Volume 28 Issue 6
Dec.  2011
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HU Heng-kang, JIANG Xiang-mei, ZHANG Qi-xiang, CHEN Bei, HUANG Jian-qin. Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources[J]. Journal of Zhejiang A&F University, 2011, 28(6): 911-917. doi: 10.11833/j.issn.2095-0756.2011.06.012
Citation: HU Heng-kang, JIANG Xiang-mei, ZHANG Qi-xiang, CHEN Bei, HUANG Jian-qin. Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources[J]. Journal of Zhejiang A&F University, 2011, 28(6): 911-917. doi: 10.11833/j.issn.2095-0756.2011.06.012

Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources

doi: 10.11833/j.issn.2095-0756.2011.06.012
  • Publish Date: 2011-12-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources

doi: 10.11833/j.issn.2095-0756.2011.06.012

Abstract: A process for studying somatic embryogenesis and plant regeneration in vitro from immature embryos of Carya cathayensis,which were 10-12 weeks post-pollination,was developed. To select a carbon source (glucose,sucrose,trehalose,and maltose) for embryonic callus,a single-factor experimental design was used. Then,the optimal concentration of glucose (10,20,40,60 and 80 gL-1) was determined. Also,for somatic embryogenesis,the best carbon source was decided. Then,the optimal sucrose concentrations (15,30,45,60 and 75 gL-1) for somatic embryogenesis and for somatic embryo germination were determined. Results showed that the embryogenic callus induction rate of immature embryos inoculated in the medium supplemented with glucose was significantly higher (P<0.05) than sucrose,trehalose,and maltose. Embryogenic callus percentage was significantly greater (P<0.05) with 20 gL-1 glucose with a 50.0%. For somatic embryogenesis of immature embryos,sucrose as a carbon source was significantly better(P<0.05) than glucose,trehalose,and maltose. Somatic embryogenesis induction was significantly higher (P<0.05) with 45 gL-1 sucrose. For somatic embryo germination,a basal woody plant medium (WPM) supplemented with 5 gL-1 sucrose was significantly higher (P<0.05) than other concentrations. [Ch, 6 fig. 26 ref.]

HU Heng-kang, JIANG Xiang-mei, ZHANG Qi-xiang, CHEN Bei, HUANG Jian-qin. Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources[J]. Journal of Zhejiang A&F University, 2011, 28(6): 911-917. doi: 10.11833/j.issn.2095-0756.2011.06.012
Citation: HU Heng-kang, JIANG Xiang-mei, ZHANG Qi-xiang, CHEN Bei, HUANG Jian-qin. Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources[J]. Journal of Zhejiang A&F University, 2011, 28(6): 911-917. doi: 10.11833/j.issn.2095-0756.2011.06.012

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