Volume 18 Issue 2
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Article Navigation >  Journal of Zhejiang A&F University  > 2001  >  18(2): 115-118

WANG Qianqian, JIANG Qini, FU Jianxin, et al. Screening reference genes of Osmanthus fragrans with differing photoperiod and temperature treatments[J]. Journal of Zhejiang A&F University, 2019, 36(5): 928-934. DOI: 10.11833/j.issn.2095-0756.2019.05.011
Citation: ZHU Yu-qiu, LIAO Wang-yi, HUANG Jian-qin, et al. A preliminary study on induction of callus in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2001, 18(2): 115-118.
shu

A preliminary study on induction of callus in Carya cathayensis

  • 1.

    Department of Resources and Environment, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

  • 2.

    Jinhua Branch of Zhejiang Senhe Seed Industry , Jinhua 321022, Zhejiang , China;

  • 3.

    Nursery of Hangzhou Garden and Cultural Relic Burean, Hangzhou 310023, Zhejiang , China

Funds:

浙江省自然科学基金资助项目(300011)

  • Received Date: 2000-12-21
  • Rev Recd Date: 2001-02-20
  • Publish Date: 2001-06-20
  • Effects of such factors as ratios of different plant hormones , mass concentrations of surose , the part from which explants were obtained and the content of tannins on the formation of callus in the tissue culture of Carya cathayensis were studied by means of an orthogonal design .Its showed that WPM+BA 8.0 mgL-1 +IBA 2.0 mgL-1 +GA 1.0 mgL-1 +sucrose 30 mgL-1 was an optimal medium ;the portion between apical buds and the 4th bud was suitable to induce callus in Carya cathayensis ;and the formation rate of callus for loci of the buds was not correlated with the content of tannins , but in correlation with the loci where callus was formed .In addition , the smaller the content of tannins , the farther the locus where callus formed was from the cut .The key of raising survival rate of grafting was the selection of scions and the proportions of hormones to be used in grafting .
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A preliminary study on induction of callus in Carya cathayensis

  • 1. Department of Resources and Environment, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;
  • 2. Jinhua Branch of Zhejiang Senhe Seed Industry , Jinhua 321022, Zhejiang , China;
  • 3. Nursery of Hangzhou Garden and Cultural Relic Burean, Hangzhou 310023, Zhejiang , China
Funds:

浙江省自然科学基金资助项目(300011)

  • Received Date: 2000-12-21
  • Rev Recd Date: 2001-02-20
  • Publish Date: 2001-06-20

Abstract: Effects of such factors as ratios of different plant hormones , mass concentrations of surose , the part from which explants were obtained and the content of tannins on the formation of callus in the tissue culture of Carya cathayensis were studied by means of an orthogonal design .Its showed that WPM+BA 8.0 mgL-1 +IBA 2.0 mgL-1 +GA 1.0 mgL-1 +sucrose 30 mgL-1 was an optimal medium ;the portion between apical buds and the 4th bud was suitable to induce callus in Carya cathayensis ;and the formation rate of callus for loci of the buds was not correlated with the content of tannins , but in correlation with the loci where callus was formed .In addition , the smaller the content of tannins , the farther the locus where callus formed was from the cut .The key of raising survival rate of grafting was the selection of scions and the proportions of hormones to be used in grafting .

WANG Qianqian, JIANG Qini, FU Jianxin, et al. Screening reference genes of Osmanthus fragrans with differing photoperiod and temperature treatments[J]. Journal of Zhejiang A&F University, 2019, 36(5): 928-934. DOI: 10.11833/j.issn.2095-0756.2019.05.011
Citation: ZHU Yu-qiu, LIAO Wang-yi, HUANG Jian-qin, et al. A preliminary study on induction of callus in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2001, 18(2): 115-118.
shu

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