Optimization of RAPD conditions of <em>Sargentodoxa cuneata</em>

JIN Ze-xin; LI Jun-min; ZHONG Zhang-cheng

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 20 Issue 2

Jul. 2017

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2003 > 20(2): 141-145

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

Citation:

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

Optimization of RAPD conditions of Sargentodoxa cuneata

1.
Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;
2.
.Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

Funds:

国家自然科学基金资助项目(39870160)

Received Date: 2002-08-02
Rev Recd Date: 2003-01-13
Publish Date: 2003-06-20

Abstract

The optimized system used in RAPD reaction of Sargentodoxa cuneata must be established before analyzing the genetic diversity of Sargentodoxa cuneata .It is necessary to explore the reaction conditions .The genomic DNA of Sargentodoxa cuneata is extracted with improved SDS method and RAPD amplification is carried out .The effect of content of Mg , Dutp , DNA templates , primers and DNA polymerase on experimental results is tested and the optimal reaction system of RAPD for Sargentodoxa cuneata is determined as follows :1 Taq polymerase corresponding buffer (10 mmolL-1Tris HCl pH 9.0 , 50 mmolL-1KCl , 0.1 % Triton X-100 , 1.5 mmolL-1MgCl2), 25.01 10-9mols-1Taq DNA polymerase , 10 ng template DNA , 15 pmol primer , 2.00 gL-1BSA .0.15 mmolL-1dATP , dCTP , dGTP , dTTP for each in total 15 L reaction volume .[ Ch , 4 fig .2 tab .8 ref .]
- Sargentodoxa cuneata,
- RAPD,
- chemical composition,
- medicinal plant

Relative Article

[1]	LI Yuanyuan, ZHANG Shuangyan, WANG Chuangui, FANG Xuqin. Chemical composition, fiber morphology, and pulping properties of logging residues in Phyllostachys edulis . Journal of Zhejiang A&F University, 2019, 36(2): 219-226. doi: 10.11833/j.issn.2095-0756.2019.02.002
[2]	LIU Yunhui, LI Shen, WANG Yang, YANG Jian, ZHOU Wei, SHEN Yafang. Research progress on the functions of GRAS transcription factors in medical plants . Journal of Zhejiang A&F University, 2019, 36(6): 1233-1240. doi: 10.11833/j.issn.2095-0756.2019.06.022
[3]	LI Chenchen, ZHOU Zaizhi, LIANG Kunnan, HUANG Guihua, YANG Guang. Physical and chemical properties of ecological forest soils using different agroforestry patterns of Chinese fir with medicinal plants . Journal of Zhejiang A&F University, 2018, 35(1): 51-59. doi: 10.11833/j.issn.2095-0756.2018.01.007
[4]	LUO Jiayan, WANG Ying, ZHONG Wenhan, LIU Haichong, JIANG Huojun. Establishment of RAPD and ISSR markers for wood identification of 16 species of Lauraceae . Journal of Zhejiang A&F University, 2017, 34(5): 942-948. doi: 10.11833/j.issn.2095-0756.2017.05.023
[5]	DONG Leiming, ZENG Yanru, WU Yufen, HUANG Yinzhi, WU Dong, DAI Wensheng. Variations in phenotypic traits and chemical compositions of seeds from a natural population in Torreya grandis . Journal of Zhejiang A&F University, 2014, 31(2): 224-230. doi: 10.11833/j.issn.2095-0756.2014.02.010
[6]	CHEN Chen, DENG Yu-he, XU Liao, ZHOU Yu, CHEN Min, WU Jing, WANG Xiang-ge, YANG Ying. Microstructure and chemical composition of compressed and carbonized poplar . Journal of Zhejiang A&F University, 2012, 29(5): 671-679. doi: 10.11833/j.issn.2095-0756.2012.05.006
[7]	LIN Jun-yang, MA Liang-jin, CHEN An-liang, ZHANG Li-qin. Chemical components and antifungal activities of extracts from the husk of Carya cathayensis . Journal of Zhejiang A&F University, 2009, 26(1): 100-104.
[8]	SU Wen-hui, GU Xiao-ping, MA Ling-fei, WU Xiao-li, YUE Jin-jun, ZHENG Ren-hong. Study on chemical compositions of Bambusa wenchouensis wood . Journal of Zhejiang A&F University, 2005, 22(2): 180-184.
[9]	TANG Ding-qin, INOUE Makoto, KONDO Akira, IDE Yuji. Genetic analysis of RAPDs in Chamaecyparis obtusa using partial diallel test . Journal of Zhejiang A&F University, 2004, 21(3): 235-242.
[10]	WANG Ji-yong, WANG Wen-quan, WU Hui-xiao. Illumination domino effect and its influence on height growth of medicinal plant in intercropping system of Populus tomentosa and medicinal plants . Journal of Zhejiang A&F University, 2003, 20(1): 17-22.
[11]	GE Min-ju, JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng, ZHANG Li-long. A primary study on the flavonoids from Sargentodoxa cuneata . Journal of Zhejiang A&F University, 2002, 19(4): 382-386.
[12]	CHEN Liang, ZHOU Zhi-xiu, SHU Ai-min, LIU Xiao. Comparison of organoleptic evaluation and chemical composition among three kinds of kudingcha and tea . Journal of Zhejiang A&F University, 2000, 17(3): 298-300.
[13]	ZHOU Zhi-xiu, CHEN Liang, LOU Lu-huan, LI Gen-you. Medicinal plants from Mount Longwang Nature Reserve in Zhejiang . Journal of Zhejiang A&F University, 2000, 17(4): 378-383.
[14]	LIANG Yue-rong, TANAKA Juni-chi, TAKEDA Yoshi-yuki. Study on diversity of tea germplasm by RAPD method . Journal of Zhejiang A&F University, 2000, 17(2): 215-218.
[15]	Liang Yuerong, Lu Jianliang, Shang Shuling. Effect of Tea Cuifasu on Chemical Composition and Quality of Tea. . Journal of Zhejiang A&F University, 1997, 14(2): 155-158.
[16]	Mao Yan. A Preliminary Study of Chemical Composition in Leaves of Phyllostachys praecox and Ph. prominens. . Journal of Zhejiang A&F University, 1997, 14(4): 410-414.
[17]	Yu Xuejun, Tian Jingxiang. Chemical Compositions of Fastgrowing Chinese Fir Wood Zhejianng. . Journal of Zhejiang A&F University, 1997, 14(4): 330-332.
[18]	Lai Chungen, Ma Yuhuan, Zhang Bin, Xu Weifu.. Chemical Composition of the Leaf-extracts of Indocalamus. . Journal of Zhejiang A&F University, 1995, 12(2): 161-165.
[19]	Wu Bingsheng, Xia Yufang, Fu Maoyi, Zhang Jiaxian, Zhou Wei.. Chemical Composition of Bambusa distegia Wood. . Journal of Zhejiang A&F University, 1995, 12(3): 281-285.
[20]	Liu Hong′e, Liu Li, Shi Hongguang, Feng Han, Han Yifan. Chemical Composition of Wood of Some Poplars . Journal of Zhejiang A&F University, 1995, 12(4): 343-346.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(1396) PDF downloads(174) Cited by()

