Volume 30 Issue 6
Nov.  2013
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ZHU Longfei, XU Yingwu, LIN Xinchun. Cloning and analysis of the flowering-related gene,BoAP3,in Bambusa oldhamii[J]. Journal of Zhejiang A&F University, 2013, 30(6): 839-843. doi: 10.11833/j.issn.2095-0756.2013.06.006
Citation: ZHU Longfei, XU Yingwu, LIN Xinchun. Cloning and analysis of the flowering-related gene,BoAP3,in Bambusa oldhamii[J]. Journal of Zhejiang A&F University, 2013, 30(6): 839-843. doi: 10.11833/j.issn.2095-0756.2013.06.006

Cloning and analysis of the flowering-related gene,BoAP3,in Bambusa oldhamii

doi: 10.11833/j.issn.2095-0756.2013.06.006
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  • Corresponding author: LIN Xinchun
  • Received Date: 2012-11-21
  • Rev Recd Date: 2013-01-07
  • Publish Date: 2013-12-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Cloning and analysis of the flowering-related gene,BoAP3,in Bambusa oldhamii

doi: 10.11833/j.issn.2095-0756.2013.06.006
    Corresponding author: LIN Xinchun

Abstract: MADS-box family genes play important roles in the determination of floral meristem identity and the development of floral organ identity. Here we reported a full length MADS-box gene obtained from the flower bud of Bambusa oldhamii. It was named as BoAP3 based on sequence similarity. DNA sequence analysis showed that the BoAP3 was 654 bp length and contained an open reading flame(ORF) encoding a protein of 218 mino acids that shared over 80% homology with Oryza sativa and Triticum aestivum of APETALA3 homologues. The protein showed a typical MADS-box gene structure containing MADS domain,K domain,I region and C terminus. Quantitative real-time PCR analysis revealed that the expression level of BoAP3 in flower buds was 8.1 times higher than in vegetative shoots,suggested that BoAP3 may be involved in floral organ development. [Ch,4 fig. 14 ref.]

ZHU Longfei, XU Yingwu, LIN Xinchun. Cloning and analysis of the flowering-related gene,BoAP3,in Bambusa oldhamii[J]. Journal of Zhejiang A&F University, 2013, 30(6): 839-843. doi: 10.11833/j.issn.2095-0756.2013.06.006
Citation: ZHU Longfei, XU Yingwu, LIN Xinchun. Cloning and analysis of the flowering-related gene,BoAP3,in Bambusa oldhamii[J]. Journal of Zhejiang A&F University, 2013, 30(6): 839-843. doi: 10.11833/j.issn.2095-0756.2013.06.006

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