Regeneration of <em>Taxus chinensis</em> var. <em>mairei </em>from adventitious bud formation using an in vitro embryo culture

ZENG Yu-li; LIN Xin-chun; GUI Ren-yi; ZHANG Cui-ping; HUANG Li-chun

doi:10.11833/j.issn.2095-0756.2010.04.023

Volume 27 Issue 4

Aug. 2010

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2010 > 27(4): 614-619

ZENG Yu-li, LIN Xin-chun, GUI Ren-yi, ZHANG Cui-ping, HUANG Li-chun. Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture[J]. Journal of Zhejiang A&F University, 2010, 27(4): 614-619. doi: 10.11833/j.issn.2095-0756.2010.04.023

Citation:

ZENG Yu-li, LIN Xin-chun, GUI Ren-yi, ZHANG Cui-ping, HUANG Li-chun. Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture[J]. Journal of Zhejiang A&F University, 2010, 27(4): 614-619. doi: 10.11833/j.issn.2095-0756.2010.04.023

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

doi: 10.11833/j.issn.2095-0756.2010.04.023

1.
The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: HUANG Li-chun
Publish Date: 2010-08-20

Abstract

The effect of seed sterilization conditions，various basal media，and growth regulators on explants of mature Taxus chinensis var. mairei embryos were studied. Results showed optimal germination occurred with seeds soaked in a 25.0 gL-1 sodium hypochlorite solution for 10 min in a vacuum. The contamination rate was reduced to 6% and the germination rate reached 86%. A woody plant medium （WPM） was selected as the best basal medium with 0.1 mgL-1 kinetin （KT） and 1.0 mgL-1 zeatin （ZT）. This medium effectively induced more clusters of adventitious bud formation；whereas 6-benzylaminopurine（6-BA） and isopenlenyladenine（2iP） induced fewer adventitious buds while N-phenyl-N-1，2，3-thidiazol-5-ylurea（TDZ） produced no adventitious bud differentiation. With WPM，germinated embryos effectively induced adventitious buds.［Ch，3 fig. 7 tab. 11 ref.］
- silviculture,
- Taxus chinensis var. mairei,
- embryo culture,
- plant growth regulators,
- adventitious buds

