-
毛竹Phyllostachys edulis为禾本科Gramineae竹亚科Bambusoideae刚竹属Phyllostachys竹种,用途广泛,是一种可持续发展的重要资源。培育毛竹壮苗是提高实际造林成活率的重要前提[1]。目前,毛竹实生苗因成本低、便于引种等优势,已在生产上广泛利用。油菜素内酯(brassinosteroid,BR)是一种类似于动物甾醇类激素的天然产物,在极低浓度下就可以对植物的生长及发育起到显著的调节改善作用,被认为是继生长素、赤霉素、细胞分裂素、脱落酸、乙烯之后的第六大植物生长调节剂。MITCHELL等[2]首次从油菜Brassica napus花粉中提取油菜素内酯, 发现其极高的生物活性, 能引起菜豆Phaseolus vulgaris节间伸长、弯曲、裂开等生长反应。进一步的研究发现:油菜素内酯参与多种植物生理生长的过程,对植物的生长发育、光合特性、抗逆性、细胞发育和果实品质及产量等方面有重要的调节功能[3-5]。目前,关于外源油菜素内酯对毛竹生理特性影响鲜有报道。本研究以毛竹实生苗为材料,喷施不同质量浓度的油菜素内酯溶液,研究外源油菜素内酯对毛竹实生苗生理特性的影响,以期为培育毛竹壮苗提供理论依据。
-
由表1可知:随油菜素内酯质量浓度降低,毛竹实生苗株高、根长和叶面积均呈现先上升后下降的趋势。当油菜素内酯质量浓度在0.001 0 mg·L−1时毛竹实生苗株高达最高(P<0.05),较ck提高了11.0%,0.005 0 mg·L−1处理较ck提高了9.2%。0.001 0 mg·L−1处理较ck,其根长增加了13.4%,0.005 0和0.000 5 mg·L−1处理根长也均高于ck,但低于0.001 0 mg·L−1处理。除0.050 0 mg·L−1处理外,其余4组处理较ck均能显著增加毛竹实生苗叶面积(P<0.05),其中0.005 0 mg·L−1处理较ck效果最为显著;通过叶面喷施油菜素内酯,各处理与ck相比,0.010 0、0.005 0和0.001 0 mg·L−13种处理能显著增加毛竹实生苗全株总干质量(P<0.05),其中以0.005 0和0.001 0 mg·L−1处理为最佳,分别比 ck 增加了25.3%和26.8%。植株地上与地下部分干质量均随油菜素内酯质量浓度降低呈先上升后下降的趋势,且相较ck均有不同程度提升,其中0.005 0 mg·L−1较ck对植株地上部分干质量增加尤为显著(P<0.05),0.001 0 mg·L−1处理较ck对植株地下部分干质量提升尤为显著(P<0.05)。外源油菜素内酯处理各组与ck相比,均能在不同程度上降低毛竹实生苗的根冠比,说明毛竹实生苗地上部分营养生长较旺盛,生物量大。
表 1 不同质量浓度油菜素内酯处理毛竹实生苗生长指标
Table 1. Effect of different concentration BR on growth index of Ph. edulis seedlings
处理 株高/cm 根长/cm 叶面积/cm2 总干质量/g 地上部分干质量/g 地下部分干质量/g 根冠比 ck 27.69±1.39 c 29.83±0.85 c 11.58±0.22 c 1.38±0.09 c 0.91±0.09 c 0.47±0.03 b 0.52±0.07 a E1 29.93±1.78 ab 29.07±1.22 c 11.98±0.30 bc 1.50±0.12 bc 1.03±0.11 bc 0.48±0.04 b 0.47±0.06 ab E2 30.19±1.48 ab 29.81±0.74 c 13.39±0.57 a 1.71±0.21 a 1.20±0.19 ab 0.51±0.03 ab 0.43±0.05 b E3 30.24±1.95 ab 30.21±1.13 bc 13.68±0.27 a 1.73±0.15 a 1.22±0.15 a 0.50±0.05 ab 0.42±0.08 b E4 30.60±1.88 a 33.84±1.20 a 13.32±0.71 a 1.74±0.20 a 1.21±0.18 ab 0.54±0.04 a 0.45±0.07 ab E5 28.51±1.45 bc 31.10±1.24 b 12.88±0.42 ab 1.64±0.18 ab 1.11±0.19 ab 0.52±0.03 a 0.49±0.11 ab 说明:同列比较,不同字母表示处理间差异显著(P<0.05), 相同字母表示处理间差异不显著(P>0.05) -
由表2可知:各处理与ck相比均能显著增加毛竹实生苗叶绿素a的质量分数,但以0.005 0 mg·L−1处理叶绿素a质量分数增幅最大,比ck高11.6%。除0.010 0 mg·L−1处理叶绿素b质量分数差异不显著外,其余处理较ck均差异显著(P<0.05),但0.005 0和0.001 0 mg·L−1处理质量分数高于其他处理。5种质量浓度油菜素内酯处理间总叶绿素和类胡萝卜素质量分数差异均不显著,但相较于ck,差异均达到显著水平(P<0.05)。当质量浓度为0.005 0 mg·L−1时,总叶绿素质量分数高于其他处理,其次是0.001 0 mg·L−1;而0.001 0 mg·L−1处理下类胡萝卜素质量分数高于其他处理。由此表明:外源油菜素内酯处理可以提高毛竹实生苗光合色素水平,增强植株的光能利用率,从而提高植株光合能力。
表 2 不同质量浓度油菜素内酯处理毛竹实生苗光合色素质量分数
Table 2. Effect of different concentration BR on photosynthetic pigment content of Ph. edulis seedlings
