Isolation and expression of a cadmium-resistant gene （<em>SaFer</em>） from<em> Sedum alfredii</em>

ZHAO Ting; HAN Xiaojiao; LIU Mingying; QIAO Guirong; JIANG Jing; JIANG Yancheng; ZHUO Renying

doi:10.11833/j.issn.2095-0756.2015.01.004

Volume 32 Issue 1

Jan. 2015

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2015 > 32(1): 25-32

ZHAO Ting, HAN Xiaojiao, LIU Mingying, QIAO Guirong, JIANG Jing, JIANG Yancheng, ZHUO Renying. Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii[J]. Journal of Zhejiang A&F University, 2015, 32(1): 25-32. doi: 10.11833/j.issn.2095-0756.2015.01.004

Citation:

ZHAO Ting, HAN Xiaojiao, LIU Mingying, QIAO Guirong, JIANG Jing, JIANG Yancheng, ZHUO Renying. Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii[J]. Journal of Zhejiang A&F University, 2015, 32(1): 25-32. doi: 10.11833/j.issn.2095-0756.2015.01.004

Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii

doi: 10.11833/j.issn.2095-0756.2015.01.004

1.
Department of Biology and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China;
2.
The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;
3.
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2014-03-01
Rev Recd Date: 2014-04-14
Publish Date: 2015-02-20

Abstract

Abiotic stress was a serious problem that affect plants growth and products. In plants, ferritin is a special iron storage protein closely related to stress. A cDNA from the Sedum alfredii cDNA library, designated as SaFer, was isolated and analyzed by homologous analysis and by Real-Time Reverse Transcription-PCR （qRT-PCR）. Then, cadmium-stress experiments were conducted to compare transgenic Arabidopsis thaliana overexpressed SaFer to a wild type. Results showed that SaFer cDNA was 1 117 bp long with an opening reading frame （ORF） of 759 bp. The ORF of SaFer encoded a polypeptide of 252 amino acids with a calculated molecular weight of about 27.8 kDa, and the homologous analysis showed that it was most closely related to ferritin of Malus domestica with 76% identities. The length of the genomic sequence of SaFer was 1 702 bp and contained 7 introns. Expression profiles in roots were analyzed by qRT-PCR and the results showed that the transcription level of SaFer was enhanced after 12 h cadmium stress. Transgenic Arabidopsis thaliana overexpressing SaFer displayed much higher Cd tolerance than the wild type which was further supported by physiological indexes such as SOD activity and electric conductivity. The results showed that SaFer could provide a glimpse into S. alfredii cadmium-tolerance and could contribute to the breeding of cadmium-tolerant plants. ［Ch，9 fig. 1 tab. 23 ref. ］
- botany,
- Sedum alfredii,
- ferritin,
- cadmium stress,
- expression change

