Volume 27 Issue 3
Jun.  2010
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SHEN Jun-ling, NI Hui-qun, CHEN Xiao-yang, HUANG Shao-wei. Genetic diversity of Jatropha curcas with SRAP molecular markers[J]. Journal of Zhejiang A&F University, 2010, 27(3): 347-353. doi: 10.11833/j.issn.2095-0756.2010.03.005
Citation: SHEN Jun-ling, NI Hui-qun, CHEN Xiao-yang, HUANG Shao-wei. Genetic diversity of Jatropha curcas with SRAP molecular markers[J]. Journal of Zhejiang A&F University, 2010, 27(3): 347-353. doi: 10.11833/j.issn.2095-0756.2010.03.005

Genetic diversity of Jatropha curcas with SRAP molecular markers

doi: 10.11833/j.issn.2095-0756.2010.03.005
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  • Corresponding author: CHEN Xiao-yang
  • Publish Date: 2010-06-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Genetic diversity of Jatropha curcas with SRAP molecular markers

doi: 10.11833/j.issn.2095-0756.2010.03.005
    Corresponding author: CHEN Xiao-yang

Abstract: To investigate genetic relationships and genetic diversity of Jatropha curcas,seven populations from six provinces of China and one from Indonesia were analyzed using polymerase chain reaction (PCR) with sequence related amplified polymorphic (SRAP) DNA molecular markers. The L16(45) orthogonal experimental design was utilized to optimize the SRAP amplification system with Jaccards similarity coefficient as well as cluster and principle component analyses being employed. The optimum reaction concentrations in a 20 L reaction mixture were:2 L 10 PCR buffer,20 ng DNA template,16.67 nkat Taq enzyme, 2.50 mmolL-1 Mg2+,120.00 molL-1 deoxyribonucleotide triphosphate(dNTP) and 0.15 molL-1 primers. For the eight populations,using the 45 most informative primer pairs,141(28.20%) of which were polymorphic,SRAP screening produced 500 bands. Jaccards similarity coefficient showed a high similarity (0.791 - 0.940). Also,the cluster and principle component analyses showed three groupings:the Indonesian population,and seven Chinese populations were divided into two groups.[Ch,6 fig. 3 tab. 16 ref.]

SHEN Jun-ling, NI Hui-qun, CHEN Xiao-yang, HUANG Shao-wei. Genetic diversity of Jatropha curcas with SRAP molecular markers[J]. Journal of Zhejiang A&F University, 2010, 27(3): 347-353. doi: 10.11833/j.issn.2095-0756.2010.03.005
Citation: SHEN Jun-ling, NI Hui-qun, CHEN Xiao-yang, HUANG Shao-wei. Genetic diversity of Jatropha curcas with SRAP molecular markers[J]. Journal of Zhejiang A&F University, 2010, 27(3): 347-353. doi: 10.11833/j.issn.2095-0756.2010.03.005

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