处理 叶绿素a/(mg·g−1) 叶绿素b/(mg·g−1) 总叶绿素/(mg·g−1) 类胡萝卜素/(mg·g−1) ck 2.28±0.08 c 0.88±0.04 b 3.17±0.12 b 0.92±0.04 b E1 2.50±0.08 ab 1.01±0.06 a 3.51±0.14 a 1.06±0.04 a E2 2.41±0.11 b 0.96±0.08 ab 3.37±0.19 a 1.01±0.06 a E3 2.55±0.08 a 1.03±0.06 a 3.58±0.14 a 1.05±0.07 a E4 2.50±0.10 ab 1.03±0.07 a 3.53±0.17 a 1.07±0.05 a E5 2.50±0.08 ab 1.00±0.06 a 3.51±0.14 a 1.06±0.04 a 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05) 由表3可知:随着油菜素内酯质量浓度的降低,净光合速率、气孔导度、胞间二氧化碳摩尔分数和蒸腾速率均呈现先升高后降低的趋势。相较于其他处理,0.005 0和0.001 0 mg·L−1处理对净光合速率、气孔导度、胞间二氧化碳摩尔分数和蒸腾速率的影响较ck差异最为显著(P<0.05):0.005 0 mg·L−1处理分别提升26.7%、59.9%、14.1%和28.9%;0.001 0 mg·L−1处理分别提升22.7%、50.8%、16.1%和21.9%。说明外源油菜素内酯对毛竹实生苗光合能力具有促进作用,且随着油菜素内酯质量浓度的改变促进的效果发生变化。
表 3 不同质量浓度油菜素内酯处理毛竹实生苗光合参数
Table 3. Effect of different concentration BR on photosynthetic parameters of Ph. edulis seedlings
处理 净光合速率/(µmol·m−2·s−1) 气孔导度/(mol·m−2·s−1) 胞间二氧化碳摩尔分数/(µmol·mol−1) 蒸腾速率/(mmol·m−2·s−1) ck 6.87±0.21 c 0.051±0.005 d 211.47±15.86 c 1.36±0.08 c E1 7.07±0.21 c 0.069±0.005 c 231.57±13.77 ab 1.48±0.13 bc E2 7.53±0.32 b 0.074±0.005 bc 223.91±17.69 bc 1.56±0.16 b E3 8.70±0.33 a 0.082±0.004 a 241.37±10.34 ab 1.75±0.08 a E4 8.42±0.33 a 0.077±0.003 ab 245.53±9.62 a 1.66±0.13 ab E5 7.49±0.41 b 0.074±0.006 bc 234.66±11.15 ab 1.59±0.15 ab 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05) -
由表4可知:相较于ck,其他4种质量浓度处理的毛竹实生苗叶片的可溶性蛋白质质量分数均达到差异显著水平(P<0.05),随着喷施质量浓度的降低,可溶性蛋白质呈现先上升后下降的趋势,且在0.005 0和0.001 0 mg·L−1处理下达到最大值。叶面喷施条件下,除0.010 0和0.001 0 mg·L−1差异不显著外,其他处理与ck相比可溶性糖质量分数均达到显著水平(P<0.05),其中0.050 0与0.000 5 mg·L−1处理之间差异不显著,而0.005 0 mg·L−1处理后较ck增幅最大(P<0.05),增加了10.9%。随着油菜素内酯质量浓度的降低,毛竹实生苗淀粉质量分数也呈先上升后下降的趋势,0.005 0和0.001 0 mg·L−1处理较ck差异达显著水平(P<0.05),分别增加17.3%和16.9%。说明外源油菜素内酯溶液可以调节毛竹实生苗体内渗透调节物质的质量分数,有利于植株正常生长发育。
表 4 不同质量浓度油菜素内酯处理毛竹实生苗可溶性蛋白质、可溶性糖和淀粉质量分数
Table 4. Effect of different concentration BR on soluble protein,soluble sugar and amylum content of Ph. edulis seedlings
处理 可溶性蛋白质/(mg·g−1) 可溶性糖/(mg·g−1) 淀粉/(mg·g−1) 处理 可溶性蛋白质/(mg·g−1) 可溶性糖/(mg·g−1) 淀粉/(mg·g−1) ck 4.02±0.20 d 5.08±0.11 c 1.37±0.03 c E3 5.29±0.14 a 5.64±0.15 a 1.61±0.06 a E1 4.21±0.06 c 5.32±0.13 b 1.52±0.08 a E4 5.28±0.13 ab 5.17±0.20 bc 1.60±0.07 a E2 4.26±0.06 c 5.04±0.20 c 1.51±0.02 ab E5 5.10±0.08 ab 5.35±0.20 b 1.42±0.02 b 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05) -
由表5可知:超氧化物歧化酶和过氧化氢酶活性随油菜素内酯质量浓度降低呈现先上升后下降的趋势。当质量浓度在0.001 0~0.005 0 mg·L−1时,植株超氧化物歧化酶活性达到最大值,其中0.005 0 mg·L−1处理相较于ck增幅最大,提高了6.7%;过氧化氢酶活性则在0.005 0和0.001 0 mg·L−1处理下达到最大值。毛竹实生苗叶片的过氧化物酶活性,随油菜素内酯质量浓度的降低呈升高趋势,当质量浓度在0.000 5 mg·L−1时,植株过氧化物酶活性相较ck差异达显著水平(P<0.05),依次是0.005 0和0.001 0 mg·L−1。这些结果表明:毛竹实生苗叶片的抗氧化酶活性受外源油菜素内酯质量浓度影响,适宜质量浓度油菜素内酯可以提高抗氧化酶活性,增强植株抗逆性。
表 5 不同质量浓度油菜素内酯处理毛竹实生苗抗氧化酶活性