Proportional views

Optimization of RAPD conditions of Sargentodoxa cuneata

1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;

2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

Funds:

国家自然科学基金资助项目(39870160)

Received Date: 2002-08-02
Rev Recd Date: 2003-01-13
Publish Date: 2003-06-20

Key words:

Abstract: The optimized system used in RAPD reaction of Sargentodoxa cuneata must be established before analyzing the genetic diversity of Sargentodoxa cuneata .It is necessary to explore the reaction conditions .The genomic DNA of Sargentodoxa cuneata is extracted with improved SDS method and RAPD amplification is carried out .The effect of content of Mg , Dutp , DNA templates , primers and DNA polymerase on experimental results is tested and the optimal reaction system of RAPD for Sargentodoxa cuneata is determined as follows :1 Taq polymerase corresponding buffer (10 mmolL-1Tris HCl pH 9.0 , 50 mmolL-1KCl , 0.1 % Triton X-100 , 1.5 mmolL-1MgCl2), 25.01 10-9mols-1Taq DNA polymerase , 10 ng template DNA , 15 pmol primer , 2.00 gL-1BSA .0.15 mmolL-1dATP , dCTP , dGTP , dTTP for each in total 15 L reaction volume .[ Ch , 4 fig .2 tab .8 ref .]

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

Citation:

JIN Ze-xin, LI Jun-min, ZHONG Zhang-cheng. Optimization of RAPD conditions of Sargentodoxa cuneata[J]. Journal of Zhejiang A&F University, 2003, 20(2): 141-145.

HTML

DownLoad: Full-Size Img PowerPoint

Return

Optimization of RAPD conditions of Sargentodoxa cuneata

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Optimization of RAPD conditions of Sargentodoxa cuneata

1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;

2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

HTML

Catalog

Optimization of RAPD conditions of Sargentodoxa cuneata

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Optimization of RAPD conditions of Sargentodoxa cuneata

1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China; 2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China

HTML

Catalog

Export File

Citation

Format

Content

1. Department of Biology , Taizhou University , Linhai 317000, Zhejiang , China;

2. .Faculty of Life Sciences, Southwest Normal University , Chongqing 400715 , China