Organic acid exudates from roots of <em>Phyllostachys pubescens</em> with aluminum stress

LIU Ying-kun; CAI Sha-yi; YU Wei-wu; LENG Hua-nan; GUI Ren-yi

doi:10.11833/j.issn.2095-0756.2011.04.002

Volume 28 Issue 4

Aug. 2011

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Article Navigation > Journal of Zhejiang A&F University > 2011 > 28(4): 533-537

LIU Ying-kun, CAI Sha-yi, YU Wei-wu, LENG Hua-nan, GUI Ren-yi. Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress[J]. Journal of Zhejiang A&F University, 2011, 28(4): 533-537. doi: 10.11833/j.issn.2095-0756.2011.04.002

Citation:

LIU Ying-kun, CAI Sha-yi, YU Wei-wu, LENG Hua-nan, GUI Ren-yi. Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress[J]. Journal of Zhejiang A&F University, 2011, 28(4): 533-537. doi: 10.11833/j.issn.2095-0756.2011.04.002

Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

doi: 10.11833/j.issn.2095-0756.2011.04.002

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University， Lin’an 311300，Zhejiang，China;
2.
Forest Enterprise of Huzhou City，Huzhou 313000，Zhejiang，China

More Information

Corresponding author: GUI Ren-yi
Publish Date: 2011-08-20

Abstract

To develop a simple method to determine the organic acids exuded from roots of Phyllostachys pubescens，root exudates were obtained using a water culture method with treatments of 0，100，500，1 000，and 2 000 molL－1 AlCl3 as the culture solution and analyzed through ultra performance liquid chromatography（UPLC） with ultraviolet（UV） detection. The exudate was concentrated in a rotary evaporator，and separation was performed on an ACQUITY UPLC high-strength silica（HSS） T3 C18 column with 3% CH3OH-KH2PO4（0.01 molL-1）（pH 2.6）（V/V） as the mobile phase of an ACQUITY TM UPLC system. Analysis included the effect on separation of 4 organic acids（oxalic acid，malic acid，citric acid and succinic acid）using UV detection wavelength，chromatographic column，and the pH of the mobile phase. Results showed favorable recovery（95％－105%），satisfactory precision （RSD ＜ 2%），and a good linear relationship （0.022－1.100 g for the 4 organic acids）. According to the retention time and the spectrum of the analytes，the organic acids exuded were an oxalic acid and malic acid. The Al3+ ions interacted with organic acid exudates with the strongest interaction when the concentration of AlCl3 was 500 molL-1. ［Ch，2 fig. 2 tab. 14 ref.］
- botany,
- ultra performance liquid chromatography（UPLC）,
- aluminum stress,
- Phyllostachys pubescens,
- organic acid

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University， Lin’an 311300，Zhejiang，China;

2. Forest Enterprise of Huzhou City，Huzhou 313000，Zhejiang，China

Corresponding author: GUI Ren-yi

Publish Date: 2011-08-20

Key words:

botany /
ultra performance liquid chromatography（UPLC） /
aluminum stress /
Phyllostachys pubescens /
organic acid

Abstract: To develop a simple method to determine the organic acids exuded from roots of Phyllostachys pubescens，root exudates were obtained using a water culture method with treatments of 0，100，500，1 000，and 2 000 molL－1 AlCl3 as the culture solution and analyzed through ultra performance liquid chromatography（UPLC） with ultraviolet（UV） detection. The exudate was concentrated in a rotary evaporator，and separation was performed on an ACQUITY UPLC high-strength silica（HSS） T3 C18 column with 3% CH3OH-KH2PO4（0.01 molL-1）（pH 2.6）（V/V） as the mobile phase of an ACQUITY TM UPLC system. Analysis included the effect on separation of 4 organic acids（oxalic acid，malic acid，citric acid and succinic acid）using UV detection wavelength，chromatographic column，and the pH of the mobile phase. Results showed favorable recovery（95％－105%），satisfactory precision （RSD ＜ 2%），and a good linear relationship （0.022－1.100 g for the 4 organic acids）. According to the retention time and the spectrum of the analytes，the organic acids exuded were an oxalic acid and malic acid. The Al3+ ions interacted with organic acid exudates with the strongest interaction when the concentration of AlCl3 was 500 molL-1. ［Ch，2 fig. 2 tab. 14 ref.］

Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

doi: 10.11833/j.issn.2095-0756.2011.04.002

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

doi: 10.11833/j.issn.2095-0756.2011.04.002

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University， Lin’an 311300，Zhejiang，China;

2. Forest Enterprise of Huzhou City，Huzhou 313000，Zhejiang，China

Corresponding author: GUI Ren-yi

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Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

doi: 10.11833/j.issn.2095-0756.2011.04.002

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

doi: 10.11833/j.issn.2095-0756.2011.04.002

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University， Lin’an 311300，Zhejiang，China; 2. Forest Enterprise of Huzhou City，Huzhou 313000，Zhejiang，China

Corresponding author: GUI Ren-yi

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1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University， Lin’an 311300，Zhejiang，China;

2. Forest Enterprise of Huzhou City，Huzhou 313000，Zhejiang，China