Table 5. Effect of different concentration BR on antioxidant enzyme activities of bamboo seedlings
组别 超氧化物歧化酶/
(×16.67 nkat·g−1)过氧化物酶/
(×16.67 nkat·g−1)过氧化氢酶/
(×16.67 nkat·g−1)组别 超氧化物歧化酶/
(×16.67 nkat·g−1)过氧化物酶/
(×16.67 nkat·g−1)过氧化氢酶/
(×16.67 nkat·g−1)ck 3 018.58±80.60 bc 43 031.11±2 231.96 c 46.81±1.47 c E3 3 219.58±106.30 a 49 306.67±1 573.26 ab 50.67±0.73 ab E1 2 978.00±102.19 c 46 871.11±1 625.14 b 46.96±2.57 c E4 3 202.99±99.55 a 48 937.78±2 994.76 ab 53.63±2.00 a E2 3 159.32±111.30 ab 46 404.44±1 779.30 b 48.44±0.73 bc E5 3 187.05±118.30 a 51 733.33±2 538.05 a 46.37±0.91 c 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05)
Effects of exogenous BR on physiological characteristics of Phyllostachys edulis seedlings
-
摘要:
目的 研究外源喷施油菜素内酯对毛竹Phyllostachys edulis生理特性的影响,为未来毛竹种植栽培及管理提供理论依据和技术参考。 方法 以毛竹实生苗为试材,通过水培试验,设置6种不同质量浓度油菜素内酯溶液处理[0(对照)、0.050 0、0.010 0、0.005 0、0.001 0、0.000 5 mg·L−1],比较处理间毛竹实生苗生长性状、光合色素、光合特性以及抗氧化系统等的差异。 结果 从整体上看,与对照相比,不同质量浓度的油菜素内酯在不同程度上对毛竹实生苗生长发育,对光合色素质量分数及光合能力起到提升作用。相较于对照,当油菜素内酯质量浓度为0.005 0 和0.001 0 mg·L−1时,毛竹实生苗超氧化物歧化酶活性分别提高6.7%和6.1%,过氧化物酶活性分别提高14.6%和13.7%,过氧化氢酶活性分别提高8.2%和14.6%,可溶性蛋白质质量分数分别增加31.5%和31.2%,淀粉质量分数分别增加17.3%和16.9%;而质量浓度为0.050 0、0.010 0及0.000 5 mg·L−1的油菜素内酯处理与0.005 0和0.001 0 mg·L−1质量浓度处理相比,对毛竹实生苗抗氧化性能与渗透调节能力的提升效果并不明显。 结论 油菜素内酯能提高毛竹实生苗光合能力,增强抗逆境能力,从而促进其营养生长,且当质量浓度在0.005 0~0.001 0 mg·L−1时效果最好。表5参42 Abstract:Objective This research aims to study the effects of exogenous brassinolide (BR) on physiological characteristics of Phyllostachys edulis, and to provide theoretical basis and technical reference for future cultivation and management of Ph. edulis. Method The seedlings of Ph. edulis were used as test materials, and 6 kinds of brassinolide solutions with different concentrations[0(ck), 0.050 0, 0.010 0, 0.005 0, 0.001 0, 0.000 5 mg·L−1] were set up through hydroponic culture experiment to compare the differences in the growth characteristics, photosynthetic pigment, photosynthetic characteristics and antioxidant system of the seedlings. Result On the whole, compared with ck, brassinolide with different mass concentrations could promote the growth and development of Ph. edulis seedlings to varying degrees, improve the quality of photosynthetic pigments and photosynthetic capacity, and promote the synthesis of osmotic adjustment substances. Compared with ck, when the mass concentration of brassinolide was 0.005 0 and 0.001 0 mg·L−1, the superoxide dismutase activity of bamboo seedlings increased by 6.7% and 6.1%, respectively, oxidase activity increased by 14.6% and 13.7%, and catalase