultivars .[ Ch , 2 fig .2 tab .12 ref .]
- botany,
- myrobalan plum,
- plum,
- apricot,
- RAPD,
- genetic relationships,
- identification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis

1. .School of Life Sciences, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. College of Agriculture and Biotechnology , Zhejiang University , Hangzhou 310029, Zhejiang , China

Funds:

浙江省科学技术厅重大项目资助(021102537)

Received Date: 2003-10-27

Rev Recd Date: 2003-12-29

Publish Date: 2004-03-20

Key words:

Abstract: To provide evidence for conservation and identification of genetic relationship among myrobalan plum, plum and apricot cultivars and testify the traditional morphology-based taxonomy , genomic DNA's of 8 accessions are amplified by RAPD (random amplified polymorphic DNA)with 20 primers selected from 132 random primers .The result shows that they are all of polymorphism.264 fragments had been amplified , with 13.2 RAPD fragments amplified from each primer on the average .Clustering analysis shows that myrobalan plum , plum and apricot cultivars can be distinguished by RAPD ;there is some genetic relationship among them ;and the distance between varieties of each genus is different .The genetic distance reflects genetic relationship among tested materials .It has been proved that RAPD technique can be used for the classification and identification of genera .We can identify each cultivar of tested materials by DNA fingerprints and the specific bands .It shows that RAPD can be used for the identification of species or cultivars .[ Ch , 2 fig .2 tab .12 ref .]

Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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