Difference analysis using an FTIR spectrum for individual walnuts
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摘要: 为了探索一种高效快速安全的测量核桃Juglans regia内含化学物质的方法,采用红外光谱法研究了分别采自贵州省赫章县和普定县的11个核桃单株种子化学成分的红外光谱,根据与蛋白质、脂肪、碳水化合物实测含量值相关度较高的吸光值比D(1658)/ D(3010),D(1747)/ D(3010)和D(1100)/ D(3010),对核桃种子蛋白质、脂肪和碳水化合物的相对含量进行了比较分析,并利用其红外光谱吸收峰值对11个核桃单株进行聚类分析。结果表明:以上特征峰值与用经典化学分析法测得的核桃化学成分含量值的相关系数均高于显著性水平为0.01的临界值,即可认为它们是极显著相关的。因此,傅立叶红外光谱法适宜作为一种快速高效直接的测定核桃种子物质成分组成和相对含量的方法。研究结果可在一定程度上为核桃的良种选育和亲缘关系确定提供理论依据。图2表4参16Abstract: To determine an efficient, rapid, and safe measurement for walnuts containing chemical substances, 11 individual Juglans regia walnuts from Hezhang and Puding in Guizhou Province were tested using Fourier transform infrared spectroscopy (FTIRS). A correlation analysis between FTIR absorbance ratios from the FTIR spectra [D(1658)/D(3010), D(1747)/D(3010), and D(1100)/D(3010)] and those from a conventional quantitative chemical analysis was employed. The relative ratios of the peaks in the spectra of the 11 individual walnuts were tested using ANOVA(t=11, n=5)and SNK to show differences in contents for various seed components. Results of the ratios among the main components in the walnuts obtained from the FTIR spectra were in favorable agreement with those from a conventional quantitative chemical analysis and had significant correlation coefficients (r>r0.01(9)=0.735). The relative ratios of the peaks in the spectra of the 11 individual walnuts showed various components in the seeds all had significant differences using ANOVA(P=0.01). So, as an easy, fast, and direct technique, FTIR spectroscopy could be used to characterize components and their relative content of plant seeds and could provide a theoretical foundation for breeding and revealing genetic relationships of walnuts in Guizhou. [Ch, 2 fig. 4 tab. 16 ref.]
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Key words:
- cash forestry /
- Juglans regia /
- FTIRS /
- ANOVA /
- cluster analysis
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2015.03.014
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