activity increased by 8.2% and 14.6%, respectively. The mass fraction of soluble protein increased by 31.5% and 31.2%, and the mass fraction of starch increased by 17.3% and 16.9%, respectively. However, compared with 0.005 0 and 0.001 0 mg·L−1 treatment, brassinolide treated with mass concentration of 0.050 0, 0.010 0 and 0.000 5 mg·L−1 had no significant effect on antioxidant capacity and osmotic adjustment ability of Ph. edulis seedlings. Conclusion Brassinolide can improve the photosynthetic capacity and stress resistance of Ph. edulis seedlings, and promote nutrient growth. The best concentration of brassinolide is 0.005 0−0.001 0 mg·L−1.[Ch, 5 tab. 42 ref.] -
Key words:
- Phyllostachys edulis /
- brassinolide /
- nutrient growth /
- photosynthesis /
- antioxidant system
-
表 1 不同质量浓度油菜素内酯处理毛竹实生苗生长指标
Table 1. Effect of different concentration BR on growth index of Ph. edulis seedlings
处理 株高/cm 根长/cm 叶面积/cm2 总干质量/g 地上部分干质量/g 地下部分干质量/g 根冠比 ck 27.69±1.39 c 29.83±0.85 c 11.58±0.22 c 1.38±0.09 c 0.91±0.09 c 0.47±0.03 b 0.52±0.07 a E1 29.93±1.78 ab 29.07±1.22 c 11.98±0.30 bc 1.50±0.12 bc 1.03±0.11 bc 0.48±0.04 b 0.47±0.06 ab E2 30.19±1.48 ab 29.81±0.74 c 13.39±0.57 a 1.71±0.21 a 1.20±0.19 ab 0.51±0.03 ab 0.43±0.05 b E3 30.24±1.95 ab 30.21±1.13 bc 13.68±0.27 a 1.73±0.15 a 1.22±0.15 a 0.50±0.05 ab 0.42±0.08 b E4 30.60±1.88 a 33.84±1.20 a 13.32±0.71 a 1.74±0.20 a 1.21±0.18 ab 0.54±0.04 a 0.45±0.07 ab E5 28.51±1.45 bc 31.10±1.24 b 12.88±0.42 ab 1.64±0.18 ab 1.11±0.19 ab 0.52±0.03 a 0.49±0.11 ab 说明:同列比较,不同字母表示处理间差异显著(P<0.05), 相同字母表示处理间差异不显著(P>0.05) 表 2 不同质量浓度油菜素内酯处理毛竹实生苗光合色素质量分数
Table 2. Effect of different concentration BR on photosynthetic pigment content of Ph. edulis seedlings
处理 叶绿素a/(mg·g−1) 叶绿素b/(mg·g−1) 总叶绿素/(mg·g−1) 类胡萝卜素/(mg·g−1) ck 2.28±0.08 c 0.88±0.04 b 3.17±0.12 b 0.92±0.04 b E1 2.50±0.08 ab 1.01±0.06 a 3.51±0.14 a 1.06±0.04 a E2 2.41±0.11 b 0.96±0.08 ab 3.37±0.19 a 1.01±0.06 a E3 2.55±0.08 a 1.03±0.06 a 3.58±0.14 a 1.05±0.07 a E4 2.50±0.10 ab 1.03±0.07 a 3.53±0.17 a 1.07±0.05 a E5 2.50±0.08 ab 1.00±0.06 a 3.51±0.14 a 1.06±0.04 a 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05) 表 3 不同质量浓度油菜素内酯处理毛竹实生苗光合参数
Table 3. Effect of different concentration BR on photosynthetic parameters of Ph. edulis seedlings
处理 净光合速率/(µmol·m−2·s−1) 气孔导度/(mol·m−2·s−1) 胞间二氧化碳摩尔分数/(µmol·mol−1) 蒸腾速率/(mmol·m−2·s−1) ck 6.87±0.21 c 0.051±0.005 d 211.47±15.86 c 1.36±0.08 c E1 7.07±0.21 c 0.069±0.005 c 231.57±13.77 ab 1.48±0.13 bc E2 7.53±0.32 b 0.074±0.005 bc 223.91±17.69 bc 1.56±0.16 b E3 8.70±0.33 a 0.082±0.004 a 241.37±10.34 ab 1.75±0.08 a E4 8.42±0.33 a 0.077±0.003 ab 245.53±9.62 a 1.66±0.13 ab E5 7.49±0.41 b 0.074±0.006 bc 234.66±11.15 ab 1.59±0.15 ab 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05) 表 4 不同质量浓度油菜素内酯处理毛竹实生苗可溶性蛋白质、可溶性糖和淀粉质量分数
