A preliminary study on induction of callus in <em>Carya cathayensis</em>

ZHU Yu-qiu; LIAO Wang-yi; HUANG Jian-qin; SUN Xiao-ping

Volume 18 Issue 2

Jul. 2017

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ZHU Yu-qiu, LIAO Wang-yi, HUANG Jian-qin, SUN Xiao-ping. A preliminary study on induction of callus in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2001, 18(2): 115-118.

Citation:

ZHU Yu-qiu, LIAO Wang-yi, HUANG Jian-qin, SUN Xiao-ping. A preliminary study on induction of callus in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2001, 18(2): 115-118.

A preliminary study on induction of callus in Carya cathayensis

1.
Department of Resources and Environment, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;
2.
Jinhua Branch of Zhejiang Senhe Seed Industry , Jinhua 321022, Zhejiang , China;
3.
Nursery of Hangzhou Garden and Cultural Relic Burean, Hangzhou 310023, Zhejiang , China

Funds:

浙江省自然科学基金资助项目(300011)

Received Date: 2000-12-21
Rev Recd Date: 2001-02-20
Publish Date: 2001-06-20

Abstract

Effects of such factors as ratios of different plant hormones , mass concentrations of surose , the part from which explants were obtained and the content of tannins on the formation of callus in the tissue culture of Carya cathayensis were studied by means of an orthogonal design .Its showed that WPM+BA 8.0 mgL-1 +IBA 2.0 mgL-1 +GA 1.0 mgL-1 +sucrose 30 mgL-1 was an optimal medium ;the portion between apical buds and the 4th bud was suitable to induce callus in Carya cathayensis ;and the formation rate of callus for loci of the buds was not correlated with the content of tannins , but in correlation with the loci where callus was formed .In addition , the smaller the content of tannins , the farther the locus where callus formed was from the cut .The key of raising survival rate of grafting was the selection of scions and the proportions of hormones to be used in grafting .
- Carya cathayensis,
- tissue culture,
- callus,
- tannin,
- grafting

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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A preliminary study on induction of callus in Carya cathayensis

1. Department of Resources and Environment, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. Jinhua Branch of Zhejiang Senhe Seed Industry , Jinhua 321022, Zhejiang , China;

3. Nursery of Hangzhou Garden and Cultural Relic Burean, Hangzhou 310023, Zhejiang , China

Funds:

浙江省自然科学基金资助项目(300011)

Received Date: 2000-12-21

Rev Recd Date: 2001-02-20

Publish Date: 2001-06-20

Key words:

Abstract: Effects of such factors as ratios of different plant hormones , mass concentrations of surose , the part from which explants were obtained and the content of tannins on the formation of callus in the tissue culture of Carya cathayensis were studied by means of an orthogonal design .Its showed that WPM+BA 8.0 mgL-1 +IBA 2.0 mgL-1 +GA 1.0 mgL-1 +sucrose 30 mgL-1 was an optimal medium ;the portion between apical buds and the 4th bud was suitable to induce callus in Carya cathayensis ;and the formation rate of callus for loci of the buds was not correlated with the content of tannins , but in correlation with the loci where callus was formed .In addition , the smaller the content of tannins , the farther the locus where callus formed was from the cut .The key of raising survival rate of grafting was the selection of scions and the proportions of hormones to be used in grafting .

A preliminary study on induction of callus in Carya cathayensis

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通讯作者: 陈斌, bchen63@163.com

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A preliminary study on induction of callus in Carya cathayensis

1. Department of Resources and Environment, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. Jinhua Branch of Zhejiang Senhe Seed Industry , Jinhua 321022, Zhejiang , China;

3. Nursery of Hangzhou Garden and Cultural Relic Burean, Hangzhou 310023, Zhejiang , China

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A preliminary study on induction of callus in Carya cathayensis

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通讯作者: 陈斌, bchen63@163.com

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A preliminary study on induction of callus in Carya cathayensis

1. Department of Resources and Environment, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China; 2. Jinhua Branch of Zhejiang Senhe Seed Industry , Jinhua 321022, Zhejiang , China; 3. Nursery of Hangzhou Garden and Cultural Relic Burean, Hangzhou 310023, Zhejiang , China

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1. Department of Resources and Environment, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. Jinhua Branch of Zhejiang Senhe Seed Industry , Jinhua 321022, Zhejiang , China;

3. Nursery of Hangzhou Garden and Cultural Relic Burean, Hangzhou 310023, Zhejiang , China