Regeneration of <em>Taxus chinensis</em> var. <em>mairei </em>from adventitious bud formation using an in vitro embryo culture

ZENG Yu-li; LIN Xin-chun; GUI Ren-yi; ZHANG Cui-ping; HUANG Li-chun

doi:10.11833/j.issn.2095-0756.2010.04.023

Volume 27 Issue 4

Aug. 2010

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ZENG Yu-li, LIN Xin-chun, GUI Ren-yi, et al. Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture[J]. Journal of Zhejiang A&F University, 2010, 27(4): 614-619. DOI: 10.11833/j.issn.2095-0756.2010.04.023

Citation:

ZENG Yu-li, LIN Xin-chun, GUI Ren-yi, et al. Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture[J]. Journal of Zhejiang A&F University, 2010, 27(4): 614-619. DOI: 10.11833/j.issn.2095-0756.2010.04.023

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

DOI: 10.11833/j.issn.2095-0756.2010.04.023

1.
The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

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Corresponding author: HUANG Li-chun
Publish Date: 2010-08-20

Abstract

The effect of seed sterilization conditions，various basal media，and growth regulators on explants of mature Taxus chinensis var. mairei embryos were studied. Results showed optimal germination occurred with seeds soaked in a 25.0 gL-1 sodium hypochlorite solution for 10 min in a vacuum. The contamination rate was reduced to 6% and the germination rate reached 86%. A woody plant medium （WPM） was selected as the best basal medium with 0.1 mgL-1 kinetin （KT） and 1.0 mgL-1 zeatin （ZT）. This medium effectively induced more clusters of adventitious bud formation；whereas 6-benzylaminopurine（6-BA） and isopenlenyladenine（2iP） induced fewer adventitious buds while N-phenyl-N-1，2，3-thidiazol-5-ylurea（TDZ） produced no adventitious bud differentiation. With WPM，germinated embryos effectively induced adventitious buds.［Ch，3 fig. 7 tab. 11 ref.］
- silviculture,
- Taxus chinensis var. mairei,
- embryo culture,
- plant growth regulators,
- adventitious buds

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Li-chun

Publish Date: 2010-08-20

Key words:

Abstract: The effect of seed sterilization conditions，various basal media，and growth regulators on explants of mature Taxus chinensis var. mairei embryos were studied. Results showed optimal germination occurred with seeds soaked in a 25.0 gL-1 sodium hypochlorite solution for 10 min in a vacuum. The contamination rate was reduced to 6% and the germination rate reached 86%. A woody plant medium （WPM） was selected as the best basal medium with 0.1 mgL-1 kinetin （KT） and 1.0 mgL-1 zeatin （ZT）. This medium effectively induced more clusters of adventitious bud formation；whereas 6-benzylaminopurine（6-BA） and isopenlenyladenine（2iP） induced fewer adventitious buds while N-phenyl-N-1，2，3-thidiazol-5-ylurea（TDZ） produced no adventitious bud differentiation. With WPM，germinated embryos effectively induced adventitious buds.［Ch，3 fig. 7 tab. 11 ref.］

Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

DOI: 10.11833/j.issn.2095-0756.2010.04.023

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

doi: 10.11833/j.issn.2095-0756.2010.04.023

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Li-chun

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Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

DOI: 10.11833/j.issn.2095-0756.2010.04.023

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通讯作者: 陈斌, bchen63@163.com

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Regeneration of Taxus chinensis var. mairei from adventitious bud formation using an in vitro embryo culture

doi: 10.11833/j.issn.2095-0756.2010.04.023

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Li-chun

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