Table 4. Effect of different concentration BR on soluble protein,soluble sugar and amylum content of Ph. edulis seedlings
处理 可溶性蛋白质/(mg·g−1) 可溶性糖/(mg·g−1) 淀粉/(mg·g−1) 处理 可溶性蛋白质/(mg·g−1) 可溶性糖/(mg·g−1) 淀粉/(mg·g−1) ck 4.02±0.20 d 5.08±0.11 c 1.37±0.03 c E3 5.29±0.14 a 5.64±0.15 a 1.61±0.06 a E1 4.21±0.06 c 5.32±0.13 b 1.52±0.08 a E4 5.28±0.13 ab 5.17±0.20 bc 1.60±0.07 a E2 4.26±0.06 c 5.04±0.20 c 1.51±0.02 ab E5 5.10±0.08 ab 5.35±0.20 b 1.42±0.02 b 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05) 表 5 不同质量浓度油菜素内酯处理毛竹实生苗抗氧化酶活性
Table 5. Effect of different concentration BR on antioxidant enzyme activities of bamboo seedlings
组别 超氧化物歧化酶/
(×16.67 nkat·g−1)过氧化物酶/
(×16.67 nkat·g−1)过氧化氢酶/
(×16.67 nkat·g−1)组别 超氧化物歧化酶/
(×16.67 nkat·g−1)过氧化物酶/
(×16.67 nkat·g−1)过氧化氢酶/
(×16.67 nkat·g−1)ck 3 018.58±80.60 bc 43 031.11±2 231.96 c 46.81±1.47 c E3 3 219.58±106.30 a 49 306.67±1 573.26 ab 50.67±0.73 ab E1 2 978.00±102.19 c 46 871.11±1 625.14 b 46.96±2.57 c E4 3 202.99±99.55 a 48 937.78±2 994.76 ab 53.63±2.00 a E2 3 159.32±111.30 ab 46 404.44±1 779.30 b 48.44±0.73 bc E5 3 187.05±118.30 a 51 733.33±2 538.05 a 46.37±0.91 c 说明:同列比较,不同字母表示处理间差异显著(P<0.05),相同字母表示处理间差异不显著(P>0.05) -
[1] 秦忠林. 桂林毛竹林开花结实特征与实生苗需求分析[J]. 世界竹藤通讯, 2015, 13(2): 29 − 31. QIN Zhonglin. Flowering and seeding characteristics of Phyllostachys pubescens and need analysis of seedlings in Guilin [J]. World Bamboo Rattan, 2015, 13(2): 29 − 31. [2] MITCHELL J W, MANDAVA N B, WORLEY J F. Brassins: a new family of plant hormones from rape pollen [J]. Nature, 1970, 225(5237): 1065 − 1066. [3] YUAN Lingyun, SHU Shengrong, SUN Jin, et al. Effects of 24-epibrassinolide on the photosynthetic characteristics, antioxidant system, and chloroplast ultrastructure in Cucumis sativus L. under Ca(NO3)2 stress [J]. Photosynth Res, 2012, 112(3): 205 − 214. [4] YANG A J, ANJUM S A, WANG L, et al. Effect of foliar application of brassinolide on photosynthesis and chlorophyll fluorescence traits of Leymus chinensis under varying levels of shade [J]. Photosynthetica, 2018, 56: 873 − 883. [5] 张玉萍, 刘威生, 孙绍春, 等. 油菜素内酯在果树上的应用研究进展[J]. 安徽农业科学, 2010, 38(12): 91 − 92, 95. ZHANG Yuping, LIU Weisheng, SUN Shaochun, et al. Research progress on the application of brassinolide in pomology [J]. J Anhui Agric Sci, 2010, 38(12): 91 − 92, 95. [6] 邵继锋, 桂仁意, 季海宝, 等. 毛竹实生苗水培体系初步建立[J]. 