Optimization of RAPD conditions of <em>Sargentodoxa cuneata</em>

JIN Ze-xin; LI Jun-min; ZHONG Zhang-cheng

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Volume 20 Issue 2

Jul. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2003 > 20(2): 141-145

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

Citation:

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

Optimization of RAPD conditions of Sargentodoxa cuneata

1.
Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;
2.
.Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

Funds:

国家自然科学基金资助项目(39870160)

Received Date: 2002-08-02
Rev Recd Date: 2003-01-13
Publish Date: 2003-06-20

Abstract

The optimized system used in RAPD reaction of Sargentodoxa cuneata must be established before analyzing the genetic diversity of Sargentodoxa cuneata .It is necessary to explore the reaction conditions .The genomic DNA of Sargentodoxa cuneata is extracted with improved SDS method and RAPD amplification is carried out .The effect of content of Mg , Dutp , DNA templates , primers and DNA polymerase on experimental results is tested and the optimal reaction system of RAPD for Sargentodoxa cuneata is determined as follows :1 Taq polymerase corresponding buffer (10 mmolL-1Tris HCl pH 9.0 , 50 mmolL-1KCl , 0.1 % Triton X-100 , 1.5 mmolL-1MgCl2), 25.01 10-9mols-1Taq DNA polymerase , 10 ng template DNA , 15 pmol primer , 2.00 gL-1BSA .0.15 mmolL-1dATP , dCTP , dGTP , dTTP for each in total 15 L reaction volume .[ Ch , 4 fig .2 tab .8 ref .]
- Sargentodoxa cuneata,
- RAPD,
- chemical composition,
- medicinal plant

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Optimization of RAPD conditions of Sargentodoxa cuneata

1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;

2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

Funds:

国家自然科学基金资助项目(39870160)

Received Date: 2002-08-02
Rev Recd Date: 2003-01-13
Publish Date: 2003-06-20

Key words:

Abstract: The optimized system used in RAPD reaction of Sargentodoxa cuneata must be established before analyzing the genetic diversity of Sargentodoxa cuneata .It is necessary to explore the reaction conditions .The genomic DNA of Sargentodoxa cuneata is extracted with improved SDS method and RAPD amplification is carried out .The effect of content of Mg , Dutp , DNA templates , primers and DNA polymerase on experimental results is tested and the optimal reaction system of RAPD for Sargentodoxa cuneata is determined as follows :1 Taq polymerase corresponding buffer (10 mmolL-1Tris HCl pH 9.0 , 50 mmolL-1KCl , 0.1 % Triton X-100 , 1.5 mmolL-1MgCl2), 25.01 10-9mols-1Taq DNA polymerase , 10 ng template DNA , 15 pmol primer , 2.00 gL-1BSA .0.15 mmolL-1dATP , dCTP , dGTP , dTTP for each in total 15 L reaction volume .[ Ch , 4 fig .2 tab .8 ref .]

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

Citation:

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

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Optimization of RAPD conditions of Sargentodoxa cuneata

1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;

2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

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Optimization of RAPD conditions of Sargentodoxa cuneata

1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China; 2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

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1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;

2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China