Volume 25 Issue 3
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LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.
Citation: LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.

Houttuynia cordata analysis with RAPD markers

  • Received Date: 2007-09-27
  • Rev Recd Date: 2008-01-21
  • Publish Date: 2008-06-10
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Houttuynia cordata analysis with RAPD markers

Abstract: To explain the genetic relationship and genetic diversity of Houttuynia cordata, 24 samples from throughout China were analyzed using polymerase chain reaction(PCR) with random amplified polymorphic DNA(RAPD) molecular markers having 23 polymorphic primers screened from 160 RAPD primers. Then a cluster analysis was conducted. Results showed that 128 polymorphic bands were determined from the 165 clear bands accounting for 77.58%. From each primer, with an average of 7.1 bands, 5.5 polymorphic bands were amplified. The cluster analysis divided samples into 3 types according to their genetic distance, which got the same result with their morphological and biological characteristics. Thus, genetic diversity was high within H. cordata. These results will lay a theoretical foundation for breeding new varieties of H. cordata.

LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.
Citation: LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.

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