浙江农林大学学报, 2011, 28(1): 86 − 94. SHAO Jifeng, GUI Renyi, JI Haibao, et al. A preliminary study on establishment of hydroponic culture system Phyllostachys edulis for seedlings [J]. J Zhejiang A&F Univ, 2011, 28(1): 86 − 94. [7] LICHTENTHALER H K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes [J]. Method Enzymol, 1987, 148(1): 350 − 382. [8] 邹琦. 植物生理生化实验指导[M]. 北京: 中国农业出版社, 1995. [9] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000. [10] 崔喜艳. 基础生物化学实验方法和技术[M]. 北京: 中国林业出版社, 2008. [11] 姜东, 于振文, 李永庚, 等. 高产小麦营养器官临时贮存物质积运及其对粒重的贡献[J]. 作物学报, 2003, 29(1): 31 − 36. JIANG Dong, YU Zhenwen, LI Yonggeng, et al. Accumulation and redistribut ion of temporal reserves in vegetative organs and its contribution to grain weight in high yield winter wheat [J]. Acta Agron Sin, 2003, 29(1): 31 − 36. [12] 王俊宁, 郑棉源, 刘付东标. 油菜素内酯对富贵竹生长的影响[J]. 亚热带植物科学, 2017, 46(4): 318 − 322. WANG Junning, ZHENG Mianyuan, LIU Fudongbiao. Effects of brassinolides on growth of Dracaena sanderiana ‘Virens’ [J]. Subtrop Plant Sci, 2017, 46(4): 318 − 322. [13] 钟妍婷, 原向阳, 刘哲, 等. 油菜素内酯处理对谷子农艺性状和生理特性的影响[J]. 作物杂志, 2015(2): 124 − 128. ZHONG Yanting, YUAN Xiangyang, LIU Zhe, et al. Effects of brassinolide on agronomic and physiological properties of foxtail millet [J]. Crops, 2015(2): 124 − 128. [14] 孔祥生, 张妙霞. 油菜素内酯和多效唑对玉米种子萌发及幼苗生长的影响[J]. 中国农学通报, 1998, 14(2): 21 − 23. KONG Xiangsheng, ZHANG Miaoxia. Effect of brassinolide and multi-ef fect-triazol on seed germination and seedling growth in corn [J]. Chin Agric Sci Bull, 1998, 14(2): 21 − 23. [15] 张慎好, 武春成, 赵振忠, 等. 油菜素内酯在黄瓜上的应用效果研究[J]. 种子, 2009, 28(6): 81 − 85. ZHANG Shenhao, WU Chuncheng, ZHAO Zhenzhong, et al. Research on application effect of brassinolide on cucumber [J]. Seed, 2009, 28(6): 81 − 85. [16] 乔晶, 胡峻, 李妍芃, 等. 油菜素内酯对甘草性状及7种化学成分含量的影响[J]. 中国中药杂志, 2016, 41(2): 197 − 204. QIAO Jing, HU Jun, LI Yanpeng, et al. Effect of exogenous brassinolide on morphological characters and contents of seven chemical constituents of Glycyrrhiza uralensis [J]. China J Chin Mater Med, 2016, 41(2): 197 − 204. [17] 张爱华. 油菜素内酯在小麦初生根发育中的作用分析[D]. 杨凌: 西北农林科技大学, 2018. ZHANG Aihua. Effects of Brassinosteroids on The Development of Wheat Primary Roots[D]. Yangling: Northwest A&F University, 2018. [18] 邓茜, 张爱华, 侯立江, 等. 油菜素内酯对小麦幼苗根系发育的影响[J]. 麦类作物学报, 2018, 38(12): 1504 − 1511. DENG Qian, ZHANG Aihua, HOU Lijiang, et al. Effects of brassinolide on development of root system of wheat seedlings [J]. J Triticeae Crop, 2018, 38(12): 1504 − 1511. [19] WANG Renhou, ESTELLE M. Diversity and specificity: auxin perception and signaling through the TIR1/AFB pathway [J]. Curr Opin Plant Biol, 2014, 21(1): 51 − 58. [20] 张海丽, 高静, 张昊, 等. 