Tissue isolation and mycelium growth characteristics of <em>Lactarius delicious</em>

ZHOU Gou-ying; LIU Jun-ang; LI Qian-ru

Volume 20 Issue 2

Jul. 2017

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ZHOU Gou-ying, LIU Jun-ang, LI Qian-ru. Tissue isolation and mycelium growth characteristics of Lactarius delicious[J]. Journal of Zhejiang A&F University, 2003, 20(2): 158-161.

Citation:

ZHOU Gou-ying, LIU Jun-ang, LI Qian-ru. Tissue isolation and mycelium growth characteristics of Lactarius delicious[J]. Journal of Zhejiang A&F University, 2003, 20(2): 158-161.

Tissue isolation and mycelium growth characteristics of Lactarius delicious

1.
College of Resources and Environment , CSFU, Zhuzhou 412006 , Hunan, China;
2.
School of Life Sciences, Zhanjiang Ocean University , Zhanjiang 524000 , Guangdong , China

Received Date: 2002-12-24
Rev Recd Date: 2003-03-04
Publish Date: 2003-06-20

Abstract

The tissues on different positions of 6 basidiocarps of different sources are isolated with 5 kinds of culture media .The best medium for tissue isolation of L .delicious is PDA culture medium and 8 well-developed mycelia are singled out .The research of growth characteristics of mycelium A1 indicates that the mycelia can absorb all the carbon sucrose tested .The sucrose is the best utilized carbon nutrition , the glucose is the better and the fructose is the worst .As for the utilization of nitrogen source , of organic nitrogen , single nitrogen is better than compound nitrogen ;and urea cant be utilized .Of inorganic nitrogen , ammonium nitrogen is better than nitrate nitrogen , ammonium sulfate has the best effect and nitrite cant be utilized .Mycelia have strong adaptability to salt concentration and can grow when the salt concentration is 14 gL-1 .The optimum salt concentration for mycelia growth ranges from 0 to 6 gL-1 .The range of temperature for growth is 7 ～ 37 ℃.The optimum temperature for mycelia growth ranges from 15 to 30 ℃.The optimum temperature for growth is 27 ℃.The mycelia prefer neutral and acid environment .The pH values suitable for growth is 5 ～ 7 and the optimum one is 6.5 .[ Ch , 2 fig .4 tab .7 ref .]
- edible fungi,
- Lactarius delicious,
- tissue isolation,
- mycelium,
- growth characteristics

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Tissue isolation and mycelium growth characteristics of Lactarius delicious

1. College of Resources and Environment , CSFU, Zhuzhou 412006 , Hunan, China;

2. School of Life Sciences, Zhanjiang Ocean University , Zhanjiang 524000 , Guangdong , China

Received Date: 2002-12-24

Rev Recd Date: 2003-03-04

Publish Date: 2003-06-20

Key words:

Abstract: The tissues on different positions of 6 basidiocarps of different sources are isolated with 5 kinds of culture media .The best medium for tissue isolation of L .delicious is PDA culture medium and 8 well-developed mycelia are singled out .The research of growth characteristics of mycelium A1 indicates that the mycelia can absorb all the carbon sucrose tested .The sucrose is the best utilized carbon nutrition , the glucose is the better and the fructose is the worst .As for the utilization of nitrogen source , of organic nitrogen , single nitrogen is better than compound nitrogen ;and urea cant be utilized .Of inorganic nitrogen , ammonium nitrogen is better than nitrate nitrogen , ammonium sulfate has the best effect and nitrite cant be utilized .Mycelia have strong adaptability to salt concentration and can grow when the salt concentration is 14 gL-1 .The optimum salt concentration for mycelia growth ranges from 0 to 6 gL-1 .The range of temperature for growth is 7 ～ 37 ℃.The optimum temperature for mycelia growth ranges from 15 to 30 ℃.The optimum temperature for growth is 27 ℃.The mycelia prefer neutral and acid environment .The pH values suitable for growth is 5 ～ 7 and the optimum one is 6.5 .[ Ch , 2 fig .4 tab .7 ref .]

Tissue isolation and mycelium growth characteristics of Lactarius delicious

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通讯作者: 陈斌, bchen63@163.com

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Tissue isolation and mycelium growth characteristics of Lactarius delicious

1. College of Resources and Environment , CSFU, Zhuzhou 412006 , Hunan, China;

2. School of Life Sciences, Zhanjiang Ocean University , Zhanjiang 524000 , Guangdong , China

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Tissue isolation and mycelium growth characteristics of Lactarius delicious

1. College of Resources and Environment , CSFU, Zhuzhou 412006 , Hunan, China; 2. School of Life Sciences, Zhanjiang Ocean University , Zhanjiang 524000 , Guangdong , China

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1. College of Resources and Environment , CSFU, Zhuzhou 412006 , Hunan, China;

2. School of Life Sciences, Zhanjiang Ocean University , Zhanjiang 524000 , Guangdong , China