Polyploidy induction in <EM>Zantedeschia elliottiana</EM>

LIANG Guo-lu; </sup>

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Volume 25 Issue 5

Oct. 2008

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Article Navigation > Journal of Zhejiang A&F University > 2008 > 25(5): 630-634

LIANG Guo-lu, . Polyploidy induction in Zantedeschia elliottiana[J]. Journal of Zhejiang A&F University, 2008, 25(5): 630-634.

Citation:

LIANG Guo-lu, . Polyploidy induction in Zantedeschia elliottiana[J]. Journal of Zhejiang A&F University, 2008, 25(5): 630-634.

Polyploidy induction in Zantedeschia elliottiana

LIANG Guo-lu^2
,,

1.
School of Agriculture and Food Science，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;
2.
College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China

More Information

Corresponding author: LIANG Guo-lu; LIANG Guo-lu
Publish Date: 2008-10-20

Abstract

To determine colchicine’s best concentration and treating time for inducing polyploidy in colorful calla lily，test materials with yellow cultivar of bulbs from Zantedeschia elliottiana ‘Black Magic’，were induced in vitro to form small buds from callus. Then，the small buds were soaked in the chemical reagent colchicine of 0.2，0.5 and 1.0 g·L －1 for 24，48 or 72 hours with 30 replications to induce polyploidy. Then，count the number of chromosome，and compare morphologic difference between the polyploidy and the diploid. Results showed that soaking small buds in 0.5 g·L －1 of colchicine for 48 hours could successfully induce tetraploid with an induction rate of 13.3%. The tetraploid were stronger and had thicker and larger leaves，larger guard cells，more chloroplasts，and less stoma density than the diploid. The tetraploid would mend the pace of new breed varieties.［Ch，3 fig. 3 tab. 18 ref.］
- horticulture,
- Zantedeschia elliottiana,
- tissue culture,
- colchicine,
- polyploidy induction

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Polyploidy induction in Zantedeschia elliottiana

LIANG Guo-lu^2,,

1. School of Agriculture and Food Science，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;

2. College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China

Corresponding author: LIANG Guo-lu; LIANG Guo-lu

Publish Date: 2008-10-20

Key words:

Abstract: To determine colchicine’s best concentration and treating time for inducing polyploidy in colorful calla lily，test materials with yellow cultivar of bulbs from Zantedeschia elliottiana ‘Black Magic’，were induced in vitro to form small buds from callus. Then，the small buds were soaked in the chemical reagent colchicine of 0.2，0.5 and 1.0 g·L －1 for 24，48 or 72 hours with 30 replications to induce polyploidy. Then，count the number of chromosome，and compare morphologic difference between the polyploidy and the diploid. Results showed that soaking small buds in 0.5 g·L －1 of colchicine for 48 hours could successfully induce tetraploid with an induction rate of 13.3%. The tetraploid were stronger and had thicker and larger leaves，larger guard cells，more chloroplasts，and less stoma density than the diploid. The tetraploid would mend the pace of new breed varieties.［Ch，3 fig. 3 tab. 18 ref.］

LIANG Guo-lu, . Polyploidy induction in Zantedeschia elliottiana[J]. Journal of Zhejiang A&F University, 2008, 25(5): 630-634.

Citation:

LIANG Guo-lu, . Polyploidy induction in Zantedeschia elliottiana[J]. Journal of Zhejiang A&F University, 2008, 25(5): 630-634.

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Polyploidy induction in Zantedeschia elliottiana

1. School of Agriculture and Food Science，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;

2. College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China

Corresponding author: LIANG Guo-lu; LIANG Guo-lu

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通讯作者: 陈斌, bchen63@163.com

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Polyploidy induction in Zantedeschia elliottiana

1. School of Agriculture and Food Science，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China; 2. College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China

Corresponding author: LIANG Guo-lu; LIANG Guo-lu

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1. School of Agriculture and Food Science，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;

2. College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China