Relative Article

[1]	ZHOU Wenling, WEI Hongling, LI Dewen, TANG Zhonghua, LIU Ying, XIE Shengnan, TIAN Xuchen, CHU Qiming. Effects of plant growth regulators on the main secondary metabolites in Eucommia ulmoides leaves . Journal of Zhejiang A&F University, 2023, 40(5): 999-1007. doi: 10.11833/j.issn.2095-0756.20220705
[2]	ZHU Xiaoyu, TONG Wanwan, ZHAO Chu, TIAN Ru’nan. Root formation and anatomical structure of Ilex × altaclerensis ‘Belgica Aurea’ stem cuttings . Journal of Zhejiang A&F University, 2022, 39(2): 347-355. doi: 10.11833/j.issn.2095-0756.20210283
[3]	YE Wen, YUAN Chaoqun, QIN Wei, WANG Zhentao, ZHU Yuqiu. Optimization of regeneration system of stem segments of Nandina domestica 'Firepower' in vitro culture . Journal of Zhejiang A&F University, 2020, 37(2): 391-396. doi: 10.11833/j.issn.2095-0756.2020.02.026
[4]	CHEN Han, ZHANG Chao, YU Shuquan. Potential distribution area of Taxus chinensis var. mairei in Liandu District based on a Stacking algorithm . Journal of Zhejiang A&F University, 2019, 36(3): 494-500. doi: 10.11833/j.issn.2095-0756.2019.03.009
[5]	HU Tao, CAO Yu, ZHANG Gexiang. Rooting of Chionanthus virginicus hardwood cuttings with media and plant growth regulators . Journal of Zhejiang A&F University, 2019, 36(3): 622-628. doi: 10.11833/j.issn.2095-0756.2019.03.025
[6]	LI Jun, WANG Xiuhua, CHU Xiuli, ZHANG Dongbei, ZHOU Zhichun. Substrate proportion for growth and N/P absorption in two-year-old container seedlings of three precious tree species . Journal of Zhejiang A&F University, 2017, 34(6): 1044-1050. doi: 10.11833/j.issn.2095-0756.2017.06.011
[7]	OU Jiande, WU Zhizhuang. Growth and stem form quality with pruning in Taxus wallichiana var. mairei . Journal of Zhejiang A&F University, 2017, 34(1): 104-111. doi: 10.11833/j.issn.2095-0756.2017.01.015
[8]	OU Jiande, WU Zhizhuang. Excellent families and plus trees of Taxus wallichiana var. mairei for landscaping . Journal of Zhejiang A&F University, 2016, 33(1): 102-108. doi: 10.11833/j.issn.2095-0756.2016.01.014
[9]	YIN Fangfang, LIN Xiazhen, NING Mengya, HU Lipeng. Chemical reagents with Cyclobalanopsis glauca container seedlings . Journal of Zhejiang A&F University, 2015, 32(2): 244-250. doi: 10.11833/j.issn.2095-0756.2015.02.011
[10]	JING Ning, KANG Jin, ZHANG Yaohong, KANG Yongxiang. Callus induction and plant regeneration in Sinopodophyllum hexandrum . Journal of Zhejiang A&F University, 2015, 32(1): 162-166. doi: 10.11833/j.issn.2095-0756.2015.01.024
[11]	ZHANG Rui, WANG Xiu-hua, CHEN Liu-ying, FENG Jian-guo, ZHOU Zhi-chun. Growth and heartwood characteristics of Ormosia hosiei plantations . Journal of Zhejiang A&F University, 2012, 29(3): 412-419. doi: 10.11833/j.issn.2095-0756.2012.03.014
[12]	WANG Hui, LOU Lu-huan, ZHU Xiao-lou. Effects of exogenous hormones on rooting of Euonymus hamiltonianus and E. alatus cuttings . Journal of Zhejiang A&F University, 2010, 27(1): 155-158. doi: 10.11833/j.issn.2095-0756.2010.01.025
[13]	WAN Jun-li, HUANG Jian-qin, XIA Guo-hua, ZHANG Qi-xiang, HUANG Li-chun. Adventitious bud induction with immature embryo of Carya cathayensis . Journal of Zhejiang A&F University, 2009, 26(5): 762-766.
[14]	WU Jia-sen, ZHANG Li-qin, WU Jin-cai, WU Chang-yi, CHEN Rong. Macronutrients and allocations to different organs in one-to three-year-old Taxus chinensis var. mairei . Journal of Zhejiang A&F University, 2008, 25(2): 195-199.
[15]	YU Ci-ying, LI Xiu-peng, YUAN Yan-fei, CHEN Ye-ping, LI Wan-xing, MIAO Ling-xia. Ex-situ island cultivation and propagation techniques for Taxus chinensis var. mairei . Journal of Zhejiang A&F University, 2008, 25(1): 60-64.
[16]	WANG Chun, ZHENG Yong-ping, LUOMan, ZHOUFang-yong, YUQiong-fang. Micropropagation of Dendrobium officinale . Journal of Zhejiang A&F University, 2007, 24(3): 372-376.
[17]	WANG Yue-sheng, ZHOU Zhi-chun, JIN Guo-qin, HONG Gui-mu, WANG Hui. Growth of Taxus chinensis var .mairei for container seedlings in different media mixtures and for bare-root versus container seedlings in a young stand . Journal of Zhejiang A&F University, 2007, 24(5): 643-646.
[18]	CHEN Long-an, YU Xue-jun, HAN Chun, XUAN Tao-tao, Li Zhong-ju. Application of ABT growth regulator in dual purpose Phyllostachys pubescens plantation . Journal of Zhejiang A&F University, 1999, 16(2): 131-134.
[19]	Zhang Ruohui, Liu Hong'e, Cai Jianguo, Shen Xikang. Promotion of Cutting Propagation for Woody Plants by Application of New Green Plant Growth Regulators: ABT ( 11～ 15) . Journal of Zhejiang A&F University, 1998, 15(1): 22-26.
[20]	Cai Jianguo, Shen Xikang, Zhang Ruohui, Lin Dingbo, Lu Yuanyuan. Application of stereo-device for cuttage and plant growth regulators on cutting propagation of Ginkgo biloba . Journal of Zhejiang A&F University, 1998, 15(4): 340-346.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(4610) PDF downloads(315) Cited by()

Proportional views

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Li-chun

Publish Date: 2010-08-20

Key words:

Abstract: The effect of seed sterilization conditions，various basal media，and growth regulators on explants of mature Taxus chinensis var. mairei embryos were studied. Results showed optimal germination occurred with seeds soaked in a 25.0 gL-1 sodium hypochlorite solution for 10 min in a vacuum. The contamination rate was reduced to 6% and the germination rate reached 86%. A woody plant medium （WPM） was selected as the best basal medium with 0.1 mgL-1 kinetin （KT） and 1.0 mgL-1 zeatin （ZT）. This medium effectively induced more clusters of adventitious bud formation；whereas 6-benzylaminopurine（6-BA） and isopenlenyladenine（2iP） induced fewer adventitious buds while N-phenyl-N-1，2，3-thidiazol-5-ylurea（TDZ） produced no adventitious bud differentiation. With WPM，germinated embryos effectively induced adventitious buds.［Ch，3 fig. 7 tab. 11 ref.］

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

doi: 10.11833/j.issn.2095-0756.2010.04.023

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

doi: 10.11833/j.issn.2095-0756.2010.04.023

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Li-chun

HTML

Catalog

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

doi: 10.11833/j.issn.2095-0756.2010.04.023

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

doi: 10.11833/j.issn.2095-0756.2010.04.023

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Li-chun

HTML

Catalog

Export File

Citation

Format

Content