Detection and identification of mulberry bacterial wilt and its pathogen using Fourier transform infrared spectra
-
摘要: 为快速有效鉴别桑细菌性萎蔫病,比较了桑细菌性萎蔫病菌Enterobacter mori和引起相似田间发病症状的桑青枯病菌Ralstonia solanacearum的红外光谱。结果显示:在4 000.00~500.00 cm-1范围内,2种重要桑细菌病原存在5个共有峰,其中3个强度有显著性差异。此外,桑萎蔫病菌的红外光谱有1 399.10和1 079.45 cm-1 2个特征峰,桑青枯病菌的红外光谱有2 973.49,1 724.42,1 380.60,1 278.67,1 185.12,1 132.03,1 100.51和979.31 cm-1等8个特征峰。进一步分析和比较感染桑萎蔫病的桑枝和健康桑枝的红外光谱发现,感病枝和健康枝有5个相同的峰,其中4个强度有显著差异;此外,感病枝有1到643.92和1 407.31 cm-1 2个特征峰,健康枝中有1 635.83,1 506.36,1 423.59,1 374.97,1 328.57和1 108.82 cm-1 6个特征峰。为利用傅里叶红外光谱技术简便、直观和快速地鉴别桑细菌性萎蔫病及其病原提供了新的思路。图2表3参19Abstract: To rapidly identify a newly emerging mulberry wilt disease in Zhejiang Province, this study compared Fourier transform infrared (FTIR) spectra of Enterobacter mori and Ralstonia solanacearum, which cause similar disease symptoms on mulberry. First, FTIR spectra were taken on E. mori and R. solanacearum, and then a comparison of mulberry branches infected with and without E. mori was performed based on FTIR spectra. Results for E. mori and R. solanacearum in the 4 000.00 - 500.00 cm-1 region showed 5 similar peaks with 3 of the similar peaks differing in intensity. Peaks at 1 399.10 and 1 079.45 were specific to E. mori; whereas, peaks at 2 973.49, 1 724.42, 1 380.60, 1 278.67, 1 185.12, 1 132.03, 1 100.51, and 979.31 cm-1 were specific to R. solanacearum. The FTIR spectra for healthy and infected mulberry branches showed 5 similar peaks in the 4 000.00 - 500.00 cm-1 region with 4 of the similar peaks differing in intensity. Peaks at 1 643.92 and 1 407.31 cm-1 were specific to infected branches; whereas, peaks at 1 635.83, 1 506.36, 1 423.59, 1 374.97, 1 328.57, and 1 108.82 cm-1 were specific to healthy branches. This study indicated that Fourier transform infrared spectra could provide a simple, intuitive, and fast technology for rapid detection and identification of mulberry bacterial wilt. [Ch, 2 fig. 3 tab. 19 ref.]
-
Key words:
- plant protection /
- mulberry /
- Enterobacter mori /
- Ralstonia solanacearum /
- specific weak /
- FTIR
-
-
链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2015.04.013

计量
- 文章访问数: 1904
- HTML全文浏览量: 245
- PDF下载量: 686
- 被引次数: 0