Relative Article

[1]	WANG Shuwei, ZHOU Mingbing. Genome-wide identification of the ICE gene family in moso bamboo and its expression pattern under low temperature stress . Journal of Zhejiang A&F University, 2024, 41(3): 568-576. doi: 10.11833/j.issn.2095-0756.20230445
[2]	ZHUO Juan, HOU Dan, LIN Xinchun. Cloning and expression analysis of PhebHLH6 gene from Phyllostachys edulis . Journal of Zhejiang A&F University, 2023, 40(4): 731-737. doi: 10.11833/j.issn.2095-0756.20220553
[3]	LAN Zhixin, HOU Dan, WU Aimin, LIN Xinchun. Cloning and expression analysis of PeCIGRs gene from Phyllostachys edulis . Journal of Zhejiang A&F University, 2023, 40(5): 982-990. doi: 10.11833/j.issn.2095-0756.20220761
[4]	HONG Fanglei, LU Yao, YU Shijiao, HU Zhinuo, MIAO Yunfeng, ZHONG Shiwei, ZHAO Hongbo. Cloning and expression analysis of OfABFs gene in Osmanthus fragrans . Journal of Zhejiang A&F University, 2023, 40(3): 481-491. doi: 10.11833/j.issn.2095-0756.20220264
[5]	MENG Chaomin, GENG Feifei, QING Guixia, ZHANG Fuhou, LI Xuelin, LIU Fengju. Cloning and expression analysis of low phosphorus stress response gene GhGDPD1 in Gossypium hirsutum . Journal of Zhejiang A&F University, 2023, 40(4): 723-730. doi: 10.11833/j.issn.2095-0756.20220624
[6]	JIA Junwei, CHEN Zhenhua, LIAO Shiyan, LUO Wenxuan, XU Weijie, ZHONG Bin, MA Jiawei, YE Zhengqian, LIU Dan. Transfer coefficient and distribution characteristics of Cd in low absorption rice cultivars under Cd stress . Journal of Zhejiang A&F University, 2022, 39(5): 1059-1066. doi: 10.11833/j.issn.2095-0756.20210402
[7]	WANG Shaoliang, ZHANG Wenyu, GAO Zhimin, ZHOU Mingbing, YANG Kebin, SONG Xinzhang. Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis . Journal of Zhejiang A&F University, 2022, 39(3): 486-494. doi: 10.11833/j.issn.2095-0756.20210471
[8]	LOU Yongfeng, GAO Zhimin. Cloning and functional analysis of early light induced protein genes of Phyllostachys edulis . Journal of Zhejiang A&F University, 2021, 38(1): 93-102. doi: 10.11833/j.issn.2095-0756.20200237
[9]	WANG Nannan, DONG Bin, YANG Liyuan, ZHAO Hongbo. Cloning and expression analysis under adversity stress of 2 PmWRKY2 in Prunus mume . Journal of Zhejiang A&F University, 2021, 38(4): 812-819. doi: 10.11833/j.issn.2095-0756.20200706
[10]	MIAO Yunfeng, ZHOU Dan, DONG Bin, ZHAO Hongbo. Identification and expression analysis of OfNAC transcription factors in Osmanthus fragrans during flower opening stage . Journal of Zhejiang A&F University, 2021, 38(3): 433-444. doi: 10.11833/j.issn.2095-0756.20200474
[11]	LIU Bo, CHEN Ji, MA Jiawei, FANG Xianzhi, ZHAO Keli, LIU Dan, YE Zhengqian. Effects of AC electric field and soil moisture on phytoremediation of cadmium contaminated soil by mixed planting of Salix discolor and Sedum alfredii . Journal of Zhejiang A&F University, 2021, 38(6): 1238-1244. doi: 10.11833/j.issn.2095-0756.20200725
[12]	XIE Dezhi, WEI Zilu, ZHU Junyi, DU Ying, JIN Shuihu, YUE Chunlei. Physiological responses of Hygroryza aristata to cadmium stress . Journal of Zhejiang A&F University, 2020, 37(4): 683-692. doi: 10.11833/j.issn.2095-0756.20190407
[13]	LI Bingbing, LIU Guofeng, WEI Shu, HUANG Longquan, ZHANG Jianyun. Cloning and expression analysis of the tobacco NtPLR1 gene . Journal of Zhejiang A&F University, 2017, 34(4): 581-588. doi: 10.11833/j.issn.2095-0756.2017.04.003
[14]	HU Yangyong, MA Jiawei, YE Zhengqian, LIU Dan, ZHAO Keli. Research progress on using Sedum alfredii for remediation of heavy metal-contaminated soil . Journal of Zhejiang A&F University, 2014, 31(1): 136-144. doi: 10.11833/j.issn.2095-0756.2014.01.021
[15]	LI Zhen, LIU Mingying, HAN Xiaojiao, QIAO Guirong, JIANG Jing, XING Shiyan, ZHUO Renying. Characterization of a light-harvesting chlorophyll a/b binding protein (LHCB) gene, SaLhcb2, in Sedum alfredii . Journal of Zhejiang A&F University, 2014, 31(6): 838-846. doi: 10.11833/j.issn.2095-0756.2014.06.003
[16]	YANG Xihong, HUANG Youjun, CHEN Fangfang, HUANG Jianqin. Identification and expression of FLOWERING LOCUS C homologous genes in Carya cathayensis . Journal of Zhejiang A&F University, 2013, 30(1): 1-8. doi: 10.11833/j.issn.2095-0756.2013.01.001
[17]	MA Jin, LIU Zhi-gao, ZHENG Gang. Differential proteomics and its application in plant salt stress . Journal of Zhejiang A&F University, 2011, 28(1): 139-143. doi: 10.11833/j.issn.2095-0756.2011.01.022
[18]	SONG Hong－gai, JIANG Jing, QIAO Gui－rong, YANG Ye, ZHOU Jing, PAN Luan－yin, ZHUO Ren－ying. Plant stress－resistant gene cloning for Na and Cd using an INVSc1 yeast . Journal of Zhejiang A&F University, 2010, 27(6): 890-895. doi: 10.11833/j.issn.2095-0756.2010.06.014
[19]	ZHANG Yuan－yuan, DOU Chun－ying, YAO Fang1, YE Zheng－qian. Nitrogen application to enhance zinc and cadmium uptake by the hyperaccumulator Sedum alfredii . Journal of Zhejiang A&F University, 2010, 27(6): 831-838. doi: 10.11833/j.issn.2095-0756.2010.06.005
[20]	ZHAO Zhi-xin, QIAO Rui-fang, JI Kong-shu. Cadmium stress on growth and resistance of hybrid tulip trees：Liriodendron chinense × Liriodendron tulipifera . Journal of Zhejiang A&F University, 2009, 26(5): 667-673.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3376) PDF downloads(462) Cited by()

