Volume 28 Issue 4
Aug.  2011
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LIU Ying-kun, CAI Sha-yi, YU Wei-wu, LENG Hua-nan, GUI Ren-yi. Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress[J]. Journal of Zhejiang A&F University, 2011, 28(4): 533-537. doi: 10.11833/j.issn.2095-0756.2011.04.002
Citation: LIU Ying-kun, CAI Sha-yi, YU Wei-wu, LENG Hua-nan, GUI Ren-yi. Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress[J]. Journal of Zhejiang A&F University, 2011, 28(4): 533-537. doi: 10.11833/j.issn.2095-0756.2011.04.002

Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

doi: 10.11833/j.issn.2095-0756.2011.04.002
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  • Corresponding author: GUI Ren-yi
  • Publish Date: 2011-08-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress

doi: 10.11833/j.issn.2095-0756.2011.04.002
    Corresponding author: GUI Ren-yi

Abstract: To develop a simple method to determine the organic acids exuded from roots of Phyllostachys pubescens,root exudates were obtained using a water culture method with treatments of 0,100,500,1 000,and 2 000 molL-1 AlCl3 as the culture solution and analyzed through ultra performance liquid chromatography(UPLC) with ultraviolet(UV) detection. The exudate was concentrated in a rotary evaporator,and separation was performed on an ACQUITY UPLC high-strength silica(HSS) T3 C18 column with 3% CH3OH-KH2PO4(0.01 molL-1)(pH 2.6)(V/V) as the mobile phase of an ACQUITY TM UPLC system. Analysis included the effect on separation of 4 organic acids(oxalic acid,malic acid,citric acid and succinic acid)using UV detection wavelength,chromatographic column,and the pH of the mobile phase. Results showed favorable recovery(95%-105%),satisfactory precision (RSD < 2%),and a good linear relationship (0.022-1.100 g for the 4 organic acids). According to the retention time and the spectrum of the analytes,the organic acids exuded were an oxalic acid and malic acid. The Al3+ ions interacted with organic acid exudates with the strongest interaction when the concentration of AlCl3 was 500 molL-1. [Ch,2 fig. 2 tab. 14 ref.]

LIU Ying-kun, CAI Sha-yi, YU Wei-wu, LENG Hua-nan, GUI Ren-yi. Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress[J]. Journal of Zhejiang A&F University, 2011, 28(4): 533-537. doi: 10.11833/j.issn.2095-0756.2011.04.002
Citation: LIU Ying-kun, CAI Sha-yi, YU Wei-wu, LENG Hua-nan, GUI Ren-yi. Organic acid exudates from roots of Phyllostachys pubescens with aluminum stress[J]. Journal of Zhejiang A&F University, 2011, 28(4): 533-537. doi: 10.11833/j.issn.2095-0756.2011.04.002

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