Micropropagation of <i>Bambusa oldhami</i> f. <i>striata</i> by tissue culture

CHEN Yi-han; GUI Ren-yi; LIN Xin-chun; YANG Hai-yun; HUANG Li-chun

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Volume 25 Issue 3

Jun. 2018

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Article Navigation > Journal of Zhejiang A&F University > 2008 > 25(3): 397-400

CHEN Yi-han, GUI Ren-yi, LIN Xin-chun, YANG Hai-yun, HUANG Li-chun. Micropropagation of Bambusa oldhami f. striata by tissue culture[J]. Journal of Zhejiang A&F University, 2008, 25(3): 397-400.

Citation:

CHEN Yi-han, GUI Ren-yi, LIN Xin-chun, YANG Hai-yun, HUANG Li-chun. Micropropagation of Bambusa oldhami f. striata by tissue culture[J]. Journal of Zhejiang A&F University, 2008, 25(3): 397-400.

Micropropagation of Bambusa oldhami f. striata by tissue culture

1.
The Key Laboratory for Modern Sivilcultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

Received Date: 2007-10-22
Rev Recd Date: 2008-03-04
Publish Date: 2008-06-10

Abstract

Bambusa oldhami f. striata is a forma of Bambusa oldhami, having good ornamental value. This aim is to establish a tissue culture system for its commercial propagation. The new tender lateral bud were taken as the explants, Murashige and Skoog(MS) medium +30 g·L-1 sugar + 2.5 g·L-1Gelrite was the basic medium, and 6-Benzylaminopurine(BA) (0, 0.30, 1.00, 3.00, 10.00 mg·L-1) and thidiazuron(TDZ) (0, 0.01, 0.10, 1.00 mg·L-1) were added respectively to determine suitable concentration for tissue culture. The results showed that the shoot proliferation rate gradually increased with the concentration increasing of BA or TDZ, the effect of TDZ was better than BA, but the height growth of shoot was suppressed. 3.00 mg·L-1 BA+0.10 mg·L-1 TDZ was the best combination for shoot proliferation and shoot height growth. Declining or removing the cytokine could promote rooting.
- botany,
- Bambusa oldhami f. striata,
- tissue culture,
- vegetative propagation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Micropropagation of Bambusa oldhami f. striata by tissue culture

1. The Key Laboratory for Modern Sivilcultural Technology of Zhejiang Province, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China

Received Date: 2007-10-22
Rev Recd Date: 2008-03-04
Publish Date: 2008-06-10

Key words:

Abstract: Bambusa oldhami f. striata is a forma of Bambusa oldhami, having good ornamental value. This aim is to establish a tissue culture system for its commercial propagation. The new tender lateral bud were taken as the explants, Murashige and Skoog(MS) medium +30 g·L-1 sugar + 2.5 g·L-1Gelrite was the basic medium, and 6-Benzylaminopurine(BA) (0, 0.30, 1.00, 3.00, 10.00 mg·L-1) and thidiazuron(TDZ) (0, 0.01, 0.10, 1.00 mg·L-1) were added respectively to determine suitable concentration for tissue culture. The results showed that the shoot proliferation rate gradually increased with the concentration increasing of BA or TDZ, the effect of TDZ was better than BA, but the height growth of shoot was suppressed. 3.00 mg·L-1 BA+0.10 mg·L-1 TDZ was the best combination for shoot proliferation and shoot height growth. Declining or removing the cytokine could promote rooting.

CHEN Yi-han, GUI Ren-yi, LIN Xin-chun, YANG Hai-yun, HUANG Li-chun. Micropropagation of Bambusa oldhami f. striata by tissue culture[J]. Journal of Zhejiang A&F University, 2008, 25(3): 397-400.

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