Proportional views

Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii

1. Department of Biology and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China;

2. The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;

3. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2014-03-01

Rev Recd Date: 2014-04-14

Publish Date: 2015-02-20

Key words:

Abstract: Abiotic stress was a serious problem that affect plants growth and products. In plants, ferritin is a special iron storage protein closely related to stress. A cDNA from the Sedum alfredii cDNA library, designated as SaFer, was isolated and analyzed by homologous analysis and by Real-Time Reverse Transcription-PCR （qRT-PCR）. Then, cadmium-stress experiments were conducted to compare transgenic Arabidopsis thaliana overexpressed SaFer to a wild type. Results showed that SaFer cDNA was 1 117 bp long with an opening reading frame （ORF） of 759 bp. The ORF of SaFer encoded a polypeptide of 252 amino acids with a calculated molecular weight of about 27.8 kDa, and the homologous analysis showed that it was most closely related to ferritin of Malus domestica with 76% identities. The length of the genomic sequence of SaFer was 1 702 bp and contained 7 introns. Expression profiles in roots were analyzed by qRT-PCR and the results showed that the transcription level of SaFer was enhanced after 12 h cadmium stress. Transgenic Arabidopsis thaliana overexpressing SaFer displayed much higher Cd tolerance than the wild type which was further supported by physiological indexes such as SOD activity and electric conductivity. The results showed that SaFer could provide a glimpse into S. alfredii cadmium-tolerance and could contribute to the breeding of cadmium-tolerant plants. ［Ch，9 fig. 1 tab. 23 ref. ］

Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii

doi: 10.11833/j.issn.2095-0756.2015.01.004

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii

doi: 10.11833/j.issn.2095-0756.2015.01.004

HTML

Catalog

Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii

doi: 10.11833/j.issn.2095-0756.2015.01.004

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Isolation and expression of a cadmium-resistant gene （SaFer） from Sedum alfredii

doi: 10.11833/j.issn.2095-0756.2015.01.004

HTML

Catalog

Export File

Citation

Format

Content