Techniques of tissue culture and rapid propagation for <em>Anoectochilus formosanus</em>

HE Yun-fang; YANG Xia; YU You-xiang; QIU Li-zhen; JIANG Sheng-de

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Volume 16 Issue 2

Mar. 2019

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Article Navigation > Journal of Zhejiang A&F University > 1999 > 16(2): 170-174

HE Yun-fang, YANG Xia, YU You-xiang, QIU Li-zhen, JIANG Sheng-de. Techniques of tissue culture and rapid propagation for Anoectochilus formosanus[J]. Journal of Zhejiang A&F University, 1999, 16(2): 170-174.

Citation:

HE Yun-fang, YANG Xia, YU You-xiang, QIU Li-zhen, JIANG Sheng-de. Techniques of tissue culture and rapid propagation for Anoectochilus formosanus[J]. Journal of Zhejiang A&F University, 1999, 16(2): 170-174.

Techniques of tissue culture and rapid propagation for Anoectochilus formosanus

1.
Seed and Seedling Station of Zhejiang , Hangzhou 310004, China;
2.
Agriculture Research Institute of Hangzhou, Hangzhou 310008, China

Received Date: 1998-05-12
Rev Recd Date: 1998-09-20
Publish Date: 1999-06-20

Abstract

The cluster shoots and roots induced from stem segments of Anoectochilus formosanus in tissue cultures was studied .The results showed that B5 and MS media , BA , NAA , GA , with the concentrations of 4.0 mgL-1 , 0.3 mgL-1 , 0.3 mgL-1 respectively , and LB were suitable for cluster buds generating and growing .1/10MS medium , 20 gL-1 sucrose and 0.5mgL-1NAA were suitable for roots , and the segments vertically put on the surface of the medium rooted better .
- Anoectochilus formosanus,
- tissue cultures,
- rapid propagation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Techniques of tissue culture and rapid propagation for Anoectochilus formosanus

1. Seed and Seedling Station of Zhejiang , Hangzhou 310004, China;

2. Agriculture Research Institute of Hangzhou, Hangzhou 310008, China

Received Date: 1998-05-12
Rev Recd Date: 1998-09-20
Publish Date: 1999-06-20

Key words:

Abstract: The cluster shoots and roots induced from stem segments of Anoectochilus formosanus in tissue cultures was studied .The results showed that B5 and MS media , BA , NAA , GA , with the concentrations of 4.0 mgL-1 , 0.3 mgL-1 , 0.3 mgL-1 respectively , and LB were suitable for cluster buds generating and growing .1/10MS medium , 20 gL-1 sucrose and 0.5mgL-1NAA were suitable for roots , and the segments vertically put on the surface of the medium rooted better .

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Techniques of tissue culture and rapid propagation for Anoectochilus formosanus

1. Seed and Seedling Station of Zhejiang , Hangzhou 310004, China;

2. Agriculture Research Institute of Hangzhou, Hangzhou 310008, China

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Techniques of tissue culture and rapid propagation for Anoectochilus formosanus

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通讯作者: 陈斌, bchen63@163.com

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Techniques of tissue culture and rapid propagation for Anoectochilus formosanus

1. Seed and Seedling Station of Zhejiang , Hangzhou 310004, China; 2. Agriculture Research Institute of Hangzhou, Hangzhou 310008, China

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1. Seed and Seedling Station of Zhejiang , Hangzhou 310004, China;

2. Agriculture Research Institute of Hangzhou, Hangzhou 310008, China