<i>Houttuynia cordata</i> analysis with RAPD markers

LAN Yun-long; WU Ling-shang; QIU Bo-yin; GAO Yan-hui; SI Jin-ping

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Volume 25 Issue 3

Jun. 2018

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Article Navigation > Journal of Zhejiang A&F University > 2008 > 25(3): 309-313

LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.

Citation:

LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.

Houttuynia cordata analysis with RAPD markers

1.
Forestry Science Institution of Lishui City, Lishui 323500, Zhejiang, China;
2.
Centre for Research and Development of Natural Medicines, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

Received Date: 2007-09-27
Rev Recd Date: 2008-01-21
Publish Date: 2008-06-10

Abstract

To explain the genetic relationship and genetic diversity of Houttuynia cordata, 24 samples from throughout China were analyzed using polymerase chain reaction(PCR) with random amplified polymorphic DNA(RAPD) molecular markers having 23 polymorphic primers screened from 160 RAPD primers. Then a cluster analysis was conducted. Results showed that 128 polymorphic bands were determined from the 165 clear bands accounting for 77.58%. From each primer, with an average of 7.1 bands, 5.5 polymorphic bands were amplified. The cluster analysis divided samples into 3 types according to their genetic distance, which got the same result with their morphological and biological characteristics. Thus, genetic diversity was high within H. cordata. These results will lay a theoretical foundation for breeding new varieties of H. cordata.
- botany,
- Houttuynia cordata,
- RAPD-PCR,
- genetic distance

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Houttuynia cordata analysis with RAPD markers

1. Forestry Science Institution of Lishui City, Lishui 323500, Zhejiang, China;

2. Centre for Research and Development of Natural Medicines, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

Received Date: 2007-09-27
Rev Recd Date: 2008-01-21
Publish Date: 2008-06-10

Key words:

Abstract: To explain the genetic relationship and genetic diversity of Houttuynia cordata, 24 samples from throughout China were analyzed using polymerase chain reaction(PCR) with random amplified polymorphic DNA(RAPD) molecular markers having 23 polymorphic primers screened from 160 RAPD primers. Then a cluster analysis was conducted. Results showed that 128 polymorphic bands were determined from the 165 clear bands accounting for 77.58%. From each primer, with an average of 7.1 bands, 5.5 polymorphic bands were amplified. The cluster analysis divided samples into 3 types according to their genetic distance, which got the same result with their morphological and biological characteristics. Thus, genetic diversity was high within H. cordata. These results will lay a theoretical foundation for breeding new varieties of H. cordata.

LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.

Citation:

LAN Yun-long, WU Ling-shang, QIU Bo-yin, GAO Yan-hui, SI Jin-ping. Houttuynia cordata analysis with RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 309-313.

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Houttuynia cordata analysis with RAPD markers

1. Forestry Science Institution of Lishui City, Lishui 323500, Zhejiang, China;

2. Centre for Research and Development of Natural Medicines, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

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Houttuynia cordata analysis with RAPD markers

1. Forestry Science Institution of Lishui City, Lishui 323500, Zhejiang, China; 2. Centre for Research and Development of Natural Medicines, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

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1. Forestry Science Institution of Lishui City, Lishui 323500, Zhejiang, China;

2. Centre for Research and Development of Natural Medicines, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China