油菜素内酯对水稻细胞伸长和分裂的调控[J]. 农业生物技术学报, 2015, 23(1): 71 − 79. ZHANG Haili, GAO Jing, ZHANG Hao, et al. The regulation of brassinosteroid (BR) on elongation and division of rice (Oryza sativa) cells [J]. J Agric Biotechnol, 2015, 23(1): 71 − 79. [21] GAUDINOVÁ A, SÜSSENBEKOVÁ H, VOJTĚCHOVÁ M, et al. Different effects of two brassinosteroids on growth, auxin and cytokinin content in tobacco callus tissue [J]. Plant Growth Regul, 1995, 17(2): 121 − 126. [22] LU Zhou, HUANG Min, GE Dongping, et al. Effect of brassinolide on callus growth and regeneration in Spartina patens (Poaceae) [J]. Plant Cell Tissue Organ Culture, 2003, 73(1): 87 − 89. [23] 郑洁, 王磊. 油菜素内酯在植物生长发育中的作用机制研究进展[J]. 中国农业科技导报, 2014, 16(1): 52 − 58. ZHENG Jie, WANG Lei. Advance in mechanism of brassinosteroids in plant development [J]. J Agric Sci Technol, 2014, 16(1): 52 − 58. [24] BAO Fang, SHEN Junjiang, BRADY S R, et al. Brassinosteroids interact with auxin to pomote lateral root development in Arabidopsis [J]. Plant Physiol, 2004, 134(4): 1624 − 1631. [25] EMES M J, BOWSHER C G, HEDLEY C, et al. Starch synthesis and carbon partitioning in developing endosperm [J]. J Exper Bot, 2003, 54(382): 569 − 575. [26] 石新新, 李佐同, 杨克军, 等. 表油菜素内酯对高粱幼苗生长和光合特性的影响[J]. 黑龙江八一农垦大学学报, 2015, 27(5): 56 − 60. SHI Xinxin, LI Zuotong, YANG Kejun, et al. Effects of EBR on growth and photosynthetic system on sorghum seedlings [J]. J Heilongjiang Bayi Agric Univ, 2015, 27(5): 56 − 60. [27] 李玲, 李俊, 张春雷, 等. 外源ABA和BR在提高油菜幼苗耐渍性中的作用[J]. 中国油料作物学报, 2012, 34(5): 489 − 495. LI Ling, LI Jun, ZHANG Chunlei, et al. Effects of exogenous ABA and BR on waterlogging resistance of juvenile rapeseed [J]. Chin J Oil Crop Sci, 2012, 34(5): 489 − 495. [28] YU Jingquan, HUANG Lifeng, HU Wenhai, et al. A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus [J]. J Exper Bot, 2004, 55(399): 1135 − 1143. [29] 王金平, 张金池, 岳健敏, 等. 油菜素内酯对氯化钠胁迫下樟树幼苗光合色素和叶绿素荧光参数的影响[J]. 浙江农林大学学报, 2017, 34(1): 20 − 27. WANG Jinping, ZHANG Jinchi, YUE Jianmin, et al. BRs, photosynthetic pigments, and chlorophyll fluorescence parameters in Cinnamomum camphora seedlings with NaCl stress [J]. J Zhejiang A&F Univ, 2017, 34(1): 20 − 27. [30] 郝建军, 玄美淑, 何若韫. 油菜素内酯对玉米幼苗光合速率与呼吸速率的影响[J]. 