Genetic differentiation of four straight-culmed provenances of <em>Dendrocalamus sinicus</em> based on ISSRs

YANG Han-qi; RUAN Zhen-yuan; TIAN Bo; YANG Yu-ming; SUN Mao-sheng

doi:10.11833/j.issn.2095-0756.2010.01.013

Volume 27 Issue 1

Feb. 2010

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Article Navigation > Journal of Zhejiang A&F University > 2010 > 27(1): 81-86

YANG Han-qi, RUAN Zhen-yuan, TIAN Bo, YANG Yu-ming, SUN Mao-sheng. Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs[J]. Journal of Zhejiang A&F University, 2010, 27(1): 81-86. doi: 10.11833/j.issn.2095-0756.2010.01.013

Citation:

YANG Han-qi, RUAN Zhen-yuan, TIAN Bo, YANG Yu-ming, SUN Mao-sheng. Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs[J]. Journal of Zhejiang A&F University, 2010, 27(1): 81-86. doi: 10.11833/j.issn.2095-0756.2010.01.013

Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs

doi: 10.11833/j.issn.2095-0756.2010.01.013

1.
Research Institute of Resource Insects，The Chinese Academy of Forestry，Kunming 650224，Yunnan，China;
2.
School of Resources，Southwest Forestry College，Kunming 650224，Yunnan，China;
3.
Xishuangbanna Tropical Botanical Garden，Chinese Academy of Sciences，Kunming 650223，Yunnan，China

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Corresponding author: TIAN Bo
Publish Date: 2010-02-20

Abstract

The straight-culmed type of Dendrocalamus sinicus is one of the most important economic bamboo resources in Yunnan Province，China. To protect and develop native germplasm resources of the straight-culmed D. sinicus in Yunnan，the genetic diversity of four straight-culmed provenances of D. sinicus was analyzed based on inter-simple sequence repeat（ISSR） markers for percentage of polymorphic bands（PPB），Neis gene diversity（H），Shannons information index（I） and Neis coefficient of genetic differentiation（Gst）. Results showed seven informative and reliable primers with 54 polymorphic loci that were chosen from 80 pre-screened primers. At the population level，PPB was 9.59%，H was 0.036 3，and I was 0.053 6；whereas at the species level，PPB was 73.97%，H was 0.260 0 and I was 0.3921. Also，Gst was 0.863 4. Gst indicated a high degree of genetic differentiation among straight-culmed D. sinicus provenances most likely due to habitat fragmentation and flowering without seed.［Ch，4 fig. 4 tab. 21 ref.］
- forest tree breeding,
- Dendrocalamus sinicus,
- genetic differentiation,
- inter-simple sequence repeat（ISSR）

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs

1. Research Institute of Resource Insects，The Chinese Academy of Forestry，Kunming 650224，Yunnan，China;

2. School of Resources，Southwest Forestry College，Kunming 650224，Yunnan，China;

3. Xishuangbanna Tropical Botanical Garden，Chinese Academy of Sciences，Kunming 650223，Yunnan，China

Corresponding author: TIAN Bo

Publish Date: 2010-02-20

Key words:

Abstract: The straight-culmed type of Dendrocalamus sinicus is one of the most important economic bamboo resources in Yunnan Province，China. To protect and develop native germplasm resources of the straight-culmed D. sinicus in Yunnan，the genetic diversity of four straight-culmed provenances of D. sinicus was analyzed based on inter-simple sequence repeat（ISSR） markers for percentage of polymorphic bands（PPB），Neis gene diversity（H），Shannons information index（I） and Neis coefficient of genetic differentiation（Gst）. Results showed seven informative and reliable primers with 54 polymorphic loci that were chosen from 80 pre-screened primers. At the population level，PPB was 9.59%，H was 0.036 3，and I was 0.053 6；whereas at the species level，PPB was 73.97%，H was 0.260 0 and I was 0.3921. Also，Gst was 0.863 4. Gst indicated a high degree of genetic differentiation among straight-culmed D. sinicus provenances most likely due to habitat fragmentation and flowering without seed.［Ch，4 fig. 4 tab. 21 ref.］

Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs

doi: 10.11833/j.issn.2095-0756.2010.01.013

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Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs

doi: 10.11833/j.issn.2095-0756.2010.01.013

Corresponding author: TIAN Bo

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Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs

doi: 10.11833/j.issn.2095-0756.2010.01.013

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通讯作者: 陈斌, bchen63@163.com

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Genetic differentiation of four straight-culmed provenances of Dendrocalamus sinicus based on ISSRs

doi: 10.11833/j.issn.2095-0756.2010.01.013

Corresponding author: TIAN Bo

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