沈阳农业大学学报, 1990, 21(1): 43 − 47. HAO Jianjun, XUAN Meishu, HE Ruoyun. Effect of brassinolide (BR) on the rate of photosynthesis and respiration in maize seed-lings [J]. J Shenyang Agric Univ, 1990, 21(1): 43 − 47. [31] BRAUN P, WILD A. The influence of brassinosteroid on growth and parameters of photosynthesis of wheat and mustard plants [J]. J Plant Physiol, 1984, 116(3): 189 − 196. [32] HASAN S A, HAYAT S, AHMAD A. Bassinosteroids protect photosynthetic machinery against the cadmium induced oxidative stress in two tomato cultivars [J]. Chemosphere, 2011, 84(10): 1446 − 1451. [33] 孙伟, 范开业, 王斌, 等. 新型植物激素油菜素内酯研究进展及在农业生产中的应用[J]. 农业科技通讯, 2013(2): 116 − 118. SUN Wei, FAN Kaiye, WANG Bin, et al. Research progress and application of new plant hormone brassinolide in agricultural production [J]. Bull Agric Sci Technol, 2013(2): 116 − 118. [34] 李蒙, 束胜, 郭世荣, 等. 24-表油菜素内酯对樱桃番茄光合特性和果实品质的影响[J]. 西北植物学报, 2015, 35(1): 138 − 145. LI Meng, SHU Sheng, GUO Shirong, et al. Effect of 24-brassinolides on photohynthetic characteristics and fruit quality of cherry tomato [J]. Acta Bot Boreali-Occident Sin, 2015, 35(1): 138 − 145. [35] 肖瑞雪, 吕静霞, 贾长松, 等. 外源油菜素内酯对油用牡丹‘凤丹’生理特性的影响[J]. 植物生理学报, 2018, 54(9): 49 − 57. XIAO Ruixue, LÜ Jingxia, JIA Changsong, et al. Effect of exogenous brassinosteroid on physiological characteristics of Paeonia ostii ‘Fengdan’ [J]. Plant Physiol J, 2018, 54(9): 49 − 57. [36] 贾东坡, 冯林剑. 植物与植物生理[M]. 重庆: 重庆大学出版社, 2015. [37] 肖瑞雪, 郭丽丽, 贾琦石, 等. 油菜素内酯调控植物生长发育及产量品质研究进展[J]. 江苏农业科学, 2019, 47(10): 16 − 21. XIAO Ruixue, GUO Lili, JIA Qishi, et al. Advances in research on brassinolide regulation of plant growth and yield and quality [J]. Jiangsu Agric Sci, 2019, 47(10): 16 − 21. [38] BAJGUZ A. Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chlorella vulgaris [J]. Plant Physiol Biochem, 2000, 38(3): 209 − 215. [39] 宋吉轩, 李金还, 刘美茹, 等. 油菜素内酯对干旱胁迫下羊草渗透调节及抗氧化酶的影响研究[J]. 草业学报, 2015, 24(8): 93 − 102. SONG Jixuan, LI Jinhuan, LIU Meiru, et al. Effects of brassinosteroid application on osmotic adjustment and antioxidant enzymes in Leymus chinensis under drought stress [J]. Acta Pratacult Sin, 2015, 24(8): 93 − 102. [40] CHAO Yunying, ZHAO Hua. Protective roles of brassinolide on rice seedlings under high temperature stress [J]. Rice Sci, 2008, 15(1): 63 − 68. [41] ALI B, HAYAT S, HASAN S, HASAN S A, et al. Effect of root applied 28-homobrassinolide on the performance of Lycopersicon esculentum [J]. Sci Hortic, 2006, 110(3): 267 − 273. [42] 王传凯, 郭淼. 油菜素内酯对裸燕麦常温和高温胁迫下生长形态及生理特性的影响[J]. 南方农业学报, 2017, 48(7): 1173 − 1177. WANG Chuankai, GUO Miao. Effect of brassinolide on growth morphology and physiological characters of Avena nuda under room and high temperature stresses [J]. J Southern Agric, 2017, 48(7): 1173 − 1